Tricalcium Silicate Capping Materials Modulate Pulp Healing and Inflammatory Activity In Vitro

Giraud, Thomas; Jeanneau, Charlotte; Bergmann, Madison; Laurent, Patrick; About, Imad

doi:10.1016/j.joen.2018.06.009

Cited by 64 publications

(63 citation statements)

References 34 publications

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“…Our results are consistent with other studies that have shown the migration capacity of the root repair materials. For example, iRoot FS (Innovative Bioceramix, Vancouver, BC, Canada) showed cell migration rates higher than ProRootMTA (Dentsply, Tulsa, OK, USA) [28], or Biodentine (Septodont, St Maur des Fosses, France) has been evidenced by anti-inflammatory properties and favored fibroblast migration to the injury site [31]. Nevertheless, the detailed mechanism of how premixed and putty calcium silicate materials favors cell migration is unknown.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Effect of Putty Calcium Silicate Materials on Human Periodontal Ligament Stem Cells

et al. 2020

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New bioactive materials have been developed for retrograde root filling. These materials come into contact with vital tissues and facilitate biomineralization and apical repair. The objective of this study was to evaluate the cytocompatibility and bioactivity of two bioactive cements, Bio-C Repair (Angelus, Londrina, Pr, Brazil) and TotalFill BC RRM putty (FGK, Dentaire SA, La-Chaux-de-fonds, Switzerland). The biological properties in human periodontal ligament stem cells (hPDLSCs) that were exposed to Bio-C Repair and TotalFill BC RRM putty were studied. Cell viability, migration, and cell adhesion were analyzed. Moreover, qPCR and mineralization assay were performed to evaluate the bioactivity potential of these cements. The results were statistically analyzed using ANOVA and the Tukey test (p < 0.05). It was observed that cell viability and cell migration in Bio-C Repair and TotalFill BC RRM putty were similar to the control without statistically significant differences, except at 72 h when TotalFill BC RRM putty was slightly lower (p < 0.05). Excellent cell adhesion and morphology were observed with both Bio-C Repair and TotalFill BC RRM putty. Both cements promoted the osteo- and cementogenic differentiation of hPDLSCs. These results suggest that Bio-C Repair and TotalFill BC RRM putty are biologically appropriate materials to be used as retrograde obturation material.

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Section: Discussionmentioning

confidence: 99%

In Vitro Effect of Putty Calcium Silicate Materials on Human Periodontal Ligament Stem Cells

et al. 2020

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“…Moreover, the resin in pulp capping materials such as ACTIVA BioACTIVE BASE/LINER would presumably shift the regeneration-inflammation balance towards the latter. As in the other light-cured tricalcium silicate, incomplete resin photopolymerization may lead to free monomers' release, and consequently to pulpal toxicity [137]. However, to accurately assess ACTIVA BioACTIVE's reparative potential or influence on the vital pulp in pulp capping procedures, further in vitro and in vivo studies are necessary.…”

Section: Activa Bioactivementioning

confidence: 99%

Bio-Inductive Materials in Direct and Indirect Pulp Capping—A Review Article

2020

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The article is aimed at analyzing the available research and comparing the properties of bio-inductive materials in direct and indirect pulp capping procedures. The properties and clinical performances of four calcium-silicate cements (ProRoot MTA, MTA Angelus, RetroMTA, Biodentine), a light-cured calcium silicate-based material (TheraCal LC) and an enhanced resin-modified glass-ionomer (ACTIVA BioACTIVE) are widely discussed. A correlation of in vitro and in vivo data revealed that, currently, the most validated material for pulp capping procedures is still MTA. Despite Biodentine's superiority in relatively easier manipulation, competitive pricing and predictable clinical outcome, more long-term clinical studies on Biodentine as a pulp capping agent are needed. According to available research, there is also insufficient evidence to support the use of TheraCal LC or ACTIVA BioACTIVE BASE/LINER in vital pulp therapy.

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“…It is proved that calcium-silicate cements used for direct pulp capping affect the the form of direct pulp capping [1][2][3]. It is proved that calcium-silicate cements used for direct pulp capping affect the initial inflammatory stages and induce the healing process [4]. The currently used MTA is considered the golden standard in the biological treatment of the pulp [5].…”

Section: Introductionmentioning

confidence: 99%

“…Under physiological conditions, macrophages are present in the pulp in a dormant state. Only during the development of the inflammatory process, do they show mobility and move to the site of inflammation [3][4][5]9].…”

Section: Introductionmentioning

confidence: 99%

“…One such side effect is an immune response that can lead to increased inflammation. Many studies show that use of biomaterials may cause the release of cytokines and chemokines, along with activation of the complement system, thereby increasing the intensity of inflammation which may significantly shorten the life of the tooth in the oral cavity and, in extreme cases, lead to the necessity of its premature removal [4,5,7,8].…”

Section: Introductionmentioning

confidence: 99%

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Analysis of the Activity and Expression of Cyclooxygenases COX1 and COX2 in THP-1 Monocytes and Macrophages Cultured with BiodentineTM Silicate Cement

Barczak

Palczewska-Komsa

Nowicka

et al. 2020

IJMS

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BiodentineTM is a material based on hydrated calcium silicate with odontotropic properties. However, from the clinician’s perspective, every material used to fill a tooth—even those showing the optimal biochemical parameters—is in fact a foreign body introduced to the organism of the host. Therefore, apart from the chemical parameters of such materials, equally important is the so-called biocompatibility of such materials. The aim of the study was to investigate whether BiodentineTM, used in the regeneration of the pulp-dentine complex, may affect the expression of the enzymes cyclooxygenase 1 (COX1) and cyclooxygenase 2 (COX2) in THP-1 monocytes/macrophages and the amount of prostanoids synthesized by these enzymes-precursors of biologically active prostanoids such as prostaglandin E2 (PGE2) and thromboxane (TXB2) which are mediators of inflammation. An original aspect of this research is the use of the THP-1 monocyte/macrophage cell model and the use of biomaterial in direct contact with cells. In this way we tried to reflect the clinical conditions of regenerative pulp and periodontal tissue treatment using BiodentineTM. The results of our study showed a lack of macrophage activation (measured by flow cytometry) and a lack of stimulation of the expression of the studied cyclooxygenase enzymes (measured by Western blotting and fluorescent microscopy), as well as a lack of increase in the concentration (measured by ELISA method) of their inflammatory mediators (PGE2 and TXB2) in vitro incubated with BiodentineTM.

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Tricalcium Silicate Capping Materials Modulate Pulp Healing and Inflammatory Activity In Vitro

Cited by 64 publications

References 34 publications

In Vitro Effect of Putty Calcium Silicate Materials on Human Periodontal Ligament Stem Cells

In Vitro Effect of Putty Calcium Silicate Materials on Human Periodontal Ligament Stem Cells

Bio-Inductive Materials in Direct and Indirect Pulp Capping—A Review Article

Analysis of the Activity and Expression of Cyclooxygenases COX1 and COX2 in THP-1 Monocytes and Macrophages Cultured with BiodentineTM Silicate Cement

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