Comparison of Xpert MRSA/SA Nasal and MRSA/SA ELITe MGB Assays for Detection of the
            <i>mecA</i>
            Gene with Susceptibility Testing Methods for Determination of Methicillin Resistance in Staphylococcus aureus Isolates

Belmekki, Mohamed; Mammeri, Hedi; Hamdad, Farida; Rousseau, Florence; Canarelli, B.; Biendo, M.

doi:10.1128/jcm.00860-13

Cited by 15 publications

(12 citation statements)

References 24 publications

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“…In the present study, the isolates were phenotypically resistant to all β-lactams but remained susceptible to other tested antibiotics and thus corresponded chromosomally to the mecC gene. Our results are in agreement with the literature data [17, 37–40]. When considering the genotyped strains, resistant strains ( B. cereus and E. faecalis ) were only found in the CPF groups (both CPF1 and CPF5)—particularly in juvenile rats at D21.…”

Section: Discussionsupporting

confidence: 93%

“…Resistance to macrolide, lincosamide, SA (PT) and SB (virginiamycin) is associated with the production of an SA-inactivating mutant acetyltransferase and an SB-inactivating mutant hydrolase [34–36]. Expression of the MRSA phenotype and susceptibility to other antibiotics has been documented in the literature [17, 37–40]. …”

Section: Discussionmentioning

confidence: 99%

“…A MALDI–TOF-MS system (Autoflex III, Bruker Daltonics, Billerica, MA, USA) was used to examine each unique colony of microorganisms isolated by culture, according to a previously described procedure [17–19]. In brief, samples were prepared with absolute ethanol, and 1 μL of matrix solution (2,5-dihydroxybenzoic acid 50 mg/mL, 30 % acetonitrile, 0.1 % trifluoroacetic acid) was added.…”

Section: Methodsmentioning

confidence: 99%

“…The frequency of BT was quoted with its 95 % confidence interval (CI, calculated using the “minlike” method) [17]. A mixed logistic regression model was used to determine the relationships between the likelihood of BT on one hand and exposure and age on the other.…”

Section: Methodsmentioning

confidence: 99%

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Use of molecular typing to investigate bacterial translocation from the intestinal tract of chlorpyrifos-exposed rats

Condette¹,

Dzon²,

Hamdad³

et al. 2016

Gut Pathog

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BackgroundHuman are confronted on a daily basis with contaminant pesticide residues in food, water and other components of the environment. Although the digestive system is the first organ to come into contact with food contaminants, very few data are available on the impact of low-dose pesticide exposure during the in utero and postnatal periods on intestinal bacterial translocation (BT). Previous studies have revealed that chlorpyrifos (CPF) exposure is associated with intestinal dysbiosis and the contamination of sterile organs. Here, molecular typing was used to investigate intestinal bacterial translocation in rats exposed to chlorpyrifos in utero and during lactation. The translocated bacteria were profiled, and CPF tolerance and antibiotic resistance traits were determined.MethodsA total of 72 intestinal segments and extra-intestinal organs were obtained from 14 CPF-exposed rats. The samples were cultured to isolate bacterial strains that had tolerated treatment with 1 or 5 mg CPF/kg bodyweight/day in vivo. Strains were identified using matrix-assisted laser desorption/ionization (MALDI) Biotyper. The disk diffusion method was used to determine the antibiotic susceptibility. The isolates were genotyped with PCR assays for the enterobacterial repetitive intergenic consensus sequence and random amplification polymorphic DNA.ResultsBacterial translocation was confirmed for 7 of the 31 strains (22.6 %) isolated from extra-intestinal sites. Overall, the most prevalent bacteria were Staphylococcus aureus (55.5 % of the 72 intestinal and extra-intestinal isolates), Enterococcus faecalis (27.7 %) and Bacillus cereus (9.8 %). 5 % of the S. aureus isolates displayed methicillin resistance. Seventy two strains were identified phenotypically, and seven translocated strains (mainly S. aureus) were identified by genotyping. Genotypically confirmed translocation was mainly observed found in pesticide-exposed groups (6 out of 7).ConclusionBT from the intestinal tract colonized normally sterile extra-intestinal organs in CPF-exposed rats. Our findings validate the use of molecular typing for the assessment of intestinal BT in CPF-exposed rats during critical periods of development.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Use of molecular typing to investigate bacterial translocation from the intestinal tract of chlorpyrifos-exposed rats

Condette¹,

Dzon²,

Hamdad³

et al. 2016

Gut Pathog

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“…mecC MRSA strains are relatively rare; their prevalence rate among MRSA isolates is 0.06% in Germany (141) and 0.46% in England (142) but reached 2.8% in Denmark in 2011, having increased since 2009 (143). Commercial and in-house PCR assays are being modified to allow simultaneous detection of mecA and mecC MRSA (139,(144)(145)(146)(147), and their epidemiology will be better understood as more laboratories test for mecC.…”

Section: Non-meca Gene-mediated Methicillin Resistancementioning

confidence: 99%

Mechanisms of Methicillin Resistance in Staphylococcus aureus

Peacock

Paterson

2015

Annu. Rev. Biochem.

490

371

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Staphylococcus aureus is a major human and veterinary pathogen worldwide. Methicillin-resistant S. aureus (MRSA) poses a significant and enduring problem to the treatment of infection by such strains. Resistance is usually conferred by the acquisition of a nonnative gene encoding a penicillin-binding protein (PBP2a), with significantly lower affinity for β-lactams. This resistance allows cell-wall biosynthesis, the target of β-lactams, to continue even in the presence of typically inhibitory concentrations of antibiotic. PBP2a is encoded by the mecA gene, which is carried on a distinct mobile genetic element (SCCmec), the expression of which is controlled through a proteolytic signal transduction pathway comprising a sensor protein (MecR1) and a repressor (MecI). Many of the molecular and biochemical mechanisms underlying methicillin resistance in S. aureus have been elucidated, including regulatory events and the structure of key proteins. Here we review recent advances in this area.

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Evaluation of the Cepheid Xpert SA Nasal Complete for direct detection of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus in nasal swabs from pediatric patients

Wang

Salamon

Jean

et al. 2021

Diagnostic Microbiology and Infectious Disease

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Comparison of Xpert MRSA/SA Nasal and MRSA/SA ELITe MGB Assays for Detection of the mecA Gene with Susceptibility Testing Methods for Determination of Methicillin Resistance in Staphylococcus aureus Isolates

Cited by 15 publications

References 24 publications

Use of molecular typing to investigate bacterial translocation from the intestinal tract of chlorpyrifos-exposed rats

Use of molecular typing to investigate bacterial translocation from the intestinal tract of chlorpyrifos-exposed rats

Mechanisms of Methicillin Resistance in Staphylococcus aureus

Evaluation of the Cepheid Xpert SA Nasal Complete for direct detection of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus in nasal swabs from pediatric patients

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