The emergence of inexpensive and low-power wireless communication hardware and various handheld, wearable, and embedded computing technologies is making computing and communication devices more mobile and ubiquitous. Due to the mobility and high-density of networkenabled devices, short range mobile ad hoc networks (MANET) are instantaneously and autonomously formed to facilitate exchange of information. In MANET, interactions among the devices are driven by constantly changing contextual and environmental conditions, rather than by the applications resident on the devices. This trend makes Autonomous Decentralized Systems (ADS) a desirable architecture for facilitating ad hoc communication among mobile devices. In this paper, Reconfigurable ContextSensitive Middleware (RCSM) is presented to facilitate ADS applications in MANET.
Sparse long-term Asian monsoon (AM) records have limited our ability to understand and accurately model low-frequency AM variability. Here we present a gridded 544-yr (from 1470 to 2013) reconstructed Asian summer precipitation (RAP) dataset by weighted merging of two complementary proxies including 453 tree-ring-width chronologies and 71 historical documentary records. The RAP dataset provides substantially improved data quality when compared with single-proxy-type reconstructions. Skillful reconstructions are obtained in East and North China, northern India and Pakistan, the Indochina Peninsula, midlatitude Asia, the Maritime Continent, and southern Japan. The RAP faithfully illustrates large-scale regional rainfall variability but has more uncertainties in representing small-scale local rainfall anomalies. The RAP reproduces a realistic climatology and captures well the year-to-year rainfall variability averaged over monsoon Asia, arid central Asia, and all of Asia during the twentieth century. It also shows a general agreement with other proxies (speleothems and ice cores) during the period of 1470–1920. The RAP captures the remarkably abrupt change during the 1600s recorded in the upwelling proxy over the Arabian Sea. Four major modes of variability of the Asian summer precipitation are identified with the long record of the RAP, including a biennial El Niño–Southern Oscillation (ENSO) mode, a low-frequency ENSO mode, a central Pacific El Niño–like decadal mode, and an interdecadal mode. In sum, the RAP provides a valuable dataset for study of the large-scale Asian summer precipitation variability, especially the decadal–centennial variability that is caused by external forcing and internal feedback processes within the Earth climate system.
To investigate the molecular mechanisms underlying the adaptation of Bifidobacterium longum to the intestinal tract, we utilized a new model for rabbit intestinal culture of B. longum and reported the changes in proteomic profiles after incubation in the in vivo environment. By 2D-PAGE coupled with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and/or electrospray ionization tandem mass spectrometry (ESI-MS/MS) analyses, proteomic profiles of B. longum strain NCC2705 grown in the in vivo and in vitro environments were compared. Confirmed by semiquantitative RT-PCR, which exhibited at least a 3-fold change or greater, 19 up-regulated proteins, 14 down-regulated proteins, and 4 proteins with mobility changes were identified during intestinal growth. These identified proteins include key stress proteins, metabolism-related proteins, and proteins related to translation. Our results indicate that some useful proteins are expressed at higher levels in cells during intestinal growth. These proteins reflected the adaptation of B. longum NCC2705 to the intestine, such as EF-Tu which contributes to the retention or attachment as a Bifidobacterium adhesin-like factor, bile salt hydrolase (BSH) which might play an important role in the molecular mechanisms for the initial interaction of probiotic with the intestinal environment, and stress proteins which defend B. longum against the action of bile salts and other harmful ingredients of the gastrointestinal tract (GIT). The most striking fact of our observation was that four proteins GlnA1, PurC, LuxS, and Pgk exhibit clear post-translational modification. Western blot (WB) analysis and Pro-Q Diamond staining revealed that substances of the GIT trigger Pgk and LuxS phosphorylation at Ser/Thr residues for bacteria grown in vivo. These proteins were identified for the first time as bifidobacterial phosphoproteins. Our data suggest that the phosphorylated autoinducer-2 production protein LuxS of B. longum NCC2705 (LuxS-P) is the active form of LuxS and that LuxS-P may play a key role in the regulation of quorum sensing.
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