Association of the chicken MHC B haplotypes with resistance to avian coronavirus

Abstract

Clinical respiratory illness was compared in five homozygous chicken lines, originating from homozygous B2, B8, B12 and B19, and heterozygous B2/B12 birds after infection with either of two strains of the infectious bronchitis virus (IBV). All chickens used in these studies originated from White Leghorn and Ancona linages. IBV Gray strain infection of MHC homozygous B12 and B19 haplotype chicks resulted in severe respiratory disease compared to chicks with B2/B2 and B5/B5 haplotypes. Demonstrating a dominant B2 phenotype, B2/B12 birds were also more resistant to IBV. Respiratory clinical illness in B8/B8 chicks was severe early after infection, while illness resolved similar to the B5 and B2 homozygous birds. Following M41 strain infection, birds with B2/B2 and B8/B8 haplotypes were again more resistant to clinical illness than B19/B19 birds. Real time RT-PCR indicated that infection was cleared more efficiently in trachea, lungs and kidneys of B2/B2 and B8/B8 birds compared with B19/B19 birds. Furthermore, M41 infected B2/B2 and B8/B8 chicks performed better in terms of body weight gain than B19/B19 chicks. These studies suggest that genetics of B defined haplotypes might be exploited to produce chicks resistant to respiratory pathogens or with more effective immune responses.

Establishment of six homozygous MHC-B haplotype populations associated with susceptibility to Marek’s disease in Chinese specific pathogen-free BWEL chickens

“…It is compact, simple, considerably smaller than its mammalian counterpart, and strongly associated with resistance and susceptibility to certain avian diseases, including Marek's disease (MD) (Bacon et al, 2001), Rous sarcoma (Taylor, 2004), lymphoid leukosis (Bacon et al, 1981), infectious bronchitis (Banat et al, 2013), coccidiosis (Miller et al, 1994), and salmonellosis (Cotter et al, 1998). The chicken MHC comprises two regions, the MHC-B and the MHC-Y regions, both of which map to chromosome 16 (Miller et al, 2004).…”

Major Histocompatibility Complex-Associated Resistance to Infectious Diseases: The Case of Bovine Leukemia Virus Infection

“…Based on data registered in the Domestic Animal Diversity Information System (DAD-IS) 2015 report [42], 483 mammalian breed populations are recorded as having resistance or tolerance to Not unexpectedly, the high degree of genetic polymorphism in the MHC has been associated with health status, vaccine responsiveness, and production traits in cattle [43][44][45][46][47]. Examples of some studies for which there is documented evidence of MHC association with resistance or susceptibility to disease include mastitis in cattle and sheep [44,48], tick-borne disease [49][50][51], dermatophilosis in cattle [52], enzootic bovine leucosis in cattle and sheep [53][54][55][56], neosporosis in cattle [57], theileriosis in cattle [58], gastrointestinal parasites in sheep [59,60], diarrhea in pigs [61,62], Marek's disease in chicken [63,64], coccidiosis in chicken [65], and coronavirus resistance in chickens [66], among others.…”

Macrophages from disease resistant B2 haplotype chickens activate T lymphocytes more effectively than macrophages from disease susceptible B19 birds

“…Unlike kidney cells, macrophages are considered professional antigen presenting cells, expressing both MHC I and MHC II and thus are capable of activating CD8 and CD4 T lymphocytes, respectively. AIV infection in chicken macrophages has been demonstrated previously (Xing et al, 2010;Barjesteh et al, 2014). Therefore, macrophages infected in vitro with AIV were ideal in evaluating differences in ex vivo activation of T lymphocytes purified from B2 and B19 birds vaccinated with the AIV NP.…”

“…Disease resistance to a variety of pathogens has been associated with MHC (major histocompatibility complex) haplotypes in several species, including mice and chickens (Banat et al, 2013;Briles and Briles, 1982;Dunnington et al, 1992;Heinzelmann et al, 1981;Joiner et al, 2007;Kim et al, 2008;Briles and Briles, 1987;Lamont, 1998;Mays et al, 2005;Mills, 2015;Yoo and Sheldon, 1992). In the chicken, MHC B haplotypes have been shown to display differential resistance to several viruses; including Marek's disease virus, avian leukosis virus, Newcastle disease virus, Rous sarcoma virus, infectious bronchitis virus (IBV) and avian influenza virus (AIV), as well as Salmonella (Banat et al, 2013;Briles and Briles, 1982;Lambrecht et al, 2004;Dunnington et al, 1992;Heinzelmann et al, 1981;Joiner et al, 2007;Kim et al, 2008;Lamont, 1998;Mays et al, 2005;Yoo and Sheldon, 1992).…”

“…Following infection with IBV, birds with the homozygous B2 haplotype have been shown to be more resistant to respiratory illness than B19 haplotype birds (Banat et al, 2013). Furthermore, B19 monocytes from peripheral blood mononuclear cells (PBMC) were slower to differentiate into macrophages upon in vitro culture than monocytes from the PBMC of birds with the B2 homozygous haplotype (Dawes et al, 2014).…”