Complementary techniques to analyse pericellular matrix formation by human MSC within hyaluronic acid hydrogels

Abstract

Hydrogels are widely used as mimics of the native extracellular matrix as their physical and biological properties can be tuned over a wide range to match those of the native...

GSK3 Inhibitor-Induced Dentinogenesis Using a Hydrogel

“…Hydrogels are increasingly being developed for clinical applications that require drug or cell delivery, as they are biocompatible, and their properties can be tuned for specific applications ( Foyt et al 2018 ). We have previously reported on MA-HA hydrogels for maxillofacial applications ( Salzlechner, Haghighi, et al 2020 ; Salzlechner, Walther, et al 2020 ). These materials are synthesized using an aqueous route, rendering them nontoxic ( Renard et al 1994 ).…”

“…To deliver this drug, we used a hyaluronic acid–based hydrogel ( Fig. 1A-II ), which ( Salzlechner, Haghighi, et al 2020 ; Salzlechner, Walther, et al 2020 ) allows for the incorporation of bioactive drugs and is suitable for direct placement by syringe in clinical contexts ( Fig. 1B-I and B-II ).…”

Designing functional hyaluronic acid-based hydrogels for cartilage tissue engineering

“… Hydrogels Components Physicochemical properties Biofunctions Ref. HA-Tyr HA, tyramine Controllable crosslinking degree; bioadhesive; delivering cells and bioactive molecules Promoting formation of hyaline cartilage (lower crosslinked) and fibrocartilage (higher crosslinked); anti-inflammatory ability; enhancing ECM deposition; accelerating cartilage repair [ [109] , [110] , [111] , [112] , [113] ] HA-Tyr/SF HA, tyramine, silk fibroin Adjustable mechanical properties; sustained release of the drug Promoting chondrogenic marker genes expression and ECM deposition [ 116 , 118 ] HA-Tyr/Gel-Tyr HA, tyramine, gelatin Suitable mechanical properties; electrical conductivity Enhancing the chondrogenic differentiation of BMSCs under electrical stimulation [ 119 ] HA-Dopa HA, dopamine Enhanced adhesion Reducing cartilage friction and wear to protect cartilage [ 126 ] d-AHA-Dopa Dialdehyde HA, dopamine Higher tissue bonding strength than HA-Dopa; rapid gel formation Good cytocompatibility [ 127 ] HA-MA-Dopa HA, methacrylic anhydride, dopamine Tissue adhesion; enhanced cell-tissue interaction Promoting chondrogenesis of hMSCs and cartilage-like matrix formation [ 128 , 129 ] HA-MA-Dopa/Fe 3+ HA, methacrylic anhydride, dopamine, Fe 3+ Increasing mechanical strength and rapid self-healing performance As a soft and tough scaffold in the replacement of biological tissues [ 130 ] HA-furan-Dopa/HA-furan-PBA HA, furfurylamine, dopamine, phenylboronic acid pH responsiveness; adhesion; anti-degradation and superior mechanical properties Maintaining the viability and proliferation of cells; reducing the cell death caused by dopamine oxidation …”

Growing Pains: The Need for Engineered Platforms to Study Growth Plate Biology

“…Further expanding on this nascent protein labeling technique, Salzlechner et al combined the fluorescent labeling of nascent proteins with Raman spectroscopy to look at the biochemical content of cells in addition to exploring the content of the pericellular environment. [ 198 ] Raman spectroscopy is a nondestructive, noninvasive spectroscopic technique to obtain detailed information about cells and their biochemical properties. Raman spectroscopy has been used to evaluate chondrocyte differentiation and the extracellular matrix proteins present in cartilage, [ 67,199 ] to determine the strength and mineral quality of bone, [ 200 ] and to grade chondrogenic and osteosarcoma tumors.…”