Complement, but Not Platelets, Plays a Pivotal Role in the Outcome of Mucormycosis In Vivo

Harpf, Verena; Rambach, Günter; Parth, Nadia; Neurauter, Magdalena; Fleischer, Verena; Lackner, Michaela; Lass‐Flörl, Cornelia; Würzner, Reinhard; Speth, Cornelia

doi:10.3390/jof9020162

Cited by 3 publications

(1 citation statement)

References 51 publications

(78 reference statements)

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“…Similar to the increased susceptibility to mucormycosis observed when macrophages are depleted, neutropenia is also a risk factor associated with this disease, highlighting the pivotal role of neutrophils [ 79 , 80 ]. Accordingly, the in vivo studies developed in zebrafish larvae showed that the formation of the primary granulomas is executed by neutrophils [ 81 ].…”

Section: Genetic Manipulation In Mucorales and New...mentioning

confidence: 99%

Mucorales and Mucormycosis: Recent Insights and Future Prospects

Tahiri

Lax

Cánovas-Márquez

et al. 2023

JoF

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The classification of Mucorales encompasses a collection of basal fungi that have traditionally demonstrated an aversion to modern genetic manipulation techniques. This aversion led to a scarcity of knowledge regarding their biology compared to other fungal groups. However, the emergence of mucormycosis, a fungal disease caused by Mucorales, has attracted the attention of the clinical field, mainly because available therapies are ineffective for decreasing the fatal outcome associated with the disease. This revitalized curiosity about Mucorales and mucormycosis, also encouraged by the recent COVID-19 pandemic, has spurred a significant and productive effort to uncover their mysteries in recent years. Here, we elaborate on the most remarkable breakthroughs related to the recently discovered genetic advances in Mucorales and mucormycosis. The utilization of a few genetic study models has enabled the identification of virulence factors in Mucorales that were previously described in other pathogens. More notably, recent investigations have identified novel genes and mechanisms controlling the pathogenic potential of Mucorales and their interactions with the host, providing fresh avenues to devise new strategies against mucormycosis. Finally, new study models are allowing virulence studies that were previously hampered in Mucorales, predicting a prolific future for the field.

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Section: Genetic Manipulation In Mucorales and New...mentioning

confidence: 99%

Mucorales and Mucormycosis: Recent Insights and Future Prospects

Tahiri

Lax

Cánovas-Márquez

et al. 2023

JoF

View full text Add to dashboard Cite

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Review on Mucormycosis: Pathogenesis, Epidemiology, Microbiology and Diagnosis

Sharma,

Alam,

Dhoundiyal

et al. 2024

IDDT

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Mucormycosis is a serious and invasive fungal infection caused by Mucorales fungi. This review article provides a concise overview of the pathogenesis, epidemiology, microbiology, and diagnosis of mucormycosis. The introduction section highlights the key microbiological properties of the pathogen and delves into the underlying mechanisms of mucormycosis pathogenesis, including the invasion and proliferation of the fungus within the host. The description of the disease section focuses on the epidemiology of mucormycosis, including its incidence, risk factors, and geographical distribution. It also explores the specific context of mucormycosis infection about COVID-19 and diabetes mellitus, highlighting the increased susceptibility observed in individuals with these conditions. A case study illustrates the clinical manifestations and challenges associated with mucormycosis, emphasizing the importance of early detection. Additionally, the review discusses the diagnosis of mucormycosis, emphasizing the significance of clinical assessment, radiological imaging, and microbiological tests for accurate and timely detection of the infection. Regarding treatment, the article covers the various therapeutic approaches, including antifungal therapy, surgical interventions, and management of underlying predisposing conditions. The limitations and challenges associated with treatment options are also addressed. This review aims to provide a comprehensive understanding of mucormycosis, equipping healthcare professionals with valuable insights into its pathogenesis, epidemiology, microbiology, and diagnostic strategies. By enhancing knowledge and awareness of this fungal infection, this review can improve patient outcomes through early diagnosis and appropriate management.

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Complement Component C5a and Fungal Pathogen Induce Diverse Responses through Crosstalk between Transient Receptor Potential Channel (TRPs) Subtypes in Human Conjunctival Epithelial Cells

Rech,

Dietrich-Ntoukas,

Reinach

et al. 2024

Cells

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The conjunctiva has immune-responsive properties to protect the eye from infections. Its innate immune system reacts against external pathogens, such as fungi. The complement factor C5a is an important contributor to the initial immune response. It is known that activation of transient-receptor-potential-vanilloid 1 (TRPV1) and TRP-melastatin 8 (TRPM8) channels is involved in different immune reactions and inflammation in the human body. The aim of this study was to determine if C5a and mucor racemosus e voluminae cellulae (MR) modulate Ca2+-signaling through changes in TRPs activity in human conjunctival epithelial cells (HCjECs). Furthermore, crosstalk was examined between C5a and MR in mediating calcium regulation. Intracellular Ca2+-concentration ([Ca2+]i) was measured by fluorescence calcium imaging, and whole-cell currents were recorded using the planar-patch-clamp technique. MR was used as a purified extract. Application of C5a (0.05–50 ng/mL) increased both [Ca2+]i and whole-cell currents, which were suppressed by either the TRPV1-blocker AMG 9810 or the TRPM8-blocker AMTB (both 20 µM). The N-terminal peptide C5L2p (20–50 ng/mL) blocked rises in [Ca2+]i induced by C5a. Moreover, the MR-induced rise in Ca2+-influx was suppressed by AMG 9810 and AMTB, as well as 0.05 ng/mL C5a. In conclusion, crosstalk between C5a and MR controls human conjunctival cell function through modulating interactions between TRPV1 and TRPM8 channel activity.

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Complement, but Not Platelets, Plays a Pivotal Role in the Outcome of Mucormycosis In Vivo

Cited by 3 publications

References 51 publications

Mucorales and Mucormycosis: Recent Insights and Future Prospects

Mucorales and Mucormycosis: Recent Insights and Future Prospects

Review on Mucormycosis: Pathogenesis, Epidemiology, Microbiology and Diagnosis

Complement Component C5a and Fungal Pathogen Induce Diverse Responses through Crosstalk between Transient Receptor Potential Channel (TRPs) Subtypes in Human Conjunctival Epithelial Cells

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