Competitive adsorption of fibrinogen and dipalmitoylphosphatidylcholine at the air/aqueous interface

Restoration of the interfacial properties of lung surfactant with a newly designed hydrocarbon/fluorocarbon lipid

“…As previously described, the PEO groups create a steric barrier to the adsorption of proteins [59][60][61]. However, there are no results in the present literature similar to the those obtained with IPL [27,51,58]. Furthermore, similar results were obtained when IPL and fibrinogen were injected simultaneously to the PBS subphase, further supporting this model (Supplementary Data Figure S8).…”

“…Though this does not conclusively prove that IPL removed all fibrinogen molecules from the interface, it is clear that the monolayer behaved in a manner similar to that observed when significant amounts of fibrinogen were not present at the interface. Previous studies performed with DLPC or DPPC vesicles did not show this promising result of the prevention of protein adsorption in the presence fibrinogen pre-adsorbed to the monolayer [51,57,21,58].…”

“…It is known that fibrinogen inactivates the lung surfactant mixture [51]. The interfacial behavior and the stability of pure fibrinogen were also investigated, and both the -time and -A isotherms are presented at Supplementary Data (Figures S3 and S4).…”

“…Under stable conditions, fibrinogen molecules cannot adsorb to a previously adsorbed and stabilized DPPC monolayer [51]. Only if the DPPC monolayer was compressed and then expanded (i.e., if the monolayer is disturbed) does fibrinogen manage to adsorb to the interface and cause lipid desorption [30].…”

Effect of sonication and freezing–thawing on the aggregate size and dynamic surface tension of aqueous DPPC dispersions

“…These values remained essentially unchanged for 30 days or more, and the size distribution became slightly narrower with time. Hence, these DPPC dispersions produced by the protocol 2 contain vesicles and are quite stable [12,13].…”