Compartmentalization of a synergistic fungal-bacterial consortium to boost lactic acid conversion from lignocellulose <i>via</i> consolidated bioprocessing

Jiang, Yujia; Yang, Xinyi; Pan, Runze; Mou, Lu; Jiang, Wankui; Zhang, Wenming; Xin, Fengxue; Jiang, Min

doi:10.1039/d2gc04520f

Cited by 13 publications

(3 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although compartmentalization approaches have been developed to e.g. simultaneously provide the required oxygen profiles for cellulose-degrading and bioproduct-forming organisms in consolidated processes [ 25 , 26 ], such approaches may not be transferable to other abiotic factors, such as temperature, or production-relevant reactor systems, such as stirred-tank bioreactors (STRs).…”

Section: Introductionmentioning

confidence: 99%

Synthetic co-culture in an interconnected two-compartment bioreactor system: violacein production with recombinant E. coli strains

Müller,

Schick,

Klemp

et al. 2024

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

The concept of modular synthetic co-cultures holds considerable potential for biomanufacturing, primarily to reduce the metabolic burden of individual strains by sharing tasks among consortium members. However, current consortia often show unilateral relationships solely, without stabilizing feedback control mechanisms, and are grown in a shared cultivation setting. Such ‘one pot’ approaches hardly install optimum growth and production conditions for the individual partners. Hence, novel mutualistic, self-coordinating consortia are needed that are cultured under optimal growth and production conditions for each member. The heterologous production of the antibiotic violacein (VIO) in the mutually interacting E. coli–E. coli consortium serves as an example of this new principle. Interdependencies for growth control were implemented via auxotrophies for L-tryptophan and anthranilate (ANT) that were satisfied by the respective partner. Furthermore, VIO production was installed in the ANT auxotrophic strain. VIO production, however, requires low temperatures of 20–30 °C which conflicts with the optimum growth temperature of E. coli at 37 °C. Consequently, a two-compartment, two-temperature level setup was used, retaining the mutual interaction of the cells via the filter membrane-based exchange of medium. This configuration also provided the flexibility to perform individualized batch and fed-batch strategies for each co-culture member. We achieved maximum biomass-specific productivities of around 6 mg (g h)−1 at 25 °C which holds great promise for future applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Synthetic co-culture in an interconnected two-compartment bioreactor system: violacein production with recombinant E. coli strains

Müller,

Schick,

Klemp

et al. 2024

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

show abstract

“…CBP process is usually based on synergistic microbial consortia [ 100 , 101 ]. In this case, lignocellulose degradation microorganism is used to overproduce fermented sugar via secreted cellulolytic degrading enzyme, which can be further converted to produce other biochemical using engineered microbial cell factories.…”

Section: Introductionmentioning

confidence: 99%

“…For instance, 19.8 g/L LA was obtained via a synergistic fungal–bacterial ( Trichoderma reesei/Lactobacillus pentosus ) consortium system using non-detoxified steam pretreatment of beech wood as a feedstock [ 100 ]. In another study, Jiang, et al developed a new synergistic fungal–bacterial ( Trichoderma asperellum/Lactobacillus paracasei ) consortium system, which can directly produce 14.9 g/L LA from corncob as a feedstock without any prior pretreatment process [ 101 ]. Thus, constructing tolerant LA strains combined with these novel synergistic microbial consortia will further improve the economic benefit of cellulosic LA production in future.…”

Section: Introductionmentioning

confidence: 99%

Microbial tolerance engineering for boosting lactic acid production from lignocellulose

Shan

Yan

et al. 2023

Biotechnol Biofuels

View full text Add to dashboard Cite

Lignocellulosic biomass is an attractive non-food feedstock for lactic acid production via microbial conversion due to its abundance and low-price, which can alleviate the conflict with food supplies. However, a variety of inhibitors derived from the biomass pretreatment processes repress microbial growth, decrease feedstock conversion efficiency and increase lactic acid production costs. Microbial tolerance engineering strategies accelerate the conversion of carbohydrates by improving microbial tolerance to toxic inhibitors using pretreated lignocellulose hydrolysate as a feedstock. This review presents the recent significant progress in microbial tolerance engineering to develop robust microbial cell factories with inhibitor tolerance and their application for cellulosic lactic acid production. Moreover, microbial tolerance engineering crosslinking other efficient breeding tools and novel approaches are also deeply discussed, aiming to providing a practical guide for economically viable production of cellulosic lactic acid.

show abstract

Modularization of Immobilized Multienzyme Cascades for Continuous‐Flow Enantioselective C−H Amination

Kong,

Huang,

Zhou

et al. 2024

Angewandte Chemie

View full text Add to dashboard Cite

Multienzyme cascades (MECs) have gained much attention in synthetic chemistry but remain far from being a reliable synthetic tool. Here we report a four‐enzyme cascade comprising a cofactor‐independent and a cofactor self‐sustaining bienzymatic module for the enantioselective benzylic C‐H amination of arylalkanes, a challenging transformation from bulk chemicals to high value‐added chiral amines. The two modules were subsequently optimized by enzyme co‐immobilization with microenvironmental tuning, and finally integrated in a gas‐liquid segmented flow system, resulting in simultaneous improvements in enzyme performance, mass transfer, system compatibility, and productivity. The flow system enabled continuous C‐H amination of arylalkanes (up to 100 mM) utilizing the sole cofactor NADH (0.5 mM) in >90% conversion, achieving a high space‐time yield (STY) of 3.6 g·L ‐1 ·h ‐1 , which is a 90‐fold increase over previously reported values.

show abstract

Compartmentalization of a synergistic fungal-bacterial consortium to boost lactic acid conversion from lignocellulose via consolidated bioprocessing

Abstract: Consolidated bioprocessing (CBP) is a promising approach to directly convert lignocellulose feedstocks into targeted biochemicals and biofuels, which is commonly executed by single engineered microorganisms. Alternatively, the complexed metabolic functions...

Cited by 13 publications

References 32 publications

Synthetic co-culture in an interconnected two-compartment bioreactor system: violacein production with recombinant E. coli strains

Synthetic co-culture in an interconnected two-compartment bioreactor system: violacein production with recombinant E. coli strains

Microbial tolerance engineering for boosting lactic acid production from lignocellulose

Modularization of Immobilized Multienzyme Cascades for Continuous‐Flow Enantioselective C−H Amination

Contact Info

Product

Resources

About