Screening for Salmonella with a Murine Monoclonal Antibody M105 Detects Both Felix O1 Bacteriophage Sensitive and Resistant Salmonella Strains

Tsang, Raymond S. W.; Nielsen, Klaus; Henning, Dale; Poppe, C.; Khakhria, Rasik; Woodward, David L.; Johnson, Wendy M.

doi:10.1016/s0934-8840(97)80071-0

Cited by 6 publications

(9 citation statements)

References 17 publications

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“…However, there was no evidence of cross‐reactivity with these non‐ Salmonella strains in the dot blot assays ( Table 1). Therefore, the dot blot assay results confirmed the genus specificity of the M105 antibody and its potential in a rapid immunological presumptive Salmonella detection method ( Tsang et al . 1991 ; Nielsen et al .…”

Section: Discussionmentioning

confidence: 55%

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The detection of Salmonella using a combined immunomagnetic separation and ELISA end-detection procedure

Mansfield

Forsythe

2000

Lett Appl Microbiol

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The aim of this study was to develop a rapid immunoassay to detect Salmonella bacteria. Skimmed milk powder (SMP) in buffered peptone water was inoculated with six Salmonella strains (Salm. typhimurium, Salm. virchow, Salm. enteritidis, Salm. give, Salm. ealing and Salm. arizonae) at three inoculum levels (about 2–200 cfu 25 g−1 SMP) and incubated (37 °C) overnight. Heat‐treated salmonella cells were immobilized on paramagnetic particles and detected within 3 h using the Salmonella genus‐specific monoclonal antibody M105 in a microtitre plate based assay. The rapid Salmonella detection method combining immunomagnetic separation and ELISA had a total isolation and detection time of less than 24 h, which is significantly shorter than the conventional techniques requiring 72–96 h. The technique had a sensitivity limit of 105−106 cfu ml−1.

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Section: Discussionmentioning

confidence: 55%

“…1987). The antigen is the outer core region of the LPS structure and subsequently needs to be exposed by heat treatment of whole cells ( Tsang et al . 1991 ).…”

Section: Discussionmentioning

confidence: 99%

The detection of Salmonella using a combined immunomagnetic separation and ELISA end-detection procedure

Mansfield

Forsythe

2000

Lett Appl Microbiol

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“…However, reactivities with core-specific monoclonal antibodies (Mabs) and sensitivity to bacteriophages have indicated that there is some subtle diversity in core OS structure in the Salmonellae. [5][6][7][8][9][10] Elucidation of the structure of the sv. Arizonae IIIa core OS 11 provides a structural basis for at least some of these observations.…”

Section: Introductionmentioning

confidence: 99%

Molecular insights into the assembly and diversity of the outer core oligosaccharide in lipopolysaccharides from Escherichia coli and Salmonella

Whitfield

Kaniuk

Frirdich

2003

Journal of Endotoxin Research

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In the Enterobacteriaceae, the core oligosaccharide provides the junction between the highly conserved lipid A and the remarkably diverse polysaccharide O antigen. The basic structure of the inner (lipid A-proximal) core is well conserved, perhaps reflecting constraints imposed by its involvement in the structural integrity of the outer membrane. However, non-stoichiometric modifications do create some structural variants. The outer core may show more variation. Efforts to develop immunoprophylactic strategies based on the core oligosaccharide require a detailed understanding of core immunochemistry, the accessibility of specific epitopes in the LPS, and the distribution of specific structures within natural populations. The availability of sequences for the waa (core biosynthesis) loci and functional data for the gene products provide a molecular basis for the known structural diversity in Escherichia coli and Salmonella core oligosaccharide. Surveys of waa-locus organization have established the distribution of these core types in natural populations and have identified genetic variants that provide candidates for additional novel structures.

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“…Tsang et al . (1991a; 1997) studied the reactivities of different isolates and serotypes of Salmonella with monoclonal antibody (mAb) M105 that recognizes an undefined epitope contained within the three terminal sugars in Salmonella Ra outer core OS (Fig. 1A).…”

Section: Introductionmentioning

confidence: 99%

Molecular diversity of the genetic loci responsible for lipopolysaccharide core oligosaccharide assembly within the genus Salmonella

Kaniuk

Monteiro

Parker

et al. 2002

Molecular Microbiology

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SummaryThe waa locus on the chromosome of Salmonella enterica encodes enzymes involved in the assembly of the core oligosaccharide region of the lipopolysaccharide (LPS) molecule. To date, there are two known core structures in Salmonella , represented by serovars Typhimurium (subspecies I) and Arizonae (subspecies IIIA). The waa locus for serovar Typhimurium has been characterized. Here, the corresponding locus from serovar Arizonae is described, and the molecular basis for the distinctive structures is established. Eleven of the 13 open reading frames (ORFs) are shared by the two loci and encode conserved proteins of known function. Two polymorphic regions distinguish the waa loci. One involves the waaK gene, the product of which adds a terminal a a a a -1,2-linked Nacetylglucosamine residue that characterizes the serovar Typhimurium core oligosaccharide. There is an extensive internal deletion within waaK of serovar Arizonae. The serovar Arizonae locus contains a novel ORF ( waaH ) between the waaB and waaP genes. Structural analyses and in vitro glycosyltransferase assays identified WaaH as the UDPglucose:(glucosyl) LPS a a a a -1,2-glucosyltransferase responsible for the addition of the characteristic terminal glucose residue found in serovar Arizonae. Isolates comprising the Salmonella Reference Collections, SARC (representing the eight subspecies of S. enterica ) and SARB (representing subspecies I), were examined to assess the distribution of the waa locus polymorphic regions in natural populations. These comparative studies identified additional waa locus polymorphisms, shedding light on the genetic basis for diversity in the LPS core oligosaccharides of Salmonella isolates and identifying potential sources of further novel LPS structures.

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Screening for Salmonella with a Murine Monoclonal Antibody M105 Detects Both Felix O1 Bacteriophage Sensitive and Resistant Salmonella Strains

Cited by 6 publications

References 17 publications

The detection of Salmonella using a combined immunomagnetic separation and ELISA end-detection procedure

The detection of Salmonella using a combined immunomagnetic separation and ELISA end-detection procedure

Molecular insights into the assembly and diversity of the outer core oligosaccharide in lipopolysaccharides from Escherichia coli and Salmonella

Molecular diversity of the genetic loci responsible for lipopolysaccharide core oligosaccharide assembly within the genus Salmonella

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