Establishment of six homozygous MHC-B haplotype populations associated with susceptibility to Marek’s disease in Chinese specific pathogen-free BWEL chickens

Gao, Caixia; Han, Le; Han, Jianlin; Liu, Jiasen; Jiang, Qian; Guo, Dongchun; Qu, Liandong

doi:10.1016/j.meegid.2014.10.031

Cited by 11 publications

(10 citation statements)

References 56 publications

(90 reference statements)

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“…The observed allele combinations of seven MHC-B standard haplotypes of the ADOL line were consistent with their original sizes reported by Fulton et al [21] and the six homozygous MHC-B haplotypes described by Gao et al [58]. The haplotype MHC-B_MS7, which corresponds to the B21 standard haplotype, was observed in 15 populations.…”

Section: Haplotype Diversitysupporting

confidence: 89%

High Levels of Genetic Variation in MHC-Linked Microsatellite Markers from Native Chicken Breeds

Manjula

Kim²,

Cho³

et al. 2021

Genes

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The major histocompatibility complex (MHC) is a highly polymorphic gene region that regulates cellular communication in all specific immune responses. In this study, we investigated 11 microsatellite (MS) markers in the MHC-B region of chicken populations from four countries: Sri Lanka, Bangladesh, South Korea, and Nigeria. The MS markers were divided into two sets. Set 1 included five novel MS markers, which we assessed using 192 samples from 21 populations. Set 2 included six previously reported markers, which we assessed using 881 samples from 29 populations. The Set 1 MS markers had lower polymorphism (polymorphic information content (PIC) < 0.5) than the Set 2 markers (PIC = 0.4–0.9). In all populations, the LEI0258 marker was the most polymorphic, with a total of 38 alleles (PIC = 0.912, expected heterozygosity (He) = 0.918). Local populations from Sri Lanka, Bangladesh, and Nigeria had higher allele diversity and more haplotypes for Set 2 MS markers than Korean and commercial populations. The Sri Lankan Karuwalagaswewa village population had the highest MHC diversity (mean allele number = 8.17, He = 0.657), whereas the white leghorn population had the lowest (mean allele number = 2.33, He = 0.342). A total of 409 haplotypes (89 shared and 320 unique), with a range of 4 (Rhode Island red) to 46 (Karuwalagaswewa village (TA)), were identified. Among the shared haplotypes, the B21-like haplotype was identified in 15 populations. The genetic relationship observed in a neighbour-joining tree based on the DA distance agreed with the breeding histories and geographic separations. The results indicated high MHC diversity in the local chicken populations. The difference in the allelic pattern among populations presumably reflects the effects of different genotypes, environments, geographic variation, and breeding policies in each country. The selection of MHC allele in domestic poultry can vary due to intensification of poultry production. Preserved MHC diversity in local chicken provides a great opportunity for future studies that address the relationships between MHC polymorphisms and differential immune responses.

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Section: Haplotype Diversitysupporting

confidence: 89%

High Levels of Genetic Variation in MHC-Linked Microsatellite Markers from Native Chicken Breeds

Manjula

Kim²,

Cho³

et al. 2021

Genes

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“…Previous studies have shown the great potential of CATHL1 as an antiviral infectant [35], which is consistent with the results of this are essential for host-pathogen interactions and deliver both their own and external antigenic peptides to T lymphocytes [36]. Studies on MHCI in poultry mainly focus on the relationship between its haplotype and resistance [37][38][39]. In this study, the expression of MHCI protein in Tibetan chickens was lower than in Avian broilers and Pengxian yellow chickens, indicating that MHCI protein may not play a major role in anti-virus.…”

Section: Discussionsupporting

confidence: 73%

Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic

Wang

et al. 2020

Virulence

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Avian leucosis (AL) is a disease characterized by tumors and is caused by the avian leukosis virus (ALV). Because of the high variability of viruses and complex pathogenic mechanisms, screening and breeding J subgroup of ALV (ALV-J) resistant avian breeds is one of the strategies for prevention and treatment of AL, thus screening of significant immune markers is needed to promote the development of disease-resistant breeds. In this study, data-independent acquisition (DIA) technology was used to detect the DEPs of three breeds of chicken according to different comparison to investigate the potential markers. Results showed special DEPs for spleen development of each breed were detected, such as PCNT, DDB2, and ZNF62. These DEPs were involved in intestinal immune network used in production of IgA signaling pathways and related to immune response which can be used as potential markers for spleen development in different breeds. The DEPs such as RAB44 and TPN involved in viral myocarditis, transcriptional misregulation in cancer, and tuberculosis can be used as potential markers of spleen immune response after ALV-J infection in chickens. Pair-wise analysis was performed for the three breeds after the infection of ALV-J. The proteins such as RFX1, TAF10, and VH1 were differently expressed between three breeds. These DEPs involved in antigen processing and expression, acute myelogenous leukemia, and viral carcinogenesis can be used as potential immune markers after ALV-J infection of different genetic backgrounds. The screening of potential markers at protein level provides a strong theoretical research basis for disease resistance breeding in poultry.

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“…Three outbred groups and three haplotype groups of experimental chicken were used in this research: outbred group BWEL-SPF chickens ((SCXK (black) 2017-005)), 40 samples, 37 weeks old, 6 males and 34 females, which has been closed for 20 generations; outbred group BM chicken (from BWEL chicken lineage (SCXK (black) 2017-005)), 40 samples, 14 weeks old, 6 males and 34 females; outbred group Beijing oil chickens, 46 samples. MHC haplotype chickens were bred from the 13th generation of BWEL chicken, the haplotype was continuously selected based on the MHC core genes, and the half-sibling or sibling mating method was used to breed to the 8th generation [ 13 ]. We selected 5 G1 haplotype chickens, 53 weeks old, 1 male and 4 females; 5 G2 haplotype chickens, 93 weeks, 1 male and 4 females; and 5 G7 haplotype chickens, 82 weeks, 1 male and 4 females.…”

Section: Methodsmentioning

confidence: 99%

Development of Microsatellite Marker System to Determine the Genetic Diversity of Experimental Chicken, Duck, Goose, and Pigeon Populations

Zhang

et al. 2021

BioMed Research International

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Poultries including chickens, ducks, geese, and pigeons are widely used in the biological and medical research in many aspects. The genetic quality of experimental poultries directly affects the results of the research. In this study, following electrophoresis analysis and short tandem repeat (STR) scanning, we screened out the microsatellite loci for determining the genetic characteristics of Chinese experimental chickens, ducks, geese, and pigeons. The panels of loci selected in our research provide a good choice for genetic monitoring of the population genetic diversity of Chinese native experimental chickens, ducks, geese, and ducks.

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Establishment of six homozygous MHC-B haplotype populations associated with susceptibility to Marek’s disease in Chinese specific pathogen-free BWEL chickens

Cited by 11 publications

References 56 publications

High Levels of Genetic Variation in MHC-Linked Microsatellite Markers from Native Chicken Breeds

High Levels of Genetic Variation in MHC-Linked Microsatellite Markers from Native Chicken Breeds

Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic

Development of Microsatellite Marker System to Determine the Genetic Diversity of Experimental Chicken, Duck, Goose, and Pigeon Populations

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