Compatible solutes adaptive alterations in Arthrobacter simplex during exposure to ethanol, and the effect of trehalose on the stress resistance and biotransformation performance

Cheng, Hong-Jin; Sun, Ya-hua; Chang, Huang-Kuang; Cui, Fangfang; Xue, Haijie; Shen, Yanbing; Wang, Min; Luo, Jianmei

doi:10.1007/s00449-020-02286-9

Cited by 12 publications

(6 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Good tolerance toward ethanol is critical for the industrial application of enzymes because adding ethanol into the reaction system is helpful for the dissolution of nonpolar substrates ( Ogino, 2009 ; Cheng et al, 2020 ). Therefore, we investigated the effect of ethanol on enzyme activity, and the results were presented as heatmaps ( Figure 5 ).…”

Section: Resultsmentioning

confidence: 99%

Immobilized Crosslinked Pectinase Preparation on Porous ZSM-5 Zeolites as Reusable Biocatalysts for Ultra-Efficient Hydrolysis of β-Glycosidic Bonds

et al. 2021

View full text Add to dashboard Cite

In this study, we immobilized pectinase preparation on porous zeolite ZSM-5 as an enzyme carrier. We realized this immobilized enzyme catalyst, pectinase preparation@ZSM-5, via a simple combined strategy involving the van der Waals adsorption of pectinase preparation followed by crosslinking of the adsorbed pectinase preparation with glutaraldehyde over ZSM-5. Conformal pectinase preparation coverage of various ZSM-5 supports was achieved for the as-prepared pectinase preparation@ZSM-5. The porous pectinase preparation@ZSM-5 catalyst exhibited ultra-efficient biocatalytic activity for hydrolyzing the β-glycosidic bonds in the model substrate 4-nitrophenyl β-D-glucopyranoside, with a broad operating temperature range, high thermal stability, and excellent reusability. The relative activity of pectinase preparation@ZSM-5 at a high temperature (70 °C) was nine times higher than that of free pectinase preparation. Using thermal inactivation kinetic analysis based on the Arrhenius law, pectinase preparation@ZSM-5 showed higher activation energy for denaturation (315 kJ mol−1) and a longer half-life (62 min−1) than free pectinase preparation. Moreover, a Michaelis–Menten enzyme kinetic analysis indicated a higher maximal reaction velocity for pectinase preparation@ZSM-5 (0.22 µmol mg−1 min−1). This enhanced reactivity was attributed to the microstructure of the immobilized pectinase preparation@ZSM-5, which offered a heterogeneous reaction system that decreased the substrate–pectinase preparation binding affinity and modulated the kinetic characteristics of the enzyme. Additionally, pectinase preparation@ZSM-5 showed the best ethanol tolerance among all the reported pectinase preparation-immobilized catalysts, and an activity 247% higher than that of free pectinase preparation at a 10% (v/v) ethanol concentration was measured. Furthermore, pectinase preparation@ZSM-5 exhibited potential for practical engineering applications, promoting the hydrolysis of β-glycosidic bonds in baicalin to convert it into baicalein. This was achieved with a 98% conversion rate, i.e., 320% higher than that of the free enzyme.

show abstract

Section: Resultsmentioning

confidence: 99%

Immobilized Crosslinked Pectinase Preparation on Porous ZSM-5 Zeolites as Reusable Biocatalysts for Ultra-Efficient Hydrolysis of β-Glycosidic Bonds

et al. 2021

View full text Add to dashboard Cite

show abstract

“…To compensate for high osmotic pressure, microorganisms often maintain intracellular water by synthesizing large amounts of endogenous osmo-protectants such as trehalose. Overexpression of the trehalose biosynthetic genes (otsA, otsB, and treS) from Arthrobacter simplex in E. coli can induce the cell to synthesize trehalose as an osmo-protector, thereby greatly improving its tolerance to ethanol (Cheng et al 2020).…”

Section: Stress Protein Engineeringmentioning

confidence: 99%

Strategies to increase the robustness of microbial cell factories

Xu,

Lin,

Zhang

et al. 2024

Adv. Biotechnol.

