Molecular characterization of infectious bronchitis virus isolates from Russia and neighbouring countries: identification of intertypic recombination in the S1 gene

Овчинникова, Е. В.; Bochkov, Yury A.; Shcherbakova, L. O.; Никонова, З. Б.; Zinyakov, N. G.; Elatkin, N.P.; Мудрак, Н. С.; Борисов, А. В.; Drygin, V. V.

doi:10.1080/03079457.2011.605782

Cited by 25 publications

(25 citation statements)

References 24 publications

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“…It has been reported that the side effects of the 4/91 vaccine might provide the basis for recombination with other strains. This phenomenon has been shown by several studies which have traced the emergence of many novel IBV strains to recombination events between the 4/91 vaccine and field strains (Feng et al, 2018;Han et al, 2017;Jiang et al, 2017Jiang et al, , 2018Liu et al, 2013;Mo et al, 2013;Ovchinnikova et al, 2011;Zhang et al, 2015Zhang et al, , 2016Zhou et al, 2017) in cases where the 4/91 vaccine strain could not provide effective protection against the field strains , leading to coinfection of the vaccine and field strains in the same chickens and ultimately resulting in recombination. In this study, we performed complete genome sequence analysis of strains I0718/17, I0722/17, I0724/17, and I0737/17 and confirmed the occurrence of recombination events at the 5′ ends of the S1 gene between a 4/91-like virus (GI-13 lineage) and a YX10-like virus (GI-19) (Xu et al, 2018), which contributed to the emergence of the IBV strains.…”

Section: Discussionmentioning

confidence: 87%

Genetic and biological characteristics of four novel recombinant avian infectious bronchitis viruses isolated in China

Ren

Sheng

et al. 2019

Virus Research

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A B S T R A C TInfectious bronchitis viruses (IBVs) of GI-13 (793/B) and GI-19 (QX/LX4) lineages have been frequently detected in China in recent years. Naturally recombinant IBVs originating from the GI-13 and GI-19 lineages have also been isolated from chicken flocks with respiratory and renal problems in China. Thorough genetic and biological investigations of these recombinant viruses have led to speculation regarding their origin, evolution, and control. In order to confirm the previous results and further extend our understanding about the characteristics of the four recombinant IBV strains we had previously identified (I0718/17, I0722/17, I0724/17, and I0737/17), we conducted phylogenetic analysis by comparing their complete S1 gene sequences with those of 71 reference strains of different genotypes and lineages. We identified a close relationship between the S1 sequences of the four strains and those of GI-13 strains. The results of complete genome sequence analysis confirmed the previously identified recombination events in the four IBV strains and revealed additional recombination events in different genomic regions of strains I0718/17 and I0724/17, suggesting that the two strains originated from multiple recombination events between 4/91-like and YX10-like viruses. We comparatively evaluated the antigenicity, pathogenicity, and affinity of the four recombinant viruses and their deduced parental strains in the trachea and kidneys. Some of the strains showed comparable antigenic relatedness, pathogenicity, and affinity for the trachea and kidneys among each other and with their parental viruses; however, some of them showed varying biological characteristics. Point mutations observed in the receptor-binding domain and hypervariable region of the S1 subunit of the spike protein likely have an effect on these differences in biological characteristics, although the influence of other factors-such as host innate-immune responses and changes in genomic regions beyond the S1 protein-might also be responsible for such changes.

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Section: Discussionmentioning

confidence: 87%

Genetic and biological characteristics of four novel recombinant avian infectious bronchitis viruses isolated in China

Ren

Sheng

et al. 2019

Virus Research

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“…Recombination is involved in the emergence and evolution of IBV or can even directly lead to the emergence of new coronaviruses and related diseases [36] Recombination can occur between field isolates or between field and vaccine viruses [36,37,38]. In our study, convincing evidence showed five S1-gene recombinants GX-NN8, GX-NN9, GX-NN10, GX-NN11 and GX-YL7, with their putative parental strains of vaccine strain 4/91 and CK/CH/LSC/99I-Type field strain GX-YL2, and their crossover regions were at nucleotide position 7–677 or 7–678.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Major Structural Protein Genes of Avian Coronavirus Infectious Bronchitis Virus Isolates in Southern China

Huang

et al. 2013

Viruses

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To gain comprehensive genetic information of circulating avian coronavirus infectious bronchitis virus (IBV) isolates in China, analysis of the phylogenetic tree, entropy of the amino acid sequences, and the positive selection as well as computational recombinations of S1, M and N genes of 23 IBV isolates was conducted in the present study. The phylogenetic trees based on the S1, M and N genes exhibited considerably different topology and the CK/CH/LSC/99I-type isolates were the predominant IBVs based on the phylogenetic analysis of S1 gene. Results of entropy of amino acid sequences revealed that the S1 gene had the largest variation; the M gene had less variation than the N gene. Positive selections were detected in not only S1 but also M and N gene proteins. In addition, five S1 gene recombinants between vaccine strain 4/91 and CK/CH/LSC/99I-type field isolate were confirmed. In conclusion, multiple IBV genotypes co-circulated; genetic diversity and positive selections existed in S1, M and N genes; 4/91 vaccine recombinants emerged in China. Our results show that field IBVs in China are continuing to evolve and vaccine strains may have an important role in the appearance of new IBV strains via recombination. In addition, the present study indicates that IBV evolution is driven by both generations of genetic diversity and selection.

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“…D274 was detected in Sweden, England and Russia in 1995, 1996 and 1998, respectively [28]. Several reports have been confirmed D274 presence in Western Europe from 2002 to 2006 [19,29,30]. D274 was not detected in the Middle East countries before 2005, its first report from Jordan [31].…”

Section: Discussionmentioning

confidence: 95%

Genotyping of avian infectious bronchitis virus in Iran: Detection of D274 and changing in the genotypes rate

Ghalyanchilangeroudi

Hosseini

Mehrabadi

et al. 2019

Comparative Immunology, Microbiology and Infectious Diseases

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The coronavirus avian Infectious bronchitis virus (IBV) poses economic threats to poultry farms worldwide, affecting the performance of both meat-type and egg-laying birds. To define the evolution of recent IBVs in Iran, a genetic analysis based on hypervariable nucleotide sequences of S1 gene was carried out. Tracheal swab samples were collected from 170 Broiler flocks during 2017. Ten tracheal swabs from each flock pooled. From a total number of 170 flocks tested, 84.71% found to be positive. Phylogenetic tree analysis revealed the presence of D274 as a first time in Iran. IS/1494/06 was showed to be dominant IBV type circulating in broiler farms with a significantly higher prevalence than other four genotypes. Considering fluctuations in QX-type prevalence in recent years, continuous monitoring is necessary to reduce economic consequences in layer and broiler farms. The findings highlight the importance of using modified vaccination strategies that are adapted to the changing disease scenario.

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Molecular characterization of infectious bronchitis virus isolates from Russia and neighbouring countries: identification of intertypic recombination in the S1 gene

Cited by 25 publications

References 24 publications

Genetic and biological characteristics of four novel recombinant avian infectious bronchitis viruses isolated in China

Genetic and biological characteristics of four novel recombinant avian infectious bronchitis viruses isolated in China

Molecular Characterization of Major Structural Protein Genes of Avian Coronavirus Infectious Bronchitis Virus Isolates in Southern China

Genotyping of avian infectious bronchitis virus in Iran: Detection of D274 and changing in the genotypes rate

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