Comparisons of Subgingival Microbial Profiles of Refractory Periodontitis, Severe Periodontitis, and Periodontal Health Using the Human Oral Microbe Identification Microarray

Colombo, Ana Paula Vieira; Boches, Susan K.; Cotton, Sean L.; Goodson, J. Max; Kent, Ralph; Haffajee, A. D.; Socransky, S. S.; Hastürk, Hatice; Dyke, Thomas E. Van; Dewhirst, Floyd E.; Paster, Bruce J.

doi:10.1902/jop.2009.090185

Cited by 482 publications

(588 citation statements)

References 58 publications

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“…Interestingly, in the present study also the activating vehicle itself exerted certain antimicrobial activity. This selective inhibition may favor a more Grampositive microbiota with Actinomyces spp., oral streptococci being in general more associated with periodontal health [24]. Moreover, it should be noted, that Actinomyces spp.…”

Section: Discussionmentioning

confidence: 99%

In-vitro activity of sodium-hypochlorite gel on bacteria associated with periodontitis

et al. 2016

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2Objectives: The aim of the present study was to assess the antimicrobial activity of a sodium hypochlorite formulation incl. its components against bacteria associated with periodontal disease. Materials and Methods:Sodium hypochlorite formulation (NaOCl gel), its components sodium hypochlorite (NaOCl) and the activating vehicle were compared with 0.1% chlorhexidine digluconate (CHX) solution. The antimicrobial activity was proven by determination of minimal inhibitory concentrations (MIC), minimal bactericidal concentrations, and killing assays. Furthermore, the influence on formation as well as on a 4-days old 6-species biofilm was tested.Results: Except for one strain (Parvimonas micra ATCC 33270 in case of NaOCl gel) the MICs both of the CHX solution and NaOCl gel did not exceed 10% of the formulations' concentration. In general MICs of the NaOCl gel were equal as of the CHX solution against Gram-negatives but higher against Gram-positive bacteria. CHX but not NaOCl gel clearly inhibited biofilm formation, however the activity of NaOCl gel was more remarkable on a 4-d old biofilm. NaOCl killed bacteria in the biofilm and interfered with the matrix. Conclusions:The NaOCl gel acts antimicrobial in particular against Gram-negative species associated with periodontitis. Moreover, its component NaOCl hypochlorite is able to alter biofilm matrices.Clinical relevance: The NaOCl gel may represent a potential alternative for adjunctive topical antimicrobial treatment in periodontitis

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Section: Discussionmentioning

confidence: 99%

In-vitro activity of sodium-hypochlorite gel on bacteria associated with periodontitis

et al. 2016

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“…Many of these microorganisms are associated with caries, periodontal and endodontic infections [69–72], but they have also been isolated from extra-oral infections or systemic conditions [52–54,73–75]. In contrast, S. australis and G. adiacens have been related to periodontal health [69,70]. …”

Section: Discussionmentioning

confidence: 99%

Defining the gut microbiota in individuals with periodontal diseases: an exploratory study

Lourenςo

Spencer

Alm

et al. 2018

Journal of Oral Microbiology

116

109

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Background: This exploratory study aimed to characterize the gut microbiome of individuals with different periodontal conditions, and correlate it with periodontal inflammation and tissue destruction.Methods: Stool samples were obtained from individuals presenting periodontal health (PH = 7), gingivitis (G = 14) and chronic periodontitis (CP = 23). The intestinal microbiome composition was determined by Illumina MiSeq sequencing.Results: A lower alpha-diversity in the gut microbiome of individuals with CP was observed, although no significant difference among groups was found (p > 0.01). Firmicutes, Proteobacteria, Verrucomicrobia and Euryarchaeota were increased, whereas Bacteroidetes were decreased in abundance in patients with periodontitis compared to PH. Prevotella (genus), Comamonadaceae (family) and Lactobacillales (order) were detected in higher numbers in G, while Bacteroidales (order) was predominant in PH (p < 0.01). Significant correlations (rho = 0.337–0.468, p < 0.01) were found between OTUs representative of periodontal pathogens and attachment loss. Mogibacteriaceae, Ruminococcaceae and Prevotella were able to discriminate individuals with periodontal diseases from PH (overall accuracy = 84%). Oral taxa were detected in high numbers in all stool samples.Conclusions: Individuals with periodontal diseases present a less diverse gut microbiome consistent with other systemic inflammatory diseases. High numbers of oral taxa related to periodontal destruction and inflammation were detected in the gut microbiome of individuals regardless of periodontal status.

