Mobile antimicrobial resistance genes in probiotics

Tóth, Adrienn; Csabai, István; Judge, Maura Fiona; Maróti, Gergely; Becsei, Ágnes; Spisák, Sándor; Solymosi, Norbert

doi:10.1101/2021.05.04.442546

Cited by 12 publications

(9 citation statements)

References 95 publications

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“…and have been applied in studies such asLeech et al (2020), Toth et al (2020), and Toth et al (2021. However, these approaches would rely on robust databases that are representative of the wider microbial and genomic diversity that can occur in foods produced by spontaneous fermentation compared to those produced by starter culture(Leech et al, 2020).…”

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confidence: 99%

Are spontaneously fermented plant‐based foods potential sources of transferable antibiotic resistance genes?

Jasiak

Amund

2021

Food Frontiers

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Spontaneously fermented plant-based foods are highly valued for their unique organoleptic characteristics and presumed health benefits. However, because natural (spontaneous) fermentation does not involve the employment of starter cultures, the safety of spontaneously fermented plant-based products is not easily controlled. This review examines the presence of antibiotic resistance determinants in lactic acid bacteria (LAB) from plant-based, naturally fermented foods. Phenotypic resistance to clinically relevant antibiotics has been widely observed in LAB from a variety of food types.The larger proportion of the resistance traits reported in this review can be considered intrinsic to LAB. However, some of the identified traits, especially resistance genes to tetracycline and erythromycin, have the potential for horizontal spread. Further molecular characterization is required to determine the genetic basis of detected resistance traits that have only been assessed using phenotypic methods. The implementation of novel safety measures in the production of spontaneously fermented plant-based foods should be considered in the future.

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confidence: 99%

Are spontaneously fermented plant‐based foods potential sources of transferable antibiotic resistance genes?

Jasiak

Amund

2021

Food Frontiers

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“…Francisella showed the highest ratio of samples with at least one ARG (16.3%), however, ARG-positivity was generally very low in the rest of the analysed genera (only 2.2 %). This finding is surprising at first, considering the vast amount of antibiotic pollution and ARG prevalence of the natural and artificial environments [3][4][5][6][7][33][34][35] . The effect of natural environments on the spread of antimicrobial resistance (AMR) is well represented by the fact that wild animals were found to harbour different ARGs 36,37 , even though it might correlate with anthropogenic influence 38,39 .…”

Section: Discussionmentioning

confidence: 98%

Antimicrobial resistance gene lack in tick-borne pathogenic bacteria

Papp

Tóth

Valcz

et al. 2022

Preprint

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Tick-borne infections, including those of bacterial origin, are significant public health issues. Antimicrobial resistance (AMR), which is one of the most pressing health challenges of our time, is driven by specific genetic determinants, primarily by the antimicrobial resistance genes (ARGs) of bacteria. In our work, we investigated the occurrence of ARGs in the genomes of tick-borne bacterial species that can cause human infections. For this purpose, we processed short/long reads of 1550 bacterial isolates of the genera Anaplasma (n=20), Bartonella (n=131), Borrelia (n=311), Coxiella (n=73), Ehrlichia (n=13), Francisella (n=959) and Rickettsia (n=43) generated by second/third generation sequencing that have been freely accessible at the NCBI SRA repository. From Francisella tularensis, 98.9% of the samples contained the FTU-1 gene, and 16.3% contained additional ARGs. Only 2.2% of isolates from other genera (Bartonella: 2, Coxiella: 8, Ehrlichia: 1, Rickettsia: 2) contained any ARG. We found that the odds of ARG occurrence in Coxiella samples were significantly higher in isolates related to farm animals than from other sources. Our results describe a lack in ARGs in these bacteria and suggest that antibiotic susceptibility testing might be considered before the treatment of tick-borne infections in farm animals.

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“…Similar investigation findings were reported by Cudkowicz and Schuldiner (2019) in Israel. These genes have been previously described in this species, and many of them as even exclusive to it, according to the CARD database (Toth et al, 2021;Jia et al, 2016;McArthur et al, 2013). Gram-negative bacteria typically display the porins in their outer membrane involved in outer membrane protein A (ompA) that are responsible for reducing the permeability to antibiotics (Smani et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Whole genome sequencing and characteristics of extended-spectrum beta-lactamase producing Escherichia coli isolated from poultry farms in Banaskantha, India

Patel¹,

Pandey

Patel

et al. 2022

Front. Microbiol.

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Worldwide dissemination of extended-spectrum -lactamase (ESBL)-producing Escherichia coli constitutes an emerging global health issue, with animal food products contributing as potential reservoirs. ESBL E. coli infection is associated with the high mortality and mobility rate in developing countries due to less susceptibility to antibiotics. The present study aimed to elucidate the molecular characteristics and sequence-based analysis of ESBL E. coli in the Gujarat state of India. This study included 108 E. coli strains were isolated from different poultry farms (broiler and layer) in the Banaskantha District. PCR was employed to identify genotypic ESBL-producing antimicrobial resistance genes. Overall, a high occurrence of ESBL genes was found in poultry farms due to the high usage of antimicrobials. The PCR analysis revealed that 79.62% of isolates were detected positive with one or more ESBL genes. Among them, blaTEM (63.88%) was found to be the predominant genotype, followed by blaSHV (30.55%) and blaOXA (28.70%). In the blaCTX-M group, a higher occurrence was observed in blaCTX-M-9 (23.14%), followed by blaCTX-M-2 (24.07%) and blaCTX-M-1 (22.22%). We used the whole-genome sequencing (WGS) method to evaluate the antimicrobial resistance genes, virulence factors, single nucleotide polymorphisms (SNPs), plasmid replicons, and plasmid-mediated AMR genes of one ESBL E. coli isolated. We examined the genetic relatedness of a human pathogenic E. coli strain by comparing its sequence with the broad geographical reference E. coli sequences. Escherichia coli ST 681 was determined using multi-locus sequence typing. We compared our findings to the reference sequence of Escherichia coli str. K- 12 substr. MG1655. We found 24,937 SNPs with 21,792 in the genic region, 3,145 in the intergenic region, and six InDels across the genome. The WGS analysis revealed 46 antimicrobial resistance genes and seven plasmid-mediated AMR genes viz., tetA, qnrS1, dfrA14, sul2, aph(3”)-lb, aph(6)-ld, and Aph(3’)-la. The ST 681 was found to have Cib, traT, and terC virulence factors and two plasmid replicons, IncFII(pHN7A8) and IncI1-I(Alpha). This study revealed a higher occurrence of ESBL E. coli detected in poultry.

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Mobile antimicrobial resistance genes in probiotics

Cited by 12 publications

References 95 publications

Are spontaneously fermented plant‐based foods potential sources of transferable antibiotic resistance genes?

Are spontaneously fermented plant‐based foods potential sources of transferable antibiotic resistance genes?

Antimicrobial resistance gene lack in tick-borne pathogenic bacteria

Whole genome sequencing and characteristics of extended-spectrum beta-lactamase producing Escherichia coli isolated from poultry farms in Banaskantha, India

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