View full text Add to dashboard Cite

Engineering microbial cell factories have achieved much progress in producing fuels, natural products and bulk chemicals. However, in industrial fermentation, microbial cells often face various predictable and stochastic disturbances resulting from intermediate metabolites or end product toxicity, metabolic burden and harsh environment. These perturbances can potentially decrease productivity and titer. Therefore, strain robustness is essential to ensure reliable and sustainable production efficiency. In this review, the current strategies to improve host robustness were summarized, including knowledge-based engineering approaches, such as transcription factors, membrane/transporters and stress proteins, and the traditional adaptive laboratory evolution based on natural selection. Computation-assisted (e.g. GEMs, deep learning and machine learning) design of robust industrial hosts was also introduced. Furthermore, the challenges and future perspectives on engineering microbial host robustness are proposed to promote the development of green, efficient and sustainable biomanufacturers.

show abstract

“…26 Trehalose biosynthesis genes (otsA, otsB, and treS) from Arthrobacter simplex can also be overexpressed in E. coli to produce osmoprotectant trehalose, which can serve as a good protectant to maintain the cell integrity and membrane stability, thus greatly improving the survival rate after shock by 10% (vol/vol) ethanol. 27…”

Section: Biosynthesis Of Protective Agents Against Osmotic Pressurementioning

confidence: 99%

“…To solve this problem, the otsBA operon responsible for the trehalose biosynthesis can be overexpressed to help tolerate the toxic impurities in crude glycerol, which increased the β ‐carotene production to 150.9 mg L −1 from 40 g L −1 crude glycerol 26 . Trehalose biosynthesis genes ( otsA , otsB , and treS ) from Arthrobacter simplex can also be overexpressed in E. coli to produce osmoprotectant trehalose, which can serve as a good protectant to maintain the cell integrity and membrane stability, thus greatly improving the survival rate after shock by 10% (vol/vol) ethanol 27 …”

Section: Regulation Factors Engineeringmentioning

confidence: 99%

Strategies to improve the stress resistance of Escherichia coli in industrial biotechnology

Tao

Wang

et al. 2022

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

The use of industrial Escherichia coli strains for the production of bio‐based chemicals offers a promising and sustainable solution to a growing scarcity of non‐renewable resources and contributes to the protection of the environment. However, many limitations, such as inhibition from toxic substrates, hyperosmosis, toxic by‐products, and other adverse fermentation conditions, prevent strains from attaining a stable cell growth rate, efficient metabolic flux, and satisfactory productivity. There has thus been much effort to obtain E. coli strains that exhibit robust growth and productivity with enhanced tolerance of extreme operating conditions. In this review, we gained insights into recent studies in improving tolerance of E. coli and summarized four kinds of engineering methods: modifying regulation factors, regulating membrane structure, optimizing biosynthetic pathways, and applying adaptive evolution. The combination of different engineering strategies therefore provided a broader platform for robustness of industrial E. coli, making further steps to achieving more advantageous biosynthesis. © 2022 Society of Chemical Industry and John Wiley & Sons, Ltd

show abstract

Compatible solutes adaptive alterations in Arthrobacter simplex during exposure to ethanol, and the effect of trehalose on the stress resistance and biotransformation performance

Cited by 12 publications

References 46 publications

Immobilized Crosslinked Pectinase Preparation on Porous ZSM-5 Zeolites as Reusable Biocatalysts for Ultra-Efficient Hydrolysis of β-Glycosidic Bonds

Immobilized Crosslinked Pectinase Preparation on Porous ZSM-5 Zeolites as Reusable Biocatalysts for Ultra-Efficient Hydrolysis of β-Glycosidic Bonds

Strategies to increase the robustness of microbial cell factories

Strategies to improve the stress resistance of Escherichia coli in industrial biotechnology

Contact Info

Product

Resources

About