A novel hematology analyzer for small animal medicine, ProCyte Dx, was developed from
combination of the fluorescence laser flow cytometry and laminar flow impedance
technologies, and its accuracy was evaluated by comparing with the conventional
impedance-based hematology analyzer, pocH-100iV Diff, or microscopic manual cell counting
methods with staining blood smears in the canine blood. Blood samples of 59 dogs were
hematologically analyzed and compared by Pearson’s correlation coefficients. Analyses
between the two analyzers showed excellent correlation in RBC (r=0.998), HGB (r=0.999),
HCT (r=0.998), MCV (r=0.994), MCH (r=0.974), MCHC (r=0.906), WBC (r=0.998) and PLT
(r=0.993). Analyses between ProCyte Dx and microscopic manual counting results showed
excellent correlation in neutrophils (r=0.920), lymphocytes (r=0.913) and reticulocyte
percentages (r=0.924), good correlation in eosinophils (r=0.815) and reticulocyte numbers
(r=0.850) and fair correlation in monocytes (r=0.770). The present study indicates that
ProCyte Dx is acceptably accurate and can be a powerful tool for canine clinical
medicine.