Comparisons of Two By-Products and a Prepared Diet as Organic Fertilizers on Growth and Survival of Larval Paddlefish,<i>Polyodon spathula,</i>in Earthen Ponds

Mims, Steven D.; Clark, Julia A.; Williams, J. F.; Rouse, David B.

doi:10.1300/j028v02n03_09

Cited by 16 publications

(13 citation statements)

References 8 publications

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“…The paddlefish Polyodon spathula Walbaum, 1792 is an important species in the United States (Carlson and Bonislawsky, 1981;Mims et al, 1993). The basic reproductive methods to reproduce paddlefish have been developed by Needham (1965) and Graham et al (1986).…”

Section: Introductionmentioning

confidence: 99%

Effect of cryoprotectants and male on motility parameters and fertilization rate in paddlefish (Polyodon spathula) frozen-thawed spermatozoa

Linhart¹,

Mims²,

Gomelsky³

et al. 2006

J Appl Ichthyol

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A sperm cryopreservation method using different cryoprotectants and sperm from different males was developed. Different percentages of pure cryoprotectants (methanol and DMSO) were added to extender 1 or 2 (20 mM tris pH 8 + 30 mM sucrose + 0.5 mM KCl or 20 mM tris pH 8 + 50 mM sucrose + 0.5 mM KCl, dilution 1:1) or non-extended sperm and every 1 ml of mixture was transferred to a 2-ml cryotube. The cryotubes were directly transferred to a pre-programmed PLANER Kryo 10 series III freezer at 0 o C and cooled from 0 o C to -5 o C at a rate of 3 o C.min -1 , from -5 o C to -15 o C at a rate of 5 o C.min -1 , from -15 o C to -25 o C at a rate of 10 o C .min -1 , from -25 o C to -80 o C at a rate of 20 o C .min -1 . Thereafter the samples were held for 5 min at -80 o C and finally transferred into LN 2 until next morning. The sperm was then thawed in a water bath at 40 o C for 105 s. Fertilization rate of control sperm was 81.5% (kept unfrozen; samples tested after 24-h storage at 3 o C), indicating that the gametes were of good quality. Percentage and the velocity of motile sperm from were evaluated in fresh and post-thawed sperm using video frames and subsequent image analysis. The results on hatching rates were significantly correlated with post-thawed sperm motility (r=0.49, P=0.035) and velocity (r=0.55, P=0.014) and not correlated with velocity of post-thawed spermatozoa (r=0.32, P=0.177). The best fertilization rates were obtained with 64-75 % in post-thawed sperm (3.6.10 5 spermatozoa per egg) when sperm were treated either without any extender or with both extenders with methanol concentrations of 8 or 10 %. These results were not significantly different compared with those obtained using fresh sperm control samples. Hatching rate was very low, only 8-15 %, when sperm was frozen with 8 or 10 % DMSO. ANOVA showed a significant effect of males on sperm motility, velocity and fertilization rate in post-thawed sperm.

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Section: Introductionmentioning

confidence: 99%

Effect of cryoprotectants and male on motility parameters and fertilization rate in paddlefish (Polyodon spathula) frozen-thawed spermatozoa

Linhart¹,

Mims²,

Gomelsky³

et al. 2006

J Appl Ichthyol

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“…The paddlefish Polyodon spathula Walbaum, 1792 and shovelnose sturgeon Scuphirhynchus platorynchus Rafinesque, 1820 are important species in the United States (Carlson & Bonislawsky, 1981;Mims et al, 1993). The basic methods for artificial reproduction have been developed for paddlefish by Needham (1965) and Graham et al (1986), and for shovelnose sturgeon by Conte et al (1988) in the United States.…”

