Comparison of γ-radiation and electron beam irradiation effects on gelatin

Vieira, Fernando Fernandes; Mastro, Nélida L. del

doi:10.1016/s0969-806x(01)00522-9

Cited by 39 publications

(16 citation statements)

References 3 publications

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“…The reduction of the average molecular weight of the hard shell components, 3,5,6 confirmed by the [η] reduction and the alteration in the AFM distance-force curves, did not affect the technological performance of the irradiated capsules at doses lower than or equal to 25 kGy.…”

Section: Technological Performancesmentioning

confidence: 89%

“…The viscosity of irradiated solutions containing bovine powder gelatin was significantly affected by both β-or γ-rays when doses higher than 5 kGy were administered. 3 The effects of γ-irradiation on HPMC raw material were studied by calorimetry, spectrometry, rheology, 4 electronic paramagnetic resonance analysis and capillary viscometry. 5 In these studies it has been demonstrated that the main radiolytic process is based on HPMC chain scission events.…”

Section: Introductionmentioning

confidence: 99%

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Comparison between gamma and beta irradiation effects on hydroxypropylmethylcellulose and gelatin hard capsules

et al. 2005

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The effects of electron beam or γ-irradiation on technological performances (capsule hardness, expressed as deforming work and dissolution time) of empty 2-shell capsules made of gelatin or hydroxypropylmethylcellulose (HPMC) were studied. Capsule structural changes induced by radiation treatment were investigated by capillary viscometry and atomic force microscopy (AFM). The capsules were irradiated in the air at 5, 15, and 25 kGy. The deforming work of nonirradiated HPMC capsules (0.06 ± 0.01 J) was lower than that of gelatin capsules (0.10 ± 0.01 J). The dissolution time of the HPMC capsules (414 ± 33 seconds) was slightly higher than that determined for gelatin hard capsules (288 ± 19 seconds). The hardness and dissolution time of gelatin and HPMC capsules were not significantly influenced by the irradiation type and the applied irradiation dose. As the viscometry analyses are concerned, irradiation caused a reduction of the intrinsic viscosity and water and dimethyl sulfoxide solvent power in both the cases. AFM analysis showed that the radiation treatment did not appreciably affect the surface roughness of the samples nor induce structural changes on capsule surface. However, measurements of force-distance curves pointed out a qualitative parameter for the identification of the irradiated capsules. On the bases of these preliminary results, empty gelatin or HPMC hard capsules can be sanitized/ sterilized by ionizing radiation.

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Section: Technological Performancesmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Comparison between gamma and beta irradiation effects on hydroxypropylmethylcellulose and gelatin hard capsules

et al. 2005

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“…In recent years there has been an increasing interest in the use of collagen and its derivatives as biomaterials [1][2][3][4][5]. Complete physical and electrical characterized ions are essential to define the extent and limit of their applicability.…”

Section: Introductionmentioning

confidence: 99%

Drastic effects of fast neutrons and γ-irradiation on the DC conductivity of Co-, Ni-, Mn- and Ag-gelatin doped films

Hanafy

2008

Current Applied Physics

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“…Gamma-irradiation induced mainly the scission of the collagen subunit in the dry state [4,5], while it induced cross-linking of the subunits rather than the scission in the wet state [4, 6 -8]. The scission process has been investigated in order to keep the mechanical strength of tissues such as bone, tendon and ligament as allografts for transplants during the sterilization by γ -rays [9].…”

Section: Introductionmentioning

confidence: 99%

A novel collagen hydrogel cross-linked by gamma-ray irradiation in acidic pH conditions

Inoue

Bessho²,

Furuta³

et al. 2006

Journal of Biomaterials Science, Polymer Edition

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We made a new type of collagen gel by gamma-ray irradiation of an acidic solution of type-I collagen, and performed comparative studies on a conventional gel and the new type of gel. The neutral gel, a conventional 0.3% (w/v) collagen gel, was formed at neutral pH and then irradiated by gamma-rays. The acidic gel, a 0.3% (w/v) collagen gel, was formed directly from the acidic solution of collagen by y-ray irradiation. Both types of gel were prepared, swollen in water and then dried for the measurement of specific water content. The neutral gel showed a relatively high specific water content and shrunk moderately, depending on the dose, while the acidic gel showed lower specific water content and shrunk clearly by y-ray irradiation. A three-dimensional tangled network of microfibrils was clearly observed in the neutral gels by scanning electron microscopy, but not in the acidic gels. From these results, we concluded that the acidic gel was quite different from a conventional collagen gel. Sodium dodecylsulfate-polyacrylamide gel electrophoresis showed that the alpha1 subunit and alpha2 subunit of the collagen molecule were cross-linked. The triple-helical structure of collagen was only partially perturbed, but not denatured completely, because the circular dichroism spectrum of the collagen solution irradiated at 1.3 kGy was similar to that of native collagen solution. Amino-acid analysis revealed that tyrosine, phenylalanine and histidine decreased by irradiation in the neutral gel. In the case of the acidic gel, these three amino acids and methionine decreased. We considered that these amino acids were cross-linking points between the collagen subunits during the gamma-ray irradiation.

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Comparison of γ-radiation and electron beam irradiation effects on gelatin

Cited by 39 publications

References 3 publications

Comparison between gamma and beta irradiation effects on hydroxypropylmethylcellulose and gelatin hard capsules

Comparison between gamma and beta irradiation effects on hydroxypropylmethylcellulose and gelatin hard capsules

Drastic effects of fast neutrons and γ-irradiation on the DC conductivity of Co-, Ni-, Mn- and Ag-gelatin doped films

A novel collagen hydrogel cross-linked by gamma-ray irradiation in acidic pH conditions

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