Influence of avian leukosis virus long terminal repeat on biological activities of Marek's disease virus

Sun, Peng; Cui, Ning; Su, Shuai; Chen, Zimeng; Li, Yanpeng; Ding, Jiabo; Cui, Zhizhong

doi:10.1080/03079457.2015.1042835

Cited by 4 publications

(1 citation statement)

References 25 publications

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“…The LTR sequence is another major factor, which affects viral pathogenicity. Previous studies have shown that LTR consisted of 3′ unique region (U3), repeat region (R) and 5′ unique region (U5), functioned as the viral promoter; and played an important role of genetic expression regulation [21,38]. Among those regions, several regulatory elements and transcription factor binding sites were distributed in U3, including Y box, CAAT enhancer box, CArG box, and PRE box, which were relevant with viral replication, translation, and tumorigenesis [39,40].…”

Section: Discussionmentioning

confidence: 99%

Role of env gene and LTR sequence in the pathogenesis of subgroup K avian leukosis virus

Liu

Lin

et al. 2022

Journal of General Virology

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Avian leukosis virus (ALV) is a retrovirus that induces tumours in infected birds; ALV is divided into different subgroups according to the env gene and cellular tropism. In general, ALV subgroup J (ALV-J) is considered to be the most pathogenic and prevalent subgroup while subgroup K (ALV-K), a newly identified subgroup, only causes mild symptoms. To illuminate the roles of the env viral gene and LTR sequence in pathogenic differences between ALV-J and ALV-K, rescued ALV-J strain rSDAU1005, rescued ALV-K strain rJS11C1, and recombinant strains rENV(J)-LTR(K) and rENV(K)-LTR(J) were characterized and investigated in this study. Among rescued viruses, rSDAU1005 had the highest replication efficiency while rJS11C1 replicated the slowest (replication efficiency rankings were rSDAU1005 >rENV(K)-LTR(J)>rENV(J)-LTR(K)>rJS11 C1). The luciferase reporter gene assay results showed that the promoter activity of ALV-K LTR was lower than that of the ALV-J LTR promoter, which may have accounted for the slower replication efficiency of ALV-K. Pathogenicity of the four rescued viruses was determined via inoculating the yolk sacs of specific-pathogen-free chickens. The results demonstrated that all four viruses were pathogenic; rSDAU1005 caused the most severe growth retardation and immunosuppression. rENV(J)-LTR(K) was more pathogenic when compared to rENV(K)-LTR(J), indicating that env and the LTR sequence play important roles in pathogenicity between ALV-K and ALV-J. Additionally, env seemed to especially play a role in ALV-K pathogenesis. This study provided scientific data and insight to improve detection methods and judgement criteria in ALV clearance and surveillance.

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Section: Discussionmentioning

confidence: 99%

Role of env gene and LTR sequence in the pathogenesis of subgroup K avian leukosis virus

Liu

Lin

et al. 2022

Journal of General Virology

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Characterization of Md5-BAC-REV-LTR virus as Marek’s disease vaccine in commercial meat-type chickens: protection and immunosuppression

et al. 2021

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The molecular basis for host responses to Marek's disease viruses integrated with different retro-viral long terminal repeat

Zhou

Cui

et al. 2018

Poultry Science

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Integration of retro-viral long terminal repeat (LTR) into the Marek's disease virus (MDV) genome can occur both in co-cultivation cell cultures and naturally in dual infected chickens. It is clear that the LTR insert is associated with the pathogenicity of MDV. The objective of this study was to compare the host responses to MDV with a different retro-viral LTR insert. Gene-chip containing chicken genome was employed to investigate the gene transcription profile of chicken embryo fibroblasts cells, and 795 genes were differentially expressed in chicken embryo fibroblasts infected with GX0101 with a reticuloendotheliosis virus LTR insert as compared to GX0101-ALV-LTR significantly. The differentially expressed genes were mostly associated with the regulation of transcription and the development of multiple organs. Based on the bio functions of the differential genes, infection of GX0101 was predicated with a greater development disorder of multiple systems, resulting in higher growth retardation, mortality, tumorigenicity, and immunosuppression in chickens than GX0101-ALV-LTR. Collectively, our results provided valuable insights into elucidation of the possible relationship between retro-viral LTR insert and the observed phenotypes caused by MDV recombinant viruses.

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Influence of avian leukosis virus long terminal repeat on biological activities of Marek's disease virus

Cited by 4 publications

References 25 publications

Role of env gene and LTR sequence in the pathogenesis of subgroup K avian leukosis virus

Role of env gene and LTR sequence in the pathogenesis of subgroup K avian leukosis virus

Characterization of Md5-BAC-REV-LTR virus as Marek’s disease vaccine in commercial meat-type chickens: protection and immunosuppression

The molecular basis for host responses to Marek's disease viruses integrated with different retro-viral long terminal repeat

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