Complete genome sequence and comparative genomic analysis of Enterococcus faecalis EF-2001, a probiotic bacterium

Panthee, Suresh; Paudel, Atmika; Hamamoto, Hiroshi; Ogasawara, Akihiko Ano; Iwasa, Toshihiro; Blom, Jochen; Sekimizu, Kazuhisa

doi:10.1016/j.ygeno.2021.03.021

Cited by 33 publications

(22 citation statements)

References 36 publications

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“…In the last decade, there has been an increase in drug-resistant Enterococcus faecalis [ 34 ]. Nowadays, many antibiotics do not work against infections caused by these strains.…”

Section: Resultsmentioning

confidence: 99%

“…We chose the bacterium Enterococcus faecalis because from a bacterium that affects only the dental root, lately it has started to be more frequently present in intrahospital infections [ 34 ]. Enterococcus faecalis (Enterococci family) is an important cause of nosocomial infections and intrahospital infections, due to vancomycin resistant Enterococci (VRE) occurring frequently.…”

Section: Introductionmentioning

confidence: 99%

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Characterization and Antitumoral Activity of Biohybrids Based on Turmeric and Silver/Silver Chloride Nanoparticles

et al. 2021

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The phyto-development of nanomaterials is one of the main challenges for scientists today, as it offers unusual properties and multifunctionality. The originality of our paper lies in the study of new materials based on biomimicking lipid bilayers loaded with chlorophyll, chitosan, and turmeric-generated nano-silver/silver chloride particles. These materials showed a good free radical scavenging capacity between 76.25 and 93.26% (in vitro tested through chemiluminescence method) and a good antimicrobial activity against Enterococcus faecalis bacterium (IZ > 10 mm). The anticancer activity of our developed bio-based materials was investigated against two cancer cell lines (human colorectal adenocarcinoma cells HT-29, and human liver carcinoma cells HepG2) and compared to one healthy cell line (human fibroblast BJ cell line). Cell viability was evaluated for all prepared materials after a 24 h treatment and was used to select the biohybrid with the highest therapeutic index (TI); additionally, the hemolytic activity of the samples was also evaluated. Finally, we investigated the morphological changes induced by the developed materials against the cell lines studied. Biophysical studies on these materials were done by correlating UV–Vis and FTIR absorption spectroscopy, with XRD, SANS, and SAXS methods, and with information provided by microscopic techniques (AFM, SEM/EDS). In conclusion, these “green” developed hybrid systems are an important alternative in cancer treatment, and against health problems associated with drug-resistant infections.

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“…In the last decade, there has been an increase in drug-resistant Enterococcus faecalis [ 34 ]. Nowadays, many antibiotics do not work against infections caused by these strains.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization and Antitumoral Activity of Biohybrids Based on Turmeric and Silver/Silver Chloride Nanoparticles

et al. 2021

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“…These widespread multiple antibiotic resistance genes had been identified on plasmids before [ 94 ]. The gene efrA harbouring in contigs with a prediction of phage origins were identified in all publicly available E. faecalis genome sequences by Panthee and colleges too, along with a large set of phages in the genomes [ 95 ].…”

Section: Discussionmentioning

confidence: 99%

Mobile Antimicrobial Resistance Genes in Probiotics

et al. 2021

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Even though people worldwide tend to consume probiotic products for their beneficial health effects on a daily basis, recently, concerns were outlined regarding the uptake and potential intestinal colonisation of the bacteria that they carry. These bacteria are capable of executing horizontal gene transfer (HGT) which facilitates the movement of various genes, including antimicrobial resistance genes (ARGs), among the donor and recipient bacterial populations. Within our study, 47 shotgun sequencing datasets deriving from various probiotic samples (isolated strains and metagenomes) were bioinformatically analysed. We detected more than 70 ARGs, out of which rpoB mutants conferring resistance to rifampicin, tet(W/N/W) and potentially extended-spectrum beta-lactamase (ESBL) coding TEM-116 were the most common. Numerous ARGs were associated with integrated mobile genetic elements, plasmids or phages promoting the HGT. Our findings raise clinical and public health concerns as the consumption of probiotic products may lead to the transfer of ARGs to human gut bacteria.

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“…77 The genes efrA, dfrE, lsaA and emeA harbouring in contigs with a prediction of phage origins were identified in all publicly available E. faecalis genome sequences by Panthee and colleges too, along with a large set of phages in the genomes. 78 An important aspect to take into consideration by the interpretation of the ARG occurrence in probiotics is that constituent strains can often naturally be, or rendered multiresistant, so that they can be co-administered with oral antibiotics and reduce gastrointestinal side effects. 79,80 In our study we could not distinguish whether the examined samples contained the ARGs for this purpose or not.…”

Section: Discussionmentioning

confidence: 99%

Mobile antimicrobial resistance genes in probiotics

Tóth

Csabai

Judge

et al. 2021

Preprint

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Even though people around the world tend to consume probiotic products for their beneficial health effects on a daily basis, recently, concerns were outlined regarding the uptake and potential intestinal colonisation of the bacteria that they transfer. These bacteria are capable of executing horizontal gene transfer (HGT) which facilitates the movement of various genes, including antimicrobial resistance genes (ARGs), among the donor and recipient bacterial populations. Within our study, 47 shotgun sequencing datasets deriving from various probiotic samples (isolated strains and metagenomes) were bioinformatically analysed. We detected more than 70 ARGs, out of which rpoB, tet(W/N/W) and potentially extended-spectrum beta-lactamase (ESBL) coding TEM-116 were the most common. Numerous ARGs were associated with integrated mobile genetic elements, plasmids or phages promoting the HGT. Our findings raise clinical and public health concerns as the consumption of probiotic products may lead to the transfer of ARGs to human gut bacteria.

show abstract

Complete genome sequence and comparative genomic analysis of Enterococcus faecalis EF-2001, a probiotic bacterium

Cited by 33 publications

References 36 publications

Characterization and Antitumoral Activity of Biohybrids Based on Turmeric and Silver/Silver Chloride Nanoparticles

Characterization and Antitumoral Activity of Biohybrids Based on Turmeric and Silver/Silver Chloride Nanoparticles

Mobile Antimicrobial Resistance Genes in Probiotics

Mobile antimicrobial resistance genes in probiotics

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