Section: Introductionmentioning

confidence: 99%

Motility of spermatozoa from shovelnose sturgeon and paddlefish

Linhart

Mims

Shelton

1995

Journal of Fish Biology

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The spermatozoa in the seminal plasma from shovelnose sturgeon Scuphirhynchi6s pluforynchus and paddlefish Polyodon sputhulu were immotile with only a few spontaneously motile spermatozoa for 5-10 and 10-20 s, respectively. Spermatozoa of shovelnose sturgeon were observed to be 100% motile immediately after sperm dilution in 10mM NaCl and 20mM Tris-HCI, pH 8.5. The duration of mass progressive movement was 2-3 min; and 1 to 5% of spermatozoa remain active after 360 s (P

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“…Cottonseed meal with low carbon and high N and P (Geiger 1983;Anderson 1993b) has promoted high pH (Ͼ10) in striped bass fingerling production ponds, resulting in low fish survival (Anderson 1993a). Rice bran, which has a high carbon and low N and P content (Anderson 1993b), achieved moderate pH (range ϭ 7.4-8.6) and better water quality and zooplankton production than cottonseed meal or distiller's grain in ponds used to produce paddlefish Polyodon spathula (Mims et al 1993). Ludwig and Tackett (1991), however, found no significant difference in pH in ponds fertilized with rice bran or cottonseed meal.…”

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confidence: 99%

“…The pH of these cultures varied from 7.6 to 8.2, which overlapped the optimal pH range (6.8-7.8) for the culture of daphnia (Ivleva 1973). It appears that organic fertilizers that directly serve as a food source for zooplankton could better enhance secondary productivity and ultimately fish production when they also promote moderate pH (Mims et al 1993).…”

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confidence: 99%

Effects of Rice Bran, Cottonseed Meal, and Alfalfa Meal on pH and Zooplankton

et al. 2005

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Rice bran (RB), cottonseed meal (CSM), and alfalfa meal (ALF) were compared as organic fertilizers for their effects on pH and zooplankton abundance in limnocorrals that lacked fish. The weight (W) and nitrogen (N) content of CSM, which served as the control, were used to determine the other applications (RB‐N, RB‐W, ALF‐N and ALF‐W). Temperature, dissolved oxygen, chlorophyll a, ammonia nitrogen, and zooplankton densities were monitored for 8 weeks to simulate the average period for phase 1 production of striped bass Morone saxatilis (38–50 mm). Rice bran promoted better water quality than ALF or CSM when application rates were based on nitrogen content. The pH was lowest for RB‐N, intermediate for ALF‐N, and highest for CSM. Chlorophyll a was a function of nitrogen input, and the high‐nitrogen applications (CSM, ALF‐N, and RB‐N) supported similar phytoplankton standing crops that were 38–62% greater than the corresponding low‐nitrogen applications (ALF‐W and RB‐W). Water temperature and dissolved oxygen concentrations were similar among applications, and total ammonia nitrogen was below detectable levels for all applications. Overall mean densities of rotifers, nauplii, adult copepods, adult crustaceans, and total zooplankton did not differ significantly among applications. However, the mean density of cladocerans was significantly higher for RB‐N and ALF‐N than for ALF‐W. The densities of cladocerans, adult copepods, adult crustaceans, and total zooplankton correlated strongly with chlorophyll a but not with the quantities of fertilizers applied, indicating that phytoplankton probably was the primary food for these zooplankters. Based on pH, RB was better than CSM or ALF, but all had similar effects on zooplankton when application rates were based on nitrogen.

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Comparisons of Two By-Products and a Prepared Diet as Organic Fertilizers on Growth and Survival of Larval Paddlefish,Polyodon spathula,in Earthen Ponds

Cited by 16 publications

References 8 publications

Effect of cryoprotectants and male on motility parameters and fertilization rate in paddlefish (Polyodon spathula) frozen-thawed spermatozoa

Effect of cryoprotectants and male on motility parameters and fertilization rate in paddlefish (Polyodon spathula) frozen-thawed spermatozoa

Motility of spermatozoa from shovelnose sturgeon and paddlefish

Effects of Rice Bran, Cottonseed Meal, and Alfalfa Meal on pH and Zooplankton

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