Competitive method-based electrochemiluminescent assay with protein–nucleotide conversion for ratio detection to efficiently monitor the drug resistance of cancer cells

Liang, Wenbin; Yang, Mingzhen; Zhuo, Ying; Zheng, Yingning; Xiong, Chengyi; Yuan, Ruo

doi:10.1039/c6sc02801b

Cited by 27 publications

(23 citation statements)

References 25 publications

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“…The ECL process is optical radiation from energy relaxation of luminophore excited species generated during the electrochemical reaction . Due to the advantages of accurate control of the applied potential, quasi-zero background, and cost-effective instrumental setup, ECL technology has been widely applied in the sensing and imaging fields. − The ECL behavior of nanoparticles such as Au nanoclusters and quantum dots has been well studied. − Especially, the acceleration effect of nanoparticles has made great efforts in ECL applications, , for example, the catalytic effect of ZnO/Ce-doped ZnO nanoparticles could induce highly enhanced ECL signal and has been applied in efficient ECL biosensors. , Recently, advances in ECL microscopy for imaging electrochemical reactions on nanoparticles have drawn great attention. Electrocatalytic behaviors of Au–Pt Janus nanoparticles and CdSeTe quantum dots as well as the electrochemistry of particle collision have been imaged and studied via ECL microscopy. ,, However, imaging electrocatalytic activity up to the subparticle level and direct investigation on different crystal facet catalysis at ambient conditions have not been achieved.…”

mentioning

confidence: 99%

In Situ Imaging Facet-Induced Spatial Heterogeneity of Electrocatalytic Reaction Activity at the Subparticle Level via Electrochemiluminescence Microscopy

Chen

Zhao

et al. 2019

Anal. Chem.

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Investigating catalytic behavior of heterogeneous catalysts, especially at the crystal facets level, is crucial for rational catalyst design in the energy and environmental fields. Here we demonstrate an efficient approach to in situ visualize and analyze the heterogeneity of electrocatalytic activity on different facets at the subparticle level via electrochemiluminescence (ECL) microscopy. ZnO crystals with various exposed facet proportions were synthesized, and the correlation between their electrocatalytic performance toward luminol analogue degradation and the exposed facets is established. It is clearly imaged that the ZnO (002) facet has superior catalytic performance compared to the ZnO (100) facet, which is supported by theoretical computation and electrochemical experiments as the facet-induced heterogeneity of the catalytic effect on oxygen reduction into the key reactant for ECL. Accordingly, the spatial heterogeneity of electrocatalytic activity at different facets on one particle is visualized for the first time. The realization of subparticle ECL imaging and kinetic analysis could provide a special approach to visualize facet-induced spatial heterogeneity of catalytic behavior and valuable information for the catalysis study and analysis.

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mentioning

confidence: 99%

In Situ Imaging Facet-Induced Spatial Heterogeneity of Electrocatalytic Reaction Activity at the Subparticle Level via Electrochemiluminescence Microscopy

Chen

Zhao

et al. 2019

Anal. Chem.

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“…First, each glassy carbon electrode (GCE) was pretreated with the previous method . Next, the prepared Py-sp 2 c-CON (10 μL) was deposited on the GCE surface and dried at 37 °C to form a uniform film.…”

Section: Experimental Sectionmentioning

confidence: 59%

“…First, each glassy carbon electrode (GCE) was pretreated with the previous method. 42 Next, the prepared Py-sp 2 c-CON (10 μL) was deposited on the GCE surface and dried at 37 °C to form a uniform film. After decorating 7 μL of Pd NPs on the Py-sp 2 c-CON/GCE, the modified GCE was incubated with 10 μL of Fc-H3 (4.0 μM) at 4 °C for 12 h. Then, the Fc-H3/Pd NPs/Py-sp 2 c-CON/GCE was incubated with 10 μL of hexanethiol (HT, 1.0 mM, ethanol) for 1 h to block the nonspecific sites.…”

Section: ■ Experimental Sectionmentioning

confidence: 92%

Highly Stable Covalent Organic Framework Nanosheets as a New Generation of Electrochemiluminescence Emitters for Ultrasensitive MicroRNA Detection

et al. 2021

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A pyrene-based sp2 carbon-conjugated covalent organic framework (COF) nanosheet (Py-sp2c-CON) with strong and stable electrochemiluminescence (ECL) emission was constructed by CC polycondensation of tetrakis(4-formylphenyl)pyrene (TFPPy) and 2,2′-(1,4-phenylene)diacetonitrile, which was employed as a highly efficient ECL emitter to fabricate an ECL biosensor for the first time. The Py-sp2c-CON exhibited higher ECL intensity and efficiency than those of TFPPy, bulk Py-sp2c-COF, and imine-linked pyrene COF, not only because the pyrene luminophores and aggregation-induced emissive luminogens (cyano-substituted phenylenevinylene) were topologically linked into Py-sp2c-CON, which greatly increased the immobilization amount of luminophores and decreased the aggregation-caused quenching effect and nonradiative transition but also because the porous ultrathin structure of Py-sp2c-CON effectively shortened transport distances of an electron, ion, and co-reactant (S2O8 2–), which made more ECL luminophores be activated and thus efficiently increased the utilization ratio of luminophores. More interestingly, when Bu4NPF6 was introduced into the Py-sp2c-CON/S2O8 2– system as a co-reaction accelerator, the ECL signal of Py-sp2c-CON was further amplified. As expected, the average ECL intensity of the Py-sp2c-CON/S2O8 2–/Bu4NPF6 system was about 2.03, 5.76, 24.31, and 190.33-fold higher than those of Py-sp2c-CON/S2O8 2–, Py-sp2c-COF/S2O8 2–, TFPPy/S2O8 2,– and imine-linked pyrene COF/S2O8 2– systems. Considering these advantages, the Py-sp2c-CON/S2O8 2–/Bu4NPF6 system was employed to prepare an ECL biosensor for microRNA-21 detection, which exhibited a broad linear response (100 aM to 1 nM) and a low detection limit (46 aM). Overall, this work demonstrated that sp2 carbon CONs can be directly used as a high-performance ECL emitter, thus expanding the application scope of COFs and opening a new horizon to develop new types of ECL emitters.

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“…The RCA and self-synthesized nucleotide dendrimer were considered to be efficient strategies to improve analysis performance. Liang et al 80 integrated RCA strategy and enzyme reaction to efficiently estimate the concentration ratio of two proteins (p-glycoprotein and glyceraldehyde 3phosphate dehydrogenase). This sandwich-type ECL immunoassay was based on the conversion of proteins to nucleotide sequences and rolling circle amplification reactions to improve the detection limit and sensitivity.…”

Section: +mentioning

confidence: 99%

Recent Advances in Electrochemical Immunosensors

et al. 2016

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Competitive method-based electrochemiluminescent assay with protein–nucleotide conversion for ratio detection to efficiently monitor the drug resistance of cancer cells

Abstract: A competitive method-based electrochemiluminescent (ECL) assay with a single ECL indicator was proposed to efficiently estimate the concentration ratio of two proteins.

Cited by 27 publications

References 25 publications

In Situ Imaging Facet-Induced Spatial Heterogeneity of Electrocatalytic Reaction Activity at the Subparticle Level via Electrochemiluminescence Microscopy

In Situ Imaging Facet-Induced Spatial Heterogeneity of Electrocatalytic Reaction Activity at the Subparticle Level via Electrochemiluminescence Microscopy

Highly Stable Covalent Organic Framework Nanosheets as a New Generation of Electrochemiluminescence Emitters for Ultrasensitive MicroRNA Detection

Recent Advances in Electrochemical Immunosensors

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