Complementarity in Phenolic Compounds and the Antioxidant Activities of Phaseolus coccineus L. and P. vulgaris L. Landraces

Abstract

Phaseolus vulgaris L. is one of the most consumed and documented legumes in regard to its grain composition, but little is known about P. coccineus L. To evaluate and compare the phenolic compound content and antioxidant activity between landraces of P. coccineus and P. vulgaris, a total of 14 accessions of P. coccineus and P. vulgaris were collected from farmers in Oaxaca, Mexico. Based on reference standards and spectrophotometry, the polyphenol, flavonoid and anthocyanin contents were quantified, and the antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The results showed significant differences (p ≤ 0.05) between species and accessions, where P. coccineus and P. vulgaris significantly differed in their contents of polyphenols, flavonoids, and anthocyanins, as well as their antioxidant activity in the seed coat and cotyledons. Higher concentrations were found in the seed coat than in the cotyledons for both species. P. vulgaris had a higher anthocyanin content in the seed coat and a higher flavonoid content in the cotyledons than P. coccineus, but it did not for the other compounds tested. There was high variability among the accessions that were classified into four phenotypic groups: Two of P. coccineus, one of a P. coccineus–vulgaris mixed group, and one group of P. vulgaris.

Bioactive Compounds, Antioxidant Activity, and Antinutritional Content of Legumes: A Comparison between Four Phaseolus Species

“…The species that showed significant differences were P. coccineus and P. polyanthus with P. lunatus, and with P. vulgaris (Figure 3). Similar results were reported when P. coccineus accessions were compared with P. vulgaris accessions, where a higher concentration of polyphenols, flavonoids, anthocyanins, and even higher antioxidant activity was found in the accessions exhibiting purple, black, and brown colors [18,21].…”

Uncovering Phenotypic Diversity and DArTseq Marker Loci Associated with Antioxidant Activity in Common Bean

“…In this study, mean antioxidant activity was found much higher in colored seeds as compared to the white ones. These results are in line with the previous studies stating the existence of higher antioxidant activity in colored seeds as compared to white seeds [58][59][60]. Pearson's correlation coefficient showed a highly significant correlation between colored seeds (brown, beige) and antioxidant activity.…”

Bioactive Compounds of Runner Bean (Phaseolus coccineus L.)

“…Alvarado-López et al [6] reported the presence of anthocyanins in black, purple, and brown Mexican pigment ayocote beans (108.1, 119.3, and 27.89 mgCGE 100 g À1 ), respectively. Capistran et al [20] in a similar study of pigmented P. coccineus landraces from México presented a variation of 15.3 to 137 mg CGE 100 g À1 . Quiroz-Sodi et al [56] studied the seeds of three native bean varieties (P. coccineus) from Querétaro, Mexico.…”

“…The differences, among seed length, width, and thickness, are due to characteristics inherent to the species and are influenced by agroecological factors, cultivation practices, and places of origin [17]. In general, P. coccineus had significantly higher seed length, width, and weight, than other species of Phaseolus [18][19][20] According to the hundred kernel weight values, the P. coccineus varieties mentioned may be classified as "big-grain" legumes [21]. The proximate composition is referred to the content of major groups of compounds contributing to food mass.…”

“…Ja's from Poland of 6.5 mg g À1 seed; in another research on ayocote negro, the value reported was 17.7 mg g À1 [56]. The total phenolic content of different varieties of P. coccineus has been reported with variations from 129.21 to 207.91 mgGAE100 g À1 from Mexican ayocote [6], 808 to 858 mg GAE100 g À1 [20]; in a similar study of pigmented P. coccineus, landraces from Mexico presented a variation of 81 to 197 mgGAE100 g À1 ; Gan et al [61] reported values of mgGAE100 g À1 small runner bean from China. Wołosiak et al [60] report a value of 162 mgGAE100 g À1 on P. coccineus cv.…”