Complement Activation by Pulp Capping Materials Plays a Significant Role in Both Inflammatory and Pulp Stem Cells' Recruitment

Giraud, Thomas; Rufas, Pierre; Chmilewsky, Fanny; Rombouts, Caroline; Déjou, Jacques; Jeanneau, Charlotte; About, Imad

doi:10.1016/j.joen.2017.02.016

Cited by 25 publications

(22 citation statements)

References 36 publications

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“…5B). Investigating DPSC migration in Boyden chamber also demonstrated a significantly higher migration of these cells in the presence of Biodentine TM as compared to TheraCal ® [54] (Fig. 5B).…”

Section: Pulp Capping Materials Modulate the Pulp Regeneration Potentialmentioning

confidence: 74%

Pulp capping materials modulate the balance between inflammation and regeneration

et al. 2019

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The interrelations between inflammation and regeneration are of particular significance within the dental pulp tissue inextensible environment. Recent data have demonstrated the pulp capacity to respond to insults by initiating an inflammatory reaction and dentin pulp regeneration. Different study models have been developed in vitro and in vivo to investigate the initial steps of pulp inflammation and regeneration. These include endothelial cell interaction with inflammatory cells, stem cell interaction with pulp fibroblasts, migration chambers to study cell recruitment and entire human tooth culture model. Using these models, the pulp has been shown to possess an inherent anti-inflammatory potential and a high regeneration capacity in all teeth and at all ages. The same models were used to investigate the effects of tricalcium silicate-based pulp capping materials, which were found to modulate the pulp anti-inflammatory potential and regeneration capacity. Among these, resin-containing materials such as TheraCal ® shift the pulp response towards the inflammatory reaction while altering the regeneration process. On the opposite, resin-free materials such as Biodentine TM have an anti-inflammatory potential and induce the pulp regeneration capacity. This knowledge contradicts the new tendency of developing resin-based calcium silicate hybrid materials for direct pulp capping. Additionally, it would allow investigating the modulatory effects of newly released pulp capping materials on the balance between tissue inflammation and regeneration. It would also set the basis for developing future capping materials targeting these processes.

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Section: Pulp Capping Materials Modulate the Pulp Regeneration Potentialmentioning

confidence: 74%

Pulp capping materials modulate the balance between inflammation and regeneration

et al. 2019

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“…In addition, recent data have confirmed the involvement of the complement system in the balance between pulp inflammation and regeneration through secretion of active molecules such as C3a, C5a, and membrane attack complex within this inextensible environment (10)(11)(12). Furthermore, it has been shown that pulp-capping material can modulate C5a secretion (13).…”

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confidence: 84%

“…The materials were prepared according to manufacturers' instructions and inserted into standardized silicone molds. Samples of set materials were sterilized with UV (20 minutes on each side) and incubated in serum-free MEM (0.05 cm 2 /mL) for 24 hours at 37 C, 5% CO 2. as described previously (13). These media will hereafter be called conditioned media.…”

Section: Conditioned Medium Preparationmentioning

confidence: 99%

Tricalcium Silicate Capping Materials Modulate Pulp Healing and Inflammatory Activity In Vitro

Giraud

Jeanneau

Bergmann

et al. 2018

Journal of Endodontics

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“…Complement proteins are known to be synthesized by the liver and inflammatory cells, but a recent study has shown that after lipoteichoic acid (LTA) stimulation, pulp fibroblasts secrete functional complement proteins (3). In addition to their functions during the inflammatory reaction, recent data reported the involvement of complement activation in dentin-pulp regeneration (4). Indeed, the complement C3 protein produced by pulp fibroblasts is cleaved to C3a and C3b.…”

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confidence: 99%

Human Pulp Fibroblast Implication in Phagocytosis via Complement Activation

Fournis

Hadjichristou

Jeanneau

et al. 2019

Journal of Endodontics

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Introduction: Previous works have shown that human pulp fibroblasts synthetize all complement components. Local complement activation in the dental pulp is known to be involved in inflammation and regeneration and also in pathogen destruction through membrane attack complex formation. Bacterial elimination by complement-mediated phagocytosis implies microorganism opsonization with the complement C3b protein, which is recognized by specific phagocytic cell CR1 receptors for subsequent intracellular destruction. This work was designed to find out whether pulp fibroblasts produce C3b and check its subsequent implication in bacteria phagocytosis. Methods: The expression of C3b was investigated in carious and healthy human pulp tissues. To simulate a bacterial infection in vitro, cultured human pulp fibroblasts were stimulated with lipoteichoic acid, and C3b secretion was quantified by an enzyme-linked immunosorbent assay. C3b fixation on bacteria (opsonization) and the inflammatory THP-1 cell complement receptor 1 was studied by immunofluorescence. A gentamycin protection assay was used to check the implication of C3b secretion by fibroblasts in bacteria phagocytosis. Results: Pulp cells constitutively express C3b in vivo, and cultured pulp fibroblasts produce C3b. We observed a fixation of this C3b protein on the bacterial surface (opsonization) and the THP-1 CR1 receptor. This recognition leads to a significant increase in bacteria phagocytosis. Conclusions: These results showed that pulp fibroblasts mediate the process of phagocytosis by producing the complement C3b protein and opsonizing bacteria. This highlights a significant role of fibroblasts in the dental pulp local regulation of inflammation.

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Complement Activation by Pulp Capping Materials Plays a Significant Role in Both Inflammatory and Pulp Stem Cells' Recruitment

Cited by 25 publications

References 36 publications

Pulp capping materials modulate the balance between inflammation and regeneration

Pulp capping materials modulate the balance between inflammation and regeneration

Tricalcium Silicate Capping Materials Modulate Pulp Healing and Inflammatory Activity In Vitro

Human Pulp Fibroblast Implication in Phagocytosis via Complement Activation

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