Complete assessment of whole-body n-3 and n-6 PUFA synthesis-secretion kinetics and DHA turnover in a rodent model

Metherel, Adam H.; Lacombe, R.J. Scott; Chouinard-Watkins, Rapha eumll; Hopperton, Kathryn E.; Bazinet, Richard P.

doi:10.1194/jlr.m081380

Cited by 23 publications

(18 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…EPA and DHA can also be synthesized from ALA [7], a process that involves several steps orchestrated by multiple elongases, desaturases, and β-oxidases [8]. However, the synthesis of EPA from ALA occurs at a low rate in mammals [9]. Of note, the same enzymes are employed by omega-6 fatty acids for their metabolic pathways.…”

Section: Introductionmentioning

confidence: 99%

Effects of Omega-3 Fatty Acids on Immune Cells

Gutiérrez

Svahn

Johansson

2019

IJMS

397

301

View full text Add to dashboard Cite

Alterations on the immune system caused by omega-3 fatty acids have been described for 30 years. This family of polyunsaturated fatty acids exerts major alterations on the activation of cells from both the innate and the adaptive immune system, although the mechanisms for such regulation are diverse. First, as a constitutive part of the cellular membrane, omega-3 fatty acids can regulate cellular membrane properties, such as membrane fluidity or complex assembly in lipid rafts. In recent years, however, a new role for omega-3 fatty acids and their derivatives as signaling molecules has emerged. In this review, we describe the latest findings describing the effects of omega-3 fatty acids on different cells from the immune system and their possible molecular mechanisms.

show abstract

Section: Introductionmentioning

confidence: 99%

Effects of Omega-3 Fatty Acids on Immune Cells

Gutiérrez

Svahn

Johansson

2019

IJMS

397

301

View full text Add to dashboard Cite

show abstract

“…However, in western countries DHA intake from fish consumption is very low compared to the intake of LA (from vegetable oils) and AA (from eggs and meat) [ 11 ]. In addition to the high dietary intake of n -6 fatty acids, the synthesis of AA from LA when this fatty acid is highly consumed is more efficient than the synthesis of DHA from ALA [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

The Impact of Maternal Diet during Pregnancy and Lactation on the Fatty Acid Composition of Erythrocytes and Breast Milk of Chilean Women

et al. 2018

View full text Add to dashboard Cite

Maternal diet during pregnancy is relevant for fatty acid supply during fetal life and lactation. Arachidonic (AA) and docosahexaenoic (DHA) acids are also relevant for the normal growth and development of brain and visual system. AA and DHA provided by the mother to the fetus and infant are directly associated with maternal dietary intake and body stores. Our aim was to evaluate the impact of maternal diet, specially referring to the quality of fatty acid intake, in a sample of Chilean women during last stage of pregnancy and across the lactation period. Fifty healthy pregnant women (age range 20–33 years) were studied from the 6th month of pregnancy and followed until 6th month of lactation period. Diet characteristics were evaluated through food frequency questionnaires. Fatty acids composition of erythrocyte phospholipids and breast milk samples was assessed by gas-liquid chromatography. Overall, women had high saturated fatty acids intake with sufficient intake of mono- and polyunsaturated fatty acids (PUFA). Diet was high in n-6 PUFA and low in n-3 PUFA (mainly DHA), with imbalanced n-6/n-3 PUFA ratio. Erythrocytes and breast milk DHA concentration was significantly reduced during lactation compared to pregnancy, a pattern not observed for AA. We concluded that is necessary to increase the intake of n-3 PUFA during pregnancy and lactation by improving the quality of consumed foods with particular emphasis on its DHA content.

show abstract

“…Domenichiello and Bazinet et al elaborated and widely applied this approach. Using this method, Domenichiello et al (Domenichiello et al 2014, Domenichiello et al 2016, Metherel et al 2018) estimated whole body synthesis-secretion rates of DHA from circulating ALA to be 1.5 and 0.045 μmol/day in rats consuming a high ALA or a low ALA containing diet, respectively.…”

Section: Introductionmentioning

confidence: 99%

Quantitation of Human Whole‐Body Synthesis‐Secretion Rates of Docosahexaenoic Acid and Eicosapentaenoate Acid from Circulating Unesterified α‐Linolenic Acid at Steady State

Lin

Hibbeln

Domenichiello

et al. 2018

Lipids

Self Cite

View full text Add to dashboard Cite

The rate at which dietary α-linolenic acid (ALA) is desaturated and elongated to its longer-chain n-3 polyunsaturated fatty acid (PUFA) in humans is not agreed upon. In this study, we applied a methodology developed using rodents to investigate the whole-body, presumably hepatic, synthesis-secretion rates of esterified n-3 PUFA from circulating unesterified ALA in 2 healthy overweight women after 10 weeks of low-linoleate diet exposure. During continuous iv infusion of d5-ALA, 17 arterial blood samples were collected from each subject at -10, 0, 10, 20, 40, 60, 80, 100, 120, 150, 180, and 210 min, and at 4, 5, 6, 7, and 8 h after beginning infusion. Plasma esterified d5-n-3 PUFA concentrations were plotted against the infusion time and fit to a sigmoidal curve using nonlinear regression. These curves were used to estimate kinetic parameters using a kinetic analysis developed using rodents. Calculated synthesis-secretion rates of esterified eicosapentaenoate, n-3 docosapentaenoate, docosahexaenoic acid, tetracosapentaenate, and tetracosahexaenoate from circulating unesterified ALA were 2.1 and 2.7; 1.7 and 5.3; 0.47 and 0.27; 0.30 and 0.30; and 0.32 and 0.27 mg/day for subjects S01 and S02, respectively. This study provides new estimates of whole-body synthesis-secretion rates of esterified longer-chain n-3 PUFA from circulating unesterified ALA in human subjects. This method now can be extended to study factors that regulate human whole-body PUFA synthesis-secretion in health and disease.

show abstract

Complete assessment of whole-body n-3 and n-6 PUFA synthesis-secretion kinetics and DHA turnover in a rodent model

Cited by 23 publications

References 31 publications

Effects of Omega-3 Fatty Acids on Immune Cells

Effects of Omega-3 Fatty Acids on Immune Cells

The Impact of Maternal Diet during Pregnancy and Lactation on the Fatty Acid Composition of Erythrocytes and Breast Milk of Chilean Women

Quantitation of Human Whole‐Body Synthesis‐Secretion Rates of Docosahexaenoic Acid and Eicosapentaenoate Acid from Circulating Unesterified α‐Linolenic Acid at Steady State

Contact Info

Product

Resources

About