Comparison, synthesis and evaluation of anticancer drug-loaded polymeric nanoparticles on breast cancer cell lines

Abstract

Breast cancer is a major form of cancer, with a high mortality rate in women. It is crucial to achieve more efficient and safe anticancer drugs. Recent developments in medical nanotechnology have resulted in novel advances in cancer drug delivery. Cisplatin, doxorubicin, and 5-fluorouracil are three important anti-cancer drugs which have poor water-solubility. In this study, we used cisplatin, doxorubicin, and 5-fluorouracil-loaded polycaprolactone-polyethylene glycol (PCL-PEG) nanoparticles to improve the stability and solubility of molecules in drug delivery systems. The nanoparticles were prepared by a double emulsion method and characterized with Fourier Transform Infrared (FTIR) spectroscopy and Hydrogen-1 nuclear magnetic resonance ((1)HNMR). Cells were treated with equal concentrations of cisplatin, doxorubicin and 5-fluorouracil-loaded PCL-PEG nanoparticles, and free cisplatin, doxorubicin and 5-fluorouracil. The 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl tetrazolium bromide (MTT) assay confirmed that cisplatin, doxorubicin, and 5-fluorouracil-loaded PCL-PEG nanoparticles enhanced cytotoxicity and drug delivery in T47D and MCF7 breast cancer cells. However, the IC50 value of doxorubicin was lower than the IC50 values of both cisplatin and 5-fluorouracil, where the difference was statistically considered significant (p˂0.05). However, the IC50 value of all drugs on T47D were lower than those on MCF7.

Nanoscale modification of chrysin for improved of therapeutic efficiency and cytotoxicity

“…Several reports in recent years showed that chrysin is a candidate for cancer therapy in different kinds of cancer cell lines due to it is activity to inhibit the cell proliferation and induction of cell death via apoptosis pathway [11,12]. Although, chrysin possesses anticancer properties, its poor water solubility limited its biocompatibility, bioavailability and biomedical applications, indicating an important problem for cancer treatment [13] because of quick metabolism, low absorption and rapid systemic elimination [2]. One of the approaches to overcome this problem is the use of nanoparticles.…”

Effects of nano-encapsulated curcumin-chrysin on telomerase, MMPs and TIMPs gene expression in mouse B16F10 melanoma tumour model

“…To resolve this problem, nanotechnology has offered water soluble and biocompatible NPs, which their physicochemical properties make them good choices to deliver various types of the anti-cancer drugs [22]. Dissolving drugs-loaded NPs in water results in the hydrophilic shell and hydrophobic core for stabilization of NP and loaded drug, respectively [23]. On the other hand, encapsulating drugs in engineered NPs can improve the safety of drug delivery and enable directed drug delivery to target tissues without damages to the healthy ones [24,25].…”

Effect of Artemisia Aucheri.L and Bupivacaine Encapsulated Nanoparticles on Nociceptive Pain

“…Artemisia aucheri-loaded magnetic hydrogel nanocomposite was fabricated through hydrogel dispersion in NaOH in the presence of magnetic nanoparticles (MNPs) and Artemisia aucheri extract [18]. Fifty (50 mg) of extract and 100 mg of MNPs were dispersed in 40 ml of 25 mM NaOH solution, the solution is sonicated at 0.5 cycle, 50 % amplitude for 15 min.…”