Comparison of white blood cell differential percentages determined by the in‐house LaserCyte hematology analyzer and a manual method

Papasouliotis, Kostas; Cue, SM; Crawford, Elsa; Pinches, Mark; Dumont, Michel; Burley, Ken

doi:10.1111/j.1939-165x.2006.tb00134.x

Cited by 43 publications

(42 citation statements)

References 11 publications

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“…3). Similar analyses on leukogram were previously reported in LaserCyte (IDEXX Laboratories) which was made up of laser flow cytometry [8]. Making a comparison between the present and previous studies, all analytes in leukogram on ProCyte Dx could provide better results than those on LaserCyte (neutrophils, r =0.55; lymphocytes, r =0.76; monocytes, r =-0.05; eosinophils, r =0.60) in canine blood [8].…”

supporting

confidence: 81%

“…Similar analyses on leukogram were previously reported in LaserCyte (IDEXX Laboratories) which was made up of laser flow cytometry [8]. Making a comparison between the present and previous studies, all analytes in leukogram on ProCyte Dx could provide better results than those on LaserCyte (neutrophils, r =0.55; lymphocytes, r =0.76; monocytes, r =-0.05; eosinophils, r =0.60) in canine blood [8]. Although ProCyte Dx is thought to be a better analyzer, careful observation of blood preparations should be required, especially in cases with hematological abnormality, since leukogram by ProCyte Dx provides excellent results as the present study but may not be perfect, and morphological abnormality of blood cells cannot be recognized by the data.…”

supporting

confidence: 72%

“…All analyses were performed by using Microsoft Excel 2003 software (Microsoft Corp., Redmond, WA, U.S.A.). Based on an objective classification system for evaluation employed in previous studies [2, 3, 8, 9], correlation was characterized with modification as excellent ( r >0.90), good ( r =0.80 to 0.90), fair ( r =0.60 to<0.80) or poor ( r <0.60).…”

mentioning

confidence: 99%

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Development and Evaluation of a Novel In-Clinic Automated Hematology Analyzer, ProCyte Dx, for Canine Erythrocyte Indices, Leukogram, Platelet Counts and Reticulocyte Counts

et al. 2013

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A novel hematology analyzer for small animal medicine, ProCyte Dx, was developed from combination of the fluorescence laser flow cytometry and laminar flow impedance technologies, and its accuracy was evaluated by comparing with the conventional impedance-based hematology analyzer, pocH-100iV Diff, or microscopic manual cell counting methods with staining blood smears in the canine blood. Blood samples of 59 dogs were hematologically analyzed and compared by Pearson’s correlation coefficients. Analyses between the two analyzers showed excellent correlation in RBC (r=0.998), HGB (r=0.999), HCT (r=0.998), MCV (r=0.994), MCH (r=0.974), MCHC (r=0.906), WBC (r=0.998) and PLT (r=0.993). Analyses between ProCyte Dx and microscopic manual counting results showed excellent correlation in neutrophils (r=0.920), lymphocytes (r=0.913) and reticulocyte percentages (r=0.924), good correlation in eosinophils (r=0.815) and reticulocyte numbers (r=0.850) and fair correlation in monocytes (