Complete Genome Sequence of a Peste des Petits Ruminants Virus Recovered from an Alpine Goat during an Outbreak in Morocco in 2008

Abstract

Here, we announce the first complete genome sequence of a field isolate of a peste des petits ruminants virus (PPRV) from northern Africa. This isolate is derived from an Alpine goat that suffered from severe clinical disease during the 2008 outbreak in Morocco. The full genome sequence of this isolate clusters phylogenetically with the lineage IV isolates of PPRV, sharing high levels of sequence identity with other lineage IV isolates.

Emergence of Lineage IV Peste des Petits Ruminants Virus in Ethiopia: Complete Genome Sequence of an Ethiopian Isolate 2010

“…(Kwiatek et al., ; Banyard et al., ). We have recently reported a full genome sequence of lineage IV PPRV from Morocco (Muniraju et al., ), and these two isolates are closely related with 97.9% homology. At the nucleotide level, Ethiopia/2010 shared 97% homology with Turkey/2000, 96.8–96.9% homology with Tibet/30/2007, Tibet/33/2007 and Tibet/bharal/2008, 97.6% with Sungri 1996, 92.4% with Nigeria/1976/1 and Nigeria/1975/1 and 89.3% homology with Côte d' Ivoire/1989.…”

“…PPRV specific primers were used to generate twelve overlapping amplicons covering the entire genome of the isolate. Amplicons were gel purified and sequenced using an ABI‐3730 automated sequencer (Applied‐Biosystems, Foster City, CA, USA) as described previously (Muniraju et al., ). The genome termini were determined using 3′/5′RACE (Li et al., ).…”

Molecular Evolution of Peste des Petits Ruminants Virus

“…Complete genome sequencing of 7 PPRV isolates (4 from lineage III and 3 from lineage IV) was performed according to the methods described by Muniraju et al ( 12 ). Detailed information for each of the isolates is shown in Table 1.…”

Future research to underpin successful peste des petits ruminants virus (PPRV) eradication

“…There is no evidence that these lineages vary in their pathogenicity -rather they are a reflection of the distinct geographical origins of the viruses (allopatricity) and allow limited conclusions to be drawn as to the origin of new outbreaks, e.g. it was clear that the virus that caused the first outbreaks in Africa north of the Sahara was lineage IV, and so must have come from Turkey or the Middle East, since only lineages I, II and III were circulating in sub-Saharan Africa at that time [16,17].…”