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“…Early studies using culture-based techniques were labor-intensive (21), and many organisms remained uncultivable despite improvements in culturing techniques (22). Subsequent molecular approaches based on DNA-DNA hybridization and microarray techniques substantially improved our understanding of these microbial communities (23)(24)(25)(26)(27). However, since knowledge of nucleic acid signatures or hybridization probes are required a priori, these methods precluded detailed analyses of entire microbial communities.…”

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confidence: 99%

Dysbiosis and Alterations in Predicted Functions of the Subgingival Microbiome in Chronic Periodontitis

Kirst

Alfant

et al. 2015

Appl Environ Microbiol

186

188

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Chronic periodontitis is an inflammatory disease of the periodontium affecting nearly 65 million adults in the United States. Changes in subgingival microbiota have long been associated with chronic periodontitis. Recent culture-independent molecular studies have revealed the immense richness and complexity of oral microbial communities. However, data sets across studies have not been directly compared, and whether the observed microbial variations are consistent across different studies is not known. Here, we used 16S rRNA sequencing to survey the subgingival microbiota in 25 subjects with chronic periodontal disease and 25 healthy controls and compared our data sets with those of three previously reported microbiome studies. Consistent with data from previous studies, our results demonstrate a significantly altered microbial community structure with decreased heterogeneity in periodontal disease. Comparison with data from three previously reported studies revealed that subgingival microbiota clustered by study. However, differences between periodontal health and disease were larger than the technical variations across studies. Using a prediction score and applying five different distance metrics, we observed two predominant clusters. One cluster was driven by Fusobacterium and Porphyromonas and was associated with clinically apparent periodontitis, and the second cluster was dominated by Rothia and Streptococcus in the majority of healthy sites. The predicted functional capabilities of the periodontitis microbiome were significantly altered. Genes involved in bacterial motility, energy metabolism, and lipopolysaccharide biosynthesis were overrepresented in periodontal disease, whereas genes associated with transporters, the phosphotransferase system, transcription factors, amino acid biosynthesis, and glycolysis/gluconeogenesis were enriched in healthy controls. These results demonstrate significant alterations in microbial composition and function in periodontitis and suggest genes and metabolic pathways associated with periodontal disease. P eriodontal disease is a common disease affecting many adults in the United States. If left untreated, chronic periodontitis (CP) can lead to serious problems such as tooth loss. Indeed, periodontal disease is the number one cause of tooth loss in the United States, accounting for half of all tooth loss in U.S. adults (1). There is considerable evidence that the clinical impact of periodontal disease extends beyond the oral cavity (2). Strong associations have been found between periodontitis and a number of systemic conditions, including cardiovascular disease (3, 4), preterm delivery and low-birth-weight babies (5), diabetes mellitus (6-8), and rheumatoid arthritis (9-11).The activity of periodontal disease is determined by a complex interplay between the immune system and periodontal pathogens (12, 13). Alterations in subgingival microbiota have long been associated with the development and progression of periodontitis (14). In susceptible individuals, perturbations in...

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Comparisons of Subgingival Microbial Profiles of Refractory Periodontitis, Severe Periodontitis, and Periodontal Health Using the Human Oral Microbe Identification Microarray

Cited by 482 publications

References 58 publications

In-vitro activity of sodium-hypochlorite gel on bacteria associated with periodontitis

In-vitro activity of sodium-hypochlorite gel on bacteria associated with periodontitis

Defining the gut microbiota in individuals with periodontal diseases: an exploratory study

Dysbiosis and Alterations in Predicted Functions of the Subgingival Microbiome in Chronic Periodontitis

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