Comparisons between fresh and cryopreserved fat injections in facial lipofilling

Yang, Hyee Jae; Kang, Sun-Woong

doi:10.7181/acfs.2019.00612

Cited by 4 publications

(7 citation statements)

References 34 publications

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“…Studies both in vitro and in vivo were conducted to assess whether the cold was toxic to the adipocytes and whether cell survival was affected by decreasing the storage temperature from 4°C to −80°C, but yet to provide a uniform conclusion. [10][11][12][13][14] Some studies indicate that there were no significant differences between the fresh and cryopreserved fat injections. 14,15 While in other studies, the results are different.…”

Section: Discussionmentioning

confidence: 99%

“…[10][11][12][13][14] Some studies indicate that there were no significant differences between the fresh and cryopreserved fat injections. 14,15 While in other studies, the results are different. Melike et al 10 have demonstrated that the viability of fat grafts stored at 4°C provides the highest number of viable adipocytes compared with dry, frozen samples (−20°C and −80°C) for at least 2 weeks.…”

Section: Discussionmentioning

confidence: 99%

“…Further data on the response of adipocytes to cold were derived from studies focused on whether autologous fat grafts could maintain the best quality for transplantation when they were fresh, refrigerated, or frozen. Studies both in vitro and in vivo were conducted to assess whether the cold was toxic to the adipocytes and whether cell survival was affected by decreasing the storage temperature from 4°C to −80°C, but yet to provide a uniform conclusion 10–14 . Some studies indicate that there were no significant differences between the fresh and cryopreserved fat injections 14,15 .…”

Section: Discussionmentioning

confidence: 99%

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The Effect of Various Temperatures on the Inflammatory Profile of Fat Graft Storage: An Experimental Study

Yuan

et al. 2023

Journal of Craniofacial Surgery

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Fat tissue has been widely used as a filler material during plastic surgery, but unpredictable fat retention remains a significant concern. Fat tissue is vulnerable to ischemia and hypoxia, but it always has waiting time before injection in the operation theater. Apart from transferring fat tissue as quickly as possible after harvesting, washing the aspirate with cool normal saline is often used. However, the mechanisms of cool temperature acting on adipose tissue have yet to be fully elucidated. Herein, this study aims to explore the effect of preservation at different temperatures on the inflammatory profile of adipose tissue. Inguinal adipose tissue of rats was collected and cultured in vitro under 4°C, 10°C, and room temperature for 2 hours. The proportion of damaged adipocytes and an array of cytokines were determined. We observed that the damage rate of the adipocyte membrane was slightly higher at room temperature, but there was no significant difference, while we noticed increased IL-6 and MCP-1 levels in adipose tissue at room temperature (P＜0.01). The 4°C and 10°C cool temperatures may offer protection against proinflammatory states during the adipose tissue preserved in vitro.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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The Effect of Various Temperatures on the Inflammatory Profile of Fat Graft Storage: An Experimental Study

Yuan

et al. 2023

Journal of Craniofacial Surgery

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“…However, AFI’s main obstacle in augmentation is an unpredictable outcome from a high absorption rate and necrosis at the grafted site [ 14 ]. For acute inflammation after fat grafting, good results can be obtained by re-transplanting fat after treatment for inflammation [ 15 ]. However, in this case of patients who visited the hospital due to persistent chronic inflammation, it was judged that the possibility of recurrence would be high if the fat was re-transplanted.…”

Section: Discussionmentioning

confidence: 99%

Evaluation and management of facial granuloma caused by various injection materials

Jang

Kang

2021

Arch Craniofac Surg

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Background Various substances are currently being used for cosmetic or postsurgical reconstruction reasons. Injecting of various materials into the face may provoke inflammatory or granulomatous reactions, infection and tissue necrosis. Among these reactions are foreign body granulomas. This study aims to describe the clinical characteristics of and therapeutic approaches used in patients with facial foreign body granulomas caused by the injection of various substances. Methods From 2007 to 2020, a total of 64 patients visited our hospital due to inflammatory signs, palpable masses or surface irregularities. We reviewed patient characteristics, type of injected material, medical history and treatment outcomes. The treatment results were scored with patient satisfaction and statistical analysis of the treatment period was performed. Results Most patients underwent conservative treatment followed by surgical treatment because of persistent symptoms; one patient did not require surgery. All patients reported good treatment results and satisfaction. The earlier the surgical treatment was performed, the shorter the treatment period and the higher patient satisfaction scoring. Conclusion Granulomatous changes to the face are an emerging concern in various cosmetic procedures and surgeries, including fillers, silicone implants and autologous fat injections. Our findings strongly suggest that patient symptoms require accurate diagnosis and surgical treatment.

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“…To date, however, no study has reported the effect of frozen fat grafters on the rate of volume retention during longer follow-up periods [ 8 , 9 ]. Moreover, frozen fat face injection has been found to have a similar clinical effect to fresh facial fat injection [ 10 ]. Cryopreservation of adipose tissue is accompanied by various problems, key among them cooling and thawing temperatures, as well as the use of cryopreservation agents [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

SVF-GEL Cryopreserved for Different Times Exhibits Varied Preservation and Regeneration Potential After Transplantation in a Mouse Model

et al. 2022

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Background Matrix vascular component (SVF) gels derived from fat preserve tissue integrity and cell viability under cryopreserved conditions, making them easy to inject again for later use. Here, we compared the preservation power and regeneration potential of SVF-gel under different cryopreservation times. Methods The SVF-gel stored under − 20 °C, without cryoprotectant cryopreservation for 5, 15, and 45 days, with fresh SVF-gel as control. We evaluated the rate of volume retention after thawing the SVF-gel and the apoptosis rate of adipose-derived stem cells. Next, we analyzed retention rated, adipogenesis, angiogenesis, and connective tissue hyperplasia of the grafts, one month after subcutaneously transplanting the specimen into immunodeficient mice. Results SVF-gel cryopreserved for 5 and 15 days exhibited no significant different in apoptosis rates relative to the control group. Extending the cryopreservation time to 45 days resulted in significantly increased and decreased apoptosis and volume retention rates of SVF-gel, respectively. SVF-gel grafts cryopreserved for 5 and 15 days exhibited no significant differences from those in the control group, although their weights and volumes still fluctuated. Extending the cryopreservation time to 45 days resulted in significantly decreased retention rates of the grafts. Histologically, extending freezing time resulted in a gradual decline in the graft’s health adipose tissue, as well as decreased angiogenesis, and connective tissue hyperplasia. Conclusion Simple freezing of SVF-gel at − 20 °C conferred them with sufficient cell viability. Notably, short-term cryopreservation did not significantly increase the apoptosis rate, and it still had a certain regeneration after transplantation. However, prolonging freezing time to 45 days resulted in increased apoptosis rate and worsened transplantation effect. No Level Assigned This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

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Comparisons between fresh and cryopreserved fat injections in facial lipofilling

Cited by 4 publications

References 34 publications

The Effect of Various Temperatures on the Inflammatory Profile of Fat Graft Storage: An Experimental Study

The Effect of Various Temperatures on the Inflammatory Profile of Fat Graft Storage: An Experimental Study

Evaluation and management of facial granuloma caused by various injection materials

SVF-GEL Cryopreserved for Different Times Exhibits Varied Preservation and Regeneration Potential After Transplantation in a Mouse Model

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