Complete de novo assembly of Wolbachia endosymbiont of Diaphorina citri Kuwayama (Hemiptera: Liviidae) using long-read genome sequencing

Neupane, Surendra; Bonilla, Sylvia; Manalo, Andrew M.; Pelz‐Stelinski, Kirsten S.

doi:10.1038/s41598-021-03184-0

Cited by 17 publications

(15 citation statements)

References 109 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…wOeGib-WalA and Wolbachia sp. wOeGib-WalB share most of their gene content (Figure 3C) [56]. Most accessory gene families in both genomes are hypothetical proteins (Figure 3C; de-novo OGs, COG category S) with close homologs in other Wolbachia suggesting a differential loss in one of the genomes.…”

Section: Wolbachiamentioning

confidence: 99%

One to host them all: genomics of the diverse bacterial endosymbionts of the spiderOedothorax gibbosus

Halter

Köstlbacher

Rattei

et al. 2022

Preprint

View full text Add to dashboard Cite

Bacterial endosymbionts of the groups Wolbachia, Cardinium and Rickettsiaceae are well-known for their diverse effects on their arthropod hosts, ranging from mutualistic relationships to reproductive phenotypes. Here, we analyzed a unique system in which the dwarf spider Oedothorax gibbosus is co-infected with up to five different endosymbionts affiliated with Wolbachia, ‘Candidatus Tisiphia’ (formerly Torix group Rickettsia), Cardinium, and Rhabdochlamydia. Using short-read genome sequencing data, we show that the endosymbionts are heterogeneously distributed among O. gibbosus populations and are frequently found co-infecting spider individuals. To study this intricate host-endosymbiont system on a genome resolved level, we used long-read sequencing to reconstruct closed genomes of the Wolbachia, ‘Ca. Tisiphia’ and Cardinium endosymbionts. We provide insights in the ecology and evolution of the endosymbionts and shed light on the interactions with their spider host. We detected high quantities of transposable elements in all endosymbiont genomes and provide evidence that ancestors of the Cardinium, ‘Ca. Tisiphia’ and Wolbachia endosymbionts have co-infected the same hosts in the past. Our findings contribute to broadening our knowledge about endosymbionts infecting one of the largest animal phyla on earth and show the usefulness of transposable elements as an evolutionary “contact-tracing” tool.Data summaryAll supporting data, code and protocols have been provided within the article or through supplementary data files. Seven supplementary figures and seven supplementary tables are available with the online version of this article. Sequencing data used in this study was generated and previously published by Hendrickx et al., 2021. Genome assemblies generated in this study have been deposited under the project PRJEB52003 at DDBJ/ENA/GenBank. The MAG of R. oedothoracis OV001 was deposited at DDBJ/ENA/GenBank under the sample SAMN28026840. The genome of ‘Candidatus Rhabdochlamydia oedothoracis W744×776’ was previously published by Halter et al., 2022 and is available at DDBJ/ENA/GenBank (accession: CP075587-CP075588). The collection of genomes and proteomes, all files for phylogenetic analyses including gene alignments, concatenated alignments, and tree files, and original output files of the HGT and SNP predictions used in this study are available at zenodo (https://doi.org/10.5281/zenodo.6362846).

show abstract

Section: Wolbachiamentioning

confidence: 99%

One to host them all: genomics of the diverse bacterial endosymbionts of the spiderOedothorax gibbosus

Halter

Köstlbacher

Rattei

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…As previously mentioned, strategies based on the maternally inherited endosymbiont Wolbachia is currently under development for insect borne diseases control by either population replacement or population suppression strategies ( Hoffmann et al., 2011 ; Zheng et al., 2019 ; Crawford et al., 2020 ; Neupane et al., 2022 ). Such disease control approaches are based on the ability of Wolbachia to inhibit the pathogen transmission of insect vectors ( Hedges et al., 2008 ; Moreira et al., 2009 ; Bian et al., 2013 ).…”

