Complete Genome Sequence of a Porcine Epidemic Diarrhea Virus Variant

Chen, Jianfei; Liu, Xiaozhen; Da, Shi; Shi, Hongyan; Zhang, Xin; Feng, Li

doi:10.1128/jvi.07150-11

Cited by 61 publications

(58 citation statements)

References 5 publications

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“…Most of the diversity between the recent field strains in G1-1 and in G2 is located within the 1100 nt-domain. The phenomenon is also found in the S gene of CH/FJND-3/2011 [17]. …”

Section: Discussionmentioning

confidence: 88%

“…Our previously study indicated that the S gene of CH/FJND-3/2011) has the lowest sequence homology (93.75%) with that of CH/S and is 9 nt longer than those of CV777 and CH/S [17]. A more precise study with the full-length S genes of recently Chinese field strains will help to best understand the genetic relationships among prevailing PEDV in China.…”

Section: Discussionmentioning

confidence: 99%

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Detection and Molecular Diversity of Spike Gene of Porcine Epidemic Diarrhea Virus in China

Chen

Liu

et al. 2013

Viruses

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Since late 2010, porcine epidemic diarrhea virus (PEDV) has rapidly disseminated all over the China and caused considerable morbidity and high mortality (up to 100%) in neonatal piglets. 79.66% (141 of 177) pig farms in 29 provinces (excluding Tibet and Hainan, China) and 72.27% (417 of 577) samples were positive for PEDV confirmed by reverse transcription-polymerase chain reaction (RT-PCR). The full-length S genes of representative field strains were sequenced. 33 field strains share 93.5%–99.9% homologies with each other at the nucleotide sequence level and 92.3%–99.8% homologies with each other at the amino acids sequence level. Most field strains have nucleotide deletion and insertion regions, and show lower homologies (93.5%–94.2%) with Chinese classical strain CH/S, however higher homologies (97.1%–99.3%) with recent strain CHGD-1. The phylogenetic analysis showed there are classical strains and variants prevailing in pig herd in China. PEDV has a high detection rate in pig herds in China. Sequence analysis indicated the S genes of recent field strains have heterogeneity and the variants are predominant.

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“…Most of the diversity between the recent field strains in G1-1 and in G2 is located within the 1100 nt-domain. The phenomenon is also found in the S gene of CH/FJND-3/2011 [17]. …”

Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Detection and Molecular Diversity of Spike Gene of Porcine Epidemic Diarrhea Virus in China

Chen

Liu

et al. 2013

Viruses

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“…However, since December 2010, large‐scale outbreaks of diarrhea with 80–100% morbidity and 50–90% mortality in suckling piglets have occurred in most Chinese provinces, affecting even vaccinated pigs . Investigations indicated that these large‐scale outbreaks of diarrhea were caused by a variant of PEDV . New PEDV outbreaks have also occurred in Vietnam, Thailand, Korea, and the United States, where PEDV was considered an exotic before 2013.…”

Section: Introductionmentioning

confidence: 99%

Proteome analysis of porcine epidemic diarrhea virus (PEDV)‐infected Vero cells

et al. 2015

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Porcine epidemic diarrhea virus (PEDV) causes an acute, highly contagious, and devastating viral enteric disease with a high mortality rate in suckling pigs. A large-scale outbreak of PED occurred in China in 2010, with PEDV emerging in the United States in 2013 and spreading rapidly, posing significant economic and public health concerns. In this study, LC-MS/MS coupled to iTRAQ labeling was used to quantitatively identify differentially expressed cellular proteins in PEDV-infected Vero cells. We identified 49 differentially expressed cellular proteins, of which 8 were upregulated and 41 downregulated. These differentially expressed proteins were involved in apoptosis, signal transduction, and stress responses. Based on these differentially expressed proteins, we propose that PEDV might utilize apoptosis and extracellular signal regulated kinases pathways for maximum viral replication. Our study is the first attempt to analyze the protein profile of PEDV-infected cells by quantitative proteomics, and we believe our findings provide valuable information with respect to better understanding the host response to PEDV infection.

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“…Porcine epidemic diarrhea virus (PEDV), a member of the alphacoronaviruses (family Coronaviridae, order Nidovirales), is a singlestranded, positive-sense RNA virus that causes disease in swine with great financial losses (Chen et al, 2012). The PEDV genome comprises two overlapping open reading frames (ORFs) encoding two polyproteins (pp1a and pp1ab) and five other proteins, spike (S), ORF3, envelope (E), membrane (M), and nucleocapsid (N).…”

Section: Introductionmentioning

confidence: 99%

Cholesterol 25-hydroxylase negatively regulates porcine intestinal coronavirus replication by the production of 25-hydroxycholesterol

Zhang

Song

Wang

et al. 2019

Veterinary Microbiology

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Cholesterol 25-hydroxylase Porcine epidemic diarrhea virus 25-hydroxycholesterol Porcine transmissible gastroenteritis virus A B S T R A C TCholesterol 25-hydroxylase (CH25H) has been shown lately to be a host restriction factor that encodes an enzyme, which catalyzes the oxidized form of cholesterol to 25-hydroxycholesterol (25HC). A series of studies have shown that 25HC activity in hosts plays a vital role in inhibiting viral infection. In this study, we explored the antiviral effect of CH25H and 25HC on porcine epidemic diarrhea virus (PEDV), which causes high mortality rates in newborn piglets with severe diarrhea, and considerable financial loss in the swine industry worldwide. Our results showed that PEDV infection downregulated the expression of CH25H in Vero cells. An overexpression and knockdown assay indicated that CH25H has significant antiviral action against PEDV, and a CH25H mutant (CH25H-M) that lacks hydroxylase activity also retains antiviral activity to a lesser extent. Furthermore, 25HC had a broad-spectrum antiviral effect against different PEDV strains by blocking viral entry. In addition, CH25H and 25HC inhibited the replication of porcine transmissible gastroenteritis virus (TGEV). Taken together, CH25H as a natural host restriction factor could inhibit PEDV and TGEV infection.

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Complete Genome Sequence of a Porcine Epidemic Diarrhea Virus Variant

Cited by 61 publications

References 5 publications

Detection and Molecular Diversity of Spike Gene of Porcine Epidemic Diarrhea Virus in China

Detection and Molecular Diversity of Spike Gene of Porcine Epidemic Diarrhea Virus in China

Proteome analysis of porcine epidemic diarrhea virus (PEDV)‐infected Vero cells

Cholesterol 25-hydroxylase negatively regulates porcine intestinal coronavirus replication by the production of 25-hydroxycholesterol

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