Tissue-culture-responsive and autotetraploidy-responsive changes in metabolic profiles of cucumber (Cucumis sativus L.)

Filipecki, Marcin; Yin, Zhimin; Wiśniewska, Anita; Śmiech, M.; Malinowski, Robert; Malepszy, S.

doi:10.1007/bf03194594

Cited by 9 publications

(4 citation statements)

References 14 publications

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“…Despite the presence of duplicated genes in tetraploids it may not cause duplicated transcription and translation and duplicated enzyme activity. Several works have shown variable effects of genome dosage on the production of metabolites . In accord with this information, cultivars of the same species do not contain the same amount of antioxidants, what was showed, for example, in twelve cultivars of wheat treated with low UV‐B dose .…”

Section: Discussionmentioning

confidence: 66%

Short‐term UV‐B Dose Stimulates Production of Protective Metabolites in Matricaria chamomilla Leaves

Petruľová

Dučaiová

Repčák

2014

Photochem & Photobiology

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Physiological response of two cultivars of Matricaria chamomilla plants on UV irradiation was studied. The impact of used short-time UV dose was evaluated in three time points; 2, 24 and 48 h after irradiation. Used UV irradiation immediately resulted in changes in plant oxidative status monitored as increased concentration of H2 O2 . Decrease in chlorophyll a and b indicated the impact on photosynthetic apparatus. For phenolic secondary metabolites, an increase in total soluble phenols and AlCl3 -reactive flavonols was observed. The activity of main phenolic enzyme, phenylalanine ammonia-lyase, increased with time after irradiation. Significant changes, mainly decreasing trends, in the content of free coumarins and their glycosidic precursors were observed. Enhanced accumulation in chlorogenic and 1,5-dicaffeoylquinic acid and in (Z)-isoform of dicycloethers was detected. From these results, the redirecting precursors of coumarin biosynthesis to biosynthesis of substances with higher antioxidative potential can be assumed. Different reactions in diploid and tetraploid plants were recorded, too.

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Section: Discussionmentioning

confidence: 66%

Short‐term UV‐B Dose Stimulates Production of Protective Metabolites in Matricaria chamomilla Leaves

Petruľová

Dučaiová

Repčák

2014

Photochem & Photobiology

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“…The tetraploid Lycopersicum esculentum showed a diverse enzymatic response to polyploidization, i.e., malate dehydrogenase, acid invertase and acid phosphatase activity increased, while peroxidase activity decreased and neutral phosphatase and esterase concentrations were not affected (reviewed in [ 53 ]). In a metabolic comparison between tetraploid lines generated from the diploid Cucumis sativus L., Filipecki et al [ 56 ] concluded that differences in metabolic profiles were tissue-culture responsive and did not correlate directly with the range of genome changes in tetraploids. We note here that while all of the above studies have made a valuable contribution to understanding the effects of gene dosage, these studies were targeted to specific metabolites.…”

Section: Discussionmentioning

confidence: 99%

Morphological, cytological and metabolic consequences of autopolyploidization in Hylocereus (Cactaceae) species

2013

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BackgroundGenome doubling may have multi-level effects on the morphology, viability and physiology of polyploids compared to diploids. We studied the changes associated with autopolyploidization in two systems of somatic newly induced polyploids, diploid-autotetraploid and triploid-autohexaploid, belonging to the genus Hylocereus (Cactaceae). Stomata, fruits, seeds, embryos, and pollen were studied. Fruit pulp and seeds were subjected to metabolite profiling using established gas chromatography-mass spectrometry (GC-MS) and ultra-performance liquid chromatography (UPLC) Q-TOF-MS/MS (time of flight)-protocols.ResultsAutopolyploid lines produced lower numbers of tetrads, larger pollen grains with lower viability, larger stomata with lower density, and smaller fruits with lower seed numbers and decreased seed viability. The abundance of sugars was lower in the fruits and seeds of the two duplicated lines than in their donor lines, accompanied by increased contents of amino acids, tricarboxylic acid (TCA) cycle intermediates, organic acids and flavonoids. Betacyanins, the major fruit pigments in diploid and triploid donors, decreased following genome doubling. Both autopolyploid Hylocereus lines thus exhibited unfavorable changes, with the outcome being more dramatic in the autohexaploid than in the autotetraploid line.ConclusionInduced autotetraploid and autohexaploid lines exhibited morphological and cytological characteristics that differed from those of their donor plants and that were accompanied by significant metabolic alterations. It is suggested that a developmental arrest occurs in the fruits of the autohexaploid line, since their pericarp shows a greater abundance of acids and of reduced sugars. We conclude that genome doubling does not necessarily confer a fitness advantage and that the extent of alterations induced by autopolyploidization depends on the genetic background of the donor genotype.

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“…cepa [9], A . fistulosum [10], Solanum tuberosum [11], Cucumis sativus L. [12] and Agapanthus praecox [13] but also in the model dicotyledonous plant Arabidopsis thaliana [14]. Considerable changes in the global DNA methylation levels as well as methylation at specific sites have been demonstrated for tissue cultures of oil palm [15], grapevine [16] and apple [17].…”

Section: Introductionmentioning

confidence: 99%

Nuclear genome stability in long-term cultivated callus lines of Fagopyrum tataricum (L.) Gaertn

et al. 2017

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Long-term cultivated Fagopyrum tataricum (L.) Gaertn. (Tartary buckwheat) morphogenic and non-morphogenic callus lines are interesting systems for gaining a better understanding of the mechanisms that are responsible for the genetic stability and instability of a plant tissue culture. In this work, we used histological sections and transmission electron microscopy to identify and describe the morphology of the nuclei of all of the analysed callus lines. We demonstrated that the embryogenic callus cells had prominent round nuclei that did not contain heterochromatin clumps in contrast to the non-morphogenic callus lines, in which we found nuclei that had multiple lobes. Flow cytometry analysis revealed significant differences in the relative DNA content between the analysed calli. All of the analysed morphogenic callus lines had peaks from 2C to 8C as compared to the non-morphogenic callus lines, whose peaks did not reflect any regular DNA content and exceeded 8C and 16C for the line 6p1 and 16C and 32C for the callus line 10p2A. The results showed that non-morphogenic calli are of an aneuploid nature. The TUNEL test enabled us to visualise the nuclei that had DNA fragmentation in both the morphogenic and non-morphogenic lines. We revealed significantly higher frequencies of positively labelled nuclei in the non-morphogenic lines than in the morphogenic lines. In the case of the morphogenic lines, the highest observed frequency of TUNEL-positive nuclei was 7.7% for lines 2–3. In the non-morphogenic calli, the highest level of DNA damage (68.5%) was revealed in line 6p1. These results clearly indicate greater genome stability in the morphogenic lines.

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Tissue-culture-responsive and autotetraploidy-responsive changes in metabolic profiles of cucumber (Cucumis sativus L.)

Cited by 9 publications

References 14 publications

Short‐term UV‐B Dose Stimulates Production of Protective Metabolites in Matricaria chamomilla Leaves

Short‐term UV‐B Dose Stimulates Production of Protective Metabolites in Matricaria chamomilla Leaves

Morphological, cytological and metabolic consequences of autopolyploidization in Hylocereus (Cactaceae) species

Nuclear genome stability in long-term cultivated callus lines of Fagopyrum tataricum (L.) Gaertn

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