Competence for regeneration of cucumber cotyledons is restricted to specific developmental stages

Colijn-Hooymans, C. M.; Hakkert, J.C.; Jansen, J.; Custers, J.B.M.

doi:10.1007/bf00035972

Cited by 54 publications

(34 citation statements)

References 20 publications

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“…This again stresses the importance of adopting optimized transformation techniques to minimize the time in culture and adopting strategies utilizing multigene constructs and simultaneous co-transformation rather than sequential transformation with different constructs or crosses between independently transformed lines (unless the transformed lines were backcrossed). These observations do not exclude the possibility of pre-existing variations in the explant (Colijn-Hooymans et al 1994). This is especially important in the case of the epigenetic program.…”

Section: The Effect Of Regeneration Technique -Somaclonal Variationmentioning

confidence: 94%

Unintended consequences of plant transformation: A molecular insight

Filipecki¹,

Malepszy²

2006

J Appl Genet

112

104

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Plant genomes are dynamic structures having both the system to maintain and accurately reproduce the information encoded therein and the ability to accept more or less random changes, which is one of the foundations of evolution. Crop improvement and various uncontrolled stress factors can induce unintended genetic and epigenetic variations. In this review it is attempted to summarize factors causing such changes and the molecular nature of these variations in transgenic plants. Unintended effects in transgenic plants can be divided into three main groups: first, pleiotropic effects of integrated DNA on the host plant genome; second, the influence of the integration site and transgene architecture on transgene expression level and stability; and third, the effect of various stresses related to tissue handling, regeneration and clonal propagation. Many of these factors are recently being redefined due to new researches, which apply modern highly sensitive analytical techniques and sequenced model organisms. The ability to inspect large portions of genomes clearly shows that tissue culture contributes to a vast majority of observed genetic and epigenetic changes. Nevertheless, monitoring of thousands transcripts, proteins and metabolites reveals that unintended variation most often falls in the range of natural differences between landraces or varieties. We expect that an increasing amount of evidence on many important crop species will support these observations in the nearest future.

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Section: The Effect Of Regeneration Technique -Somaclonal Variationmentioning

confidence: 94%

Unintended consequences of plant transformation: A molecular insight

Filipecki¹,

Malepszy²

2006

J Appl Genet

112

104

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“…Polysomaty occurs throughout plants of cucumber, Arabidopsis, tomato, cabbage and orchid, and ploidy increases with organ age (Galbraith et al, 1991;Gilissen et al, 1993;Smulders et al, 1994;Colijn-Hooymans et al, 1994;Kudo and Kimura, 2001;Yang and Loh, 2004). Changes in the ploidy of the explant due to maturity or aging (ColijnHooymans et al, 1994;Adelberg et al, 1994;Guis et al, 2000) are observed in regenerant populations.…”

Section: Production Of Chimeric Regenerantsmentioning

confidence: 95%

Adventitious regeneration in vitro occurs across a wide spectrum of squash (Cucurbita pepo) genotypes

Kathiravan

Vengedesan

Singer

et al. 2006

Plant Cell Tiss Organ Cult

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Cucurbita pepo L. (squash, pumpkin) is a highly polymorphic vegetable species of major importance. Our study characterized a spectrum of C. pepo germplasm for the ability to regenerate in vitro by direct organogenesis from cotyledon explants. Cultivars tested included both cultivated subspecies, texana and pepo, and nearly all of their respective cultivar-groups. Direct shoot regeneration occurred in all accessions, and was generally high (56-94%), with a single exception of 22% ('Bolognese'). There was no significant difference between the percentage regeneration of the two subspecies. Shoot regeneration per responding explant was uniform (1.2-1.6 shoots per explant). Only 'True French' produced statistically more shoots (3.9 per explant) than other accessions. The morphology of regeneration varied. Most cultivars produced long shoots, often fasciated, amid a few small buds. Some subspecies pepo cultivars (Beirut, Yugoslavia 7, Ma'yan and True French) produced short, massive, hollow shoots, sometimes accompanied by shoots that were more normal. Two subspecies texana cultivars (Creamy Straightneck and Small Bicolor) produced single (sometimes double) shoots without other buds. The production of chimeric (mixoploid) regenerants varied and there was a tendency to regenerate chimeric plants from the widest-fruited accessions (i.e. lowest length-to-width ratio) in each subspecies. Subspecies pepo Pumpkin Group 'Tondo di Nizza' showed significantly greater production of chimeric regenerants. In comparison with the great range of variation observed in fruit shape, the variation of in vitro responses (mostly less than 2-fold in regeneration and shoot production) was less than expected.

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“…This mutagenic stress-response mechanism can be described as a programmed loss of cellular control, leading to ploidy changes, chromosome rearrangements, DNA methylation, and point mutations (Phillips et al 1994). Additionally, plants regenerated in tissue culture can harbor changes induced in the cells during tissue differentiation in planta before explantation (Colijn-Hooymans et al 1994). Increase in ploidy level and changes in methylation pattern are somaclonal changes that are easily observed.…”

Section: Introductionmentioning

confidence: 99%

Tissue-culture-responsive and autotetraploidy-responsive changes in metabolic profiles of cucumber (Cucumis sativus L.)

Filipecki

Yin

Wiśniewska³

et al. 2006

J Appl Genet

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Somaclonal variation commonly occurs during in vitro plant regeneration and may introduce unintended changes in numerous plant characters. In order to assess the range of tissue-culture-responsive changes on the biochemical level, the metabolic profiles of diploid and tetraploid cucumber R1 plants regenerated from leaf-derived callus were determined. Gas chromatography and mass spectrometry were used for monitoring of 48 metabolites and many significant changes were found in metabolic profiles of these plants as compared to a seed-derived control. Most of the changes were common to diploids and tetraploids and were effects of tissue culture. However, tetraploids showed quantitative changes in 14 metabolites, as compared to regenerated diploids. These changes include increases in serine, glucose-6P, fructose-6P, oleic acid and shikimic acid levels. Basing on this study we conclude that the variation in metabolic profiles does not correlate directly with the range of genome changes in tetraploids.

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Competence for regeneration of cucumber cotyledons is restricted to specific developmental stages

Cited by 54 publications

References 20 publications

Unintended consequences of plant transformation: A molecular insight

Unintended consequences of plant transformation: A molecular insight

Adventitious regeneration in vitro occurs across a wide spectrum of squash (Cucurbita pepo) genotypes

Tissue-culture-responsive and autotetraploidy-responsive changes in metabolic profiles of cucumber (Cucumis sativus L.)

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