Comparisons of the Structural Proteins of Avian Infectious Bronchitis Virus as Determined by Western Blot Analysis

Sneed, Loyd; Butcher, Gary D.; Parr, Rebecca D.; Wang, Li; Collisson, Ellen W.

doi:10.1089/vim.1989.2.221

Cited by 27 publications

(25 citation statements)

References 14 publications

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“…Although antigenic epitopes on IBV proteins are not well defined, it is believed that a number of conserved epitopes which give rise to cross-reactive antibodies exist on the SI, S2, N and M proteins (Ignjatovic & Galli, 1995). Strains isolated in the USA were shown to be antigenically similar with extensive cross-reaction in WB between heterologous sera and the S, N, and M proteins of nine strains (Sneed et al, 1989). In the same study the Australian Nl/62 ('T') strain, which belongs to the group I of classical IBV strains, was also antigenically similar to the USA strains.…”

Section: Discussionmentioning

confidence: 99%

A long‐term study of Australian infectious bronchitis viruses indicates a major antigenic change in recently isolated strains

1997

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The antigenic relationship among 36 IBV strains isolated between 1961 and 1994 from vaccinated and non-vaccinated chicken flocks was determined. Based on the reaction with nine monoclonal antibodies (MAbs) in ELISA and polyclonal chicken sera in western blotting, IBV strains clearly fell into two distinct antigenic groups. Nineteen IBV strains isolated between 1961 and 1994 from various locations were antigenically related, having common cross-reactive epitopes on the peplomer S, the nucleocapsid N and the membrane M proteins. IBV strains within this classical group could be antigenically differentiated further by serotyping and by their reaction with MAbs. Seventeen IBV strains isolated between 1988 and 1994, shared only a minor degree of antigenic similarity with strains in the classical group. Strains in this novel group were antigenically related to each other and shared cross-reactive epitopes particularly on the N and M proteins. The novel IBV strains were not detected before 1988 and their origin is unknown. They appeared suddenly and almost simultaneously at two distant commercial sites, Redland Bay and Appin, and were also isolated at a third location in Victoria 3 years later. The Appin strains persisted on the site for 3 years without changes in antigenicity, including the serotype; however, following introduction of vaccination with novel strains a variant of new serotype was isolated. Variants isolated in Victoria on the other hand showed greater antigenic diversity and tendency for change. Novel strains have not displaced classical strains which continued to be isolated frequently.

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Section: Discussionmentioning

confidence: 99%

A long‐term study of Australian infectious bronchitis viruses indicates a major antigenic change in recently isolated strains

1997

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“…Immune chickens were generated by inoculating 1-week-old chickens with 10 7 EID 50 of the IBV Gray strain by the eye-nasal routes. The virus, propagated by inoculating the allantoic sac of 11-day-old chicken embryos with the Gray strain of IBV and harvesting allantoic fluid 36 h p.i., was used for in vivo inoculation [34]. The IBV antigen used in the ELISA and ELISPOT assay was purified by polyethylene glycol (PEG) 8000 precipitation.…”

Section: Animals and Virusmentioning

confidence: 99%

Specific antibody secreting cells from chickens can be detected by three days and memory B cells by three weeks post-infection with the avian respiratory coronavirus

Pei

Collisson

2005

Developmental & Comparative Immunology

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Infectious bronchitis virus (IBV), the first coronavirus described, has been a continuing problem in poultry for more than 70 years. IBV, causing a highly contagious respiratory disease in chickens, resembles the recently described severe acute respiratory syndrome virus in pathogenesis and genome organization. While previous studies demonstrated that effector and memory CD8(+) T lymphocytes are critical in controlling acute IBV infection and disease in chickens, here chicken anti-IBV antibody (IgG) secreting cells (ASC) in both peripheral blood mononuclear cells (PBMC) and spleens collected following IBV Gray infection were evaluated using an ELISPOT assay. The ASC in peripheral blood and spleens can be detected from 3 to 7 days post-infection (p.i.), which is 3-7 days earlier than anti-IBV IgG detected in the serum. The ASC frequency reached a maximum at 7-10 days p.i., and decreased more than 90% in the spleen and 70% in PBMC by 14 days p.i. The ASC levels in the PBMC then decreased gradually to 0.5 ASC/10(6) over the next 8 weeks. The higher concentration of about 20 ASC/10(6) cells in spleens may, at least partially, account for the presence of antibody in the serum although bone marrow ASC were not determined. In vitro stimulation of PBMC and splenocytes with IBV antigen demonstrated that memory B cells can be activated to secrete antibody by 3 weeks p.i. ELISPOT detection of primary B cells could be useful in the early detection of infection following infection with respiratory coronaviruses.

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“…The pneumopathogenic M41 and the moderately pneumopathogenic and moderately nephropathogenic Gray strains of IBV were separately propagated in 10-day-old specific pathogen free (SPF) chicken embryos (Sneed et al, 1989). The morbidity (any respiratory illness during any of the days examined) resulting from infection with either strain was >90% in these studies.…”

Section: Viral Stocksmentioning

confidence: 99%

Association of the chicken MHC B haplotypes with resistance to avian coronavirus

Banat

Tkalčić

Dzielawa

et al. 2013

Developmental & Comparative Immunology

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Clinical respiratory illness was compared in five homozygous chicken lines, originating from homozygous B2, B8, B12 and B19, and heterozygous B2/B12 birds after infection with either of two strains of the infectious bronchitis virus (IBV). All chickens used in these studies originated from White Leghorn and Ancona linages. IBV Gray strain infection of MHC homozygous B12 and B19 haplotype chicks resulted in severe respiratory disease compared to chicks with B2/B2 and B5/B5 haplotypes. Demonstrating a dominant B2 phenotype, B2/B12 birds were also more resistant to IBV. Respiratory clinical illness in B8/B8 chicks was severe early after infection, while illness resolved similar to the B5 and B2 homozygous birds. Following M41 strain infection, birds with B2/B2 and B8/B8 haplotypes were again more resistant to clinical illness than B19/B19 birds. Real time RT-PCR indicated that infection was cleared more efficiently in trachea, lungs and kidneys of B2/B2 and B8/B8 birds compared with B19/B19 birds. Furthermore, M41 infected B2/B2 and B8/B8 chicks performed better in terms of body weight gain than B19/B19 chicks. These studies suggest that genetics of B defined haplotypes might be exploited to produce chicks resistant to respiratory pathogens or with more effective immune responses.

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Comparisons of the Structural Proteins of Avian Infectious Bronchitis Virus as Determined by Western Blot Analysis

Cited by 27 publications

References 14 publications

A long‐term study of Australian infectious bronchitis viruses indicates a major antigenic change in recently isolated strains

A long‐term study of Australian infectious bronchitis viruses indicates a major antigenic change in recently isolated strains

Specific antibody secreting cells from chickens can be detected by three days and memory B cells by three weeks post-infection with the avian respiratory coronavirus

Association of the chicken MHC B haplotypes with resistance to avian coronavirus

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