Section: Introductionmentioning

confidence: 99%

The phylogeny and distribution of Wolbachia in two pathogen vector insects, Asian citrus psyllid and Longan psyllid

Qiu²,

Su³

et al. 2023

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

BackgroundWolbachia is the most abundant bacterial endosymbiont among insects. It can play a prominent role in the development, reproduction and immunity of its given insect host. To date, Wolbachia presence is well studied within aphids, whiteflies and planthoppers, but relatively few studies have investigated its presence in psyllids.MethodsHere, the infection status of Wolbachia in five species of psyllid, including Asian citrus psyllid Diaphorina citri and longan psyllid Cornegenapsylla sinica was investigated. The phylogenetic relationships of different Wolbachia lines and their infection density and patterns in D. citri and C. sinica from different countries was also examined.ResultsThe infection rates of Wolbachia in D. citri and C. sinica were both 100%, and their sequencing types are ST173 and ST532 respectively. Phylogenetic analysis revealed that the Wolbachia lines in D. citri and C. sinica both belong to the Con subgroup of Wolbachia supergroup B. In addition, Wolbachia displayed a scattered localization pattern in the 5th instar nymphs and in the reproductive organs of both D. citri and C. sinica but differed in other tissues; it was highest in the midgut, lowest in the salivary glands and medium in both the testes and ovaries.ConclusionOur findings assist in further understanding the coevolution of Wolbachia and its psyllid hosts. Given that Wolbachia could play an important role in insect pest control and pathogen transmission inhibition, our findings may also provide new insights for development of control strategies for D. citri and C. sinica.

show abstract

“…High-quality, clean data were mapped onto the reference genome of Desmodesmus sp. using Hisat2 [ 31 ].…”

Section: Methodsmentioning

confidence: 99%

Integrated Omics Approach to Discover Differences in the Metabolism of a New Tibetan Desmodesmus sp. in Two Types of Sewage Treatments

Wang

Chen

Zhang³

et al. 2023

Metabolites

View full text Add to dashboard Cite

Microalgae are now widely applied in municipal (YH_3) and industrial sewage (YH_4) treatments. Through integrated omics analysis, we studied the similarities and differences at the molecular level between the two different types of sewage treatment processes. The most significantly enriched gene ontology (GO) terms in both types of sewage treatments were the ribosome, photosynthesis, and proteasome pathways. The results show that the pathways of differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) were enriched for photosynthesis, glyoxylate and dicarboxylate metabolism, and carbon fixation in photosynthetic organisms. Considering YH_3 vs. YH_4, the metabolism of citrate, sedoheptulose-7P, and succinate was significantly upregulated. In addition, the results showed that the pathways of DEGs and DAMs were enriched in terms of amino acid metabolism and carotenoid biosynthesis in YH_4 vs. YH_3. The metabolism of S-Adenosyl-L-homocysteine was significantly downregulated, 2-oxobutanoate was significantly upregulated and downregulated, and the metabolism of abscisic acid glucose ester (ABA-GE) was also significantly upregulated. Overall, the results of this paper will help to improve the basic knowledge of the molecular response of microalgae to sewage treatments, and help design a response strategy based on microalgae for complex, mixed sewage treatments.

show abstract

Complete de novo assembly of Wolbachia endosymbiont of Diaphorina citri Kuwayama (Hemiptera: Liviidae) using long-read genome sequencing

Cited by 17 publications

References 109 publications

One to host them all: genomics of the diverse bacterial endosymbionts of the spiderOedothorax gibbosus

One to host them all: genomics of the diverse bacterial endosymbionts of the spiderOedothorax gibbosus

The phylogeny and distribution of Wolbachia in two pathogen vector insects, Asian citrus psyllid and Longan psyllid

Integrated Omics Approach to Discover Differences in the Metabolism of a New Tibetan Desmodesmus sp. in Two Types of Sewage Treatments

Contact Info

Product

Resources

About