Complete genome sequence and recombination analysis of infectious bronchitis virus attenuated vaccine strain H120

Zhang, Yi; Wang, Hongning; Wang, Ting; Fan, Wen-qiao; Zhang, Anyun; Wei, Kun; Tian, Guo‐Bao; Yang, Xin

doi:10.1007/s11262-010-0517-0

Cited by 41 publications

(36 citation statements)

References 29 publications

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“…That observation was also reported by Zhang et al [23]. It is interesting that Mass/H52 (8 transferred fragments) and Mass/H120 (6 transferred fragments) share only 5 identical transferred fragments.…”

Section: Resultssupporting

confidence: 85%

Recombination in Avian Gamma-Coronavirus Infectious Bronchitis Virus

Thor

Hilt

Kissinger

et al. 2011

Viruses

100

101

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Recombination in the family Coronaviridae has been well documented and is thought to be a contributing factor in the emergence and evolution of different coronaviral genotypes as well as different species of coronavirus. However, there are limited data available on the frequency and extent of recombination in coronaviruses in nature and particularly for the avian gamma-coronaviruses where only recently the emergence of a turkey coronavirus has been attributed solely to recombination. In this study, the full-length genomes of eight avian gamma-coronavirus infectious bronchitis virus (IBV) isolates were sequenced and along with other full-length IBV genomes available from GenBank were analyzed for recombination. Evidence of recombination was found in every sequence analyzed and was distributed throughout the entire genome. Areas that have the highest occurrence of recombination are located in regions of the genome that code for nonstructural proteins 2, 3 and 16, and the structural spike glycoprotein. The extent of the recombination observed, suggests that this may be one of the principal mechanisms for generating genetic and antigenic diversity within IBV. These data indicate that reticulate evolutionary change due to recombination in IBV, likely plays a major role in the origin and adaptation of the virus leading to new genetic types and strains of the virus.

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Section: Resultssupporting

confidence: 85%

Recombination in Avian Gamma-Coronavirus Infectious Bronchitis Virus

Thor

Hilt

Kissinger

et al. 2011

Viruses

100

101

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“…The length of the genome is consistent with those previously reported for IBV [8,23,24], with a small size variation due to few insertions and deletions of nucleotide sequences in different genes and in the untranslated regions. The length of the genome is consistent with those previously reported for IBV [8,23,24], with a small size variation due to few insertions and deletions of nucleotide sequences in different genes and in the untranslated regions.…”

Section: Infectious Bronchitis Virus (Ibv) Belongs To the Ordersupporting

confidence: 89%

“…The genome organization of IBV isolate CK/SWE/0658946/10 was found to be 5 0 -Pol-S-3a-3b-E-M-5a-5b-N-UTR3 0 . Our findings show that the genome organisation of the sequenced European QX-like genotype is consistent with that of classical IBV [8,23,24]. The existence of two IBV genotypes differing in their genome organization has been reported previously, i.e., classical IBV, a group 3 gammacoronavirus (5 0 -Pol-S-3a-3b-E-M-5a-5b-N-UTR3 0 ) and a novel IBV genotype (5 0 -Pol-S-X1-E-M-N-UTR3 0 or 5 0 -Pol-S-X1-E-M-5b-N-UTR3 0 ) [26].…”

Section: Infectious Bronchitis Virus (Ibv) Belongs To the Ordersupporting

confidence: 71%

Characterization and analysis of the full-length genome of a strain of the European QX-like genotype of infectious bronchitis virus

et al. 2012

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In recent years, strains of infectious bronchitis virus belonging to the QX-like genotype have been causing huge economic losses in commercial chicken flocks in different countries in Europe. In order to expand the knowledge of the molecular features of these viruses, we have sequenced and characterized the complete genome of European QX-like IBV strain CK/SWE/0658946/10, which was isolated in 2010 in Sweden. The genome is 27664 nucleotides in length, comprising six genes and 5' and 3' untranslated regions. The ORF1a, spike and nucleocapsid genes were under strong positive selective pressure that resulted in genetic diversity in relation to classical IBV isolates. The full-length genome of the CK/SWE/0658946/10 strain has the highest nucleotide sequence identity (93.18%) to ITA/90254/2005 and the lowest nucleotide identity (89.10%) to strain CQ04-1. Phylogenetic analysis of partial S1 gene sequences of IBV strains showed that the European QX-like genotype comprises strains that have been predominantly circulating in this continent for the past decade.

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“…Recombination events associated with H120 have been recently shown by complete genome analysis of this vaccine strain and new IBV variants (Zhang et al, 2010). Our analysis of a relatively short 5?-terminal region of the S1 gene revealed crossover events involving four IBV genotypes (Mass, QX, 793/B and D274).…”

Section: á49mentioning

confidence: 52%

Molecular characterization of infectious bronchitis virus isolates from Russia and neighbouring countries: identification of intertypic recombination in the S1 gene

et al. 2011

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Infectious bronchitis virus (IBV) isolates recovered in Russia, Ukraine, and Kazakhstan between 2007 and 2010 were subjected to molecular characterization and compared with those isolated a decade ago. The IBV genome was detected in 202 out of 605 field samples from chickens with various clinical signs. Partial sequencing of the S1 gene revealed 153 vaccine strains and 49 field isolates of several genetic groups. Massachusetts, 793/B and D274 remained the predominant IBV genotypes along with QX, whereas B1648, Italy-02, Arkansas and variants accounted for about 12% of the total number. Three IBVs contained recombinant S1 gene sequences comprising genome fragments of QX-type field isolates and vaccine strains H120 (UKR/02/2009) or 4/91 (RF/03/2010), and vaccine strains H120 and D274 (RF/01/2010). The results of the present study showed a significant decline in prevalence of variant IBVs and a further spread of QXtype isolates in commercial chicken flocks in Russia as compared with the 1998 to 2002 data.

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Complete genome sequence and recombination analysis of infectious bronchitis virus attenuated vaccine strain H120

Cited by 41 publications

References 29 publications

Recombination in Avian Gamma-Coronavirus Infectious Bronchitis Virus

Recombination in Avian Gamma-Coronavirus Infectious Bronchitis Virus

Characterization and analysis of the full-length genome of a strain of the European QX-like genotype of infectious bronchitis virus

Molecular characterization of infectious bronchitis virus isolates from Russia and neighbouring countries: identification of intertypic recombination in the S1 gene

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