COMPETITIVE ENZYME IMMUNOASSAY FOR THE RAPID IDENTIFICATION OF <i>SALMONELLA</i>

Tsang, Raymond S. W.; Nielsen, Klaus; Balsevicius, S.; Kelly, L.; Khakhria, Rasik; Johnson, Wendy M.

doi:10.1111/j.1745-4581.1995.tb00096.x

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…Since over 95% of natural isolates of Salmonella known to (19,21), it may be expected from the above results that 98% of these pathogens naturally affecting humans may be detected by the T6 capture ELISA, i.e., 99% ϫ 95% of serogroup A to E strains plus 71% ϫ 5% of the higher-serogroup strains.…”

Section: Resultsmentioning

confidence: 89%

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Detection and serogroup differentiation of Salmonella spp. in food within 30 hours by enrichment-immunoassay with a T6 monoclonal antibody capture enzyme-linked immunosorbent assay

Tsui

Roberts

et al. 1996

Appl Environ Microbiol

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We previously described an antigen capture enzyme-linked immunosorbent assay which makes use of monoclonal antibody T6, which recognizes an epitope on the outer core polysaccharide of Salmonella lipopolysaccharide molecules that is common to almost all Salmonella serovars. In this paper, we show that this assay can detect between 10 5 and 10 7 Salmonella cells per ml even in the presence of excess Escherichia coli. A total of 153 of 154 (99%) serogroup A to E strains and 51 of 78 (71%) serogroup F to 67 strains were reactive as determined by this assay. This corresponds to a detection rate of approximately 98% of all salmonellae known to affect humans. None of the 65 strains of non-Salmonella bacteria tested positive. Taking advantage of the O-factor polysaccharides also present on the antigen captured by the immobilized T6 antibody, we showed that 136 of 154 Salmonella serogroup A to E strains (88%) were correctly differentiated according to their serogroups by use of enzyme conjugates of a panel of O-factor-specific monoclonal antibodies. We evaluated this assay for the detection and serogroup differentiation of salmonellae directly from enrichment cultures of simulated food, eggs, pork, and infant formula milk. All 26 samples which had been contaminated with Salmonella spp. were detected by T6 (100% sensitivity), with only one false-positive result from 101 samples not contaminated by Salmonella spp. (99% specificity). The detection time was substantially reduced to between 17 and 29 h, depending on the enrichment methods used. Since there were no false-negative results, we concluded that this enrichment-immunoassay method can afford rapid screening for Salmonella spp. in food samples.

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Section: Resultsmentioning

confidence: 89%

“…Two types of immunoassays using such antibodies have been developed. One is a competitive enzyme immunoassay (15,19), and the other is a capture enzyme-linked immunosorbent assay (ELISA) (4). Both types of assays are capable of specifically detecting a wide range of Salmonella spp.…”

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confidence: 99%