Complete Genome Sequence Analysis of Enterobacter sp. SA187, a Plant Multi-Stress Tolerance Promoting Endophytic Bacterium

Abstract

Enterobacter sp. SA187 is an endophytic bacterium that has been isolated from root nodules of the indigenous desert plant Indigofera argentea. SA187 could survive in the rhizosphere as well as in association with different plant species, and was able to provide abiotic stress tolerance to Arabidopsis thaliana. The genome sequence of SA187 was obtained by using Pacific BioScience (PacBio) single-molecule sequencing technology, with average coverage of 275X. The genome of SA187 consists of one single 4,429,597 bp chromosome, with an average 56% GC content and 4,347 predicted protein coding DNA sequences (CDS), 153 ncRNA, 7 rRNA, and 84 tRNA. Functional analysis of the SA187 genome revealed a large number of genes involved in uptake and exchange of nutrients, chemotaxis, mobilization and plant colonization. A high number of genes were also found to be involved in survival, defense against oxidative stress and production of antimicrobial compounds and toxins. Moreover, different metabolic pathways were identified that potentially contribute to plant growth promotion. The information encoded in the genome of SA187 reveals the characteristics of a dualistic lifestyle of a bacterium that can adapt to different environments and promote the growth of plants. This information provides a better understanding of the mechanisms involved in plant-microbe interaction and could be further exploited to develop SA187 as a biological agent to improve agricultural practices in marginal and arid lands.

Genome analysis of a wild rumen bacterium Enterobacter aerogenes LU2 - a novel bio-based succinic acid producer

“…The high percentage of genes belonging to the E and G classes as well as genes involved in the Transport and metabolism of inorganic ions (P) may suggest the innate capacity of the LU2 strain to compete with other microorganisms and survive in the cow rumen. A similar conclusion has been presented by Andrés-Barrao et al 21 , who conducted their study on Enterobacter aerogenes SA187 in reference to the ability of this strain to survive in the rhizosphere as well as in cooperation with many different plant species. Furthermore, it was claimed that SA187 has a beneficial effect on Arabidopsis thaliana by providing abiotic stress tolerance.…”

“…For functional studies of genome sequences, the COG database, which is a phylogenetic classification of encoded proteins, was used, and the distribution of genes within the COG categories is provided in Fig. 2 and Additional file 1 20,21 . A total of 4181 out of 4741 (88,2%) coding sequences were assigned to 20 out of 25 COGs.…”

Genomic Analysis of Bacillus megaterium NCT-2 Reveals Its Genetic Basis for the Bioremediation of Secondary Salinization Soil

“…International Journal of Genomics with their environment adaptability [40]. One typical characteristic of soil microorganisms is the high number of rRNAs, which is helpful for fast growth, successful sporulation, germination, and rapid response to changing the availability of nutrients [41][42][43][44]. These features indicate that strain NCT-2 has great ability of adaptation to various environments.…”

Rhizosphere metagenomics of mine tailings colonizing plants: assembling and selecting synthetic bacterial communities to enhance in situ bioremediation

“…SA187. This bacterial strain was isolated from the root endosphere of a desert plant and showed plant growth promoting activity (Andrés-Barrao et al, 2017). Furthermore, its genome sequence revealed proteins associated with oxidative stress tolerance, antibiotic production, plant hormone regulation, and adhesion (Andrés-Barrao et al, 2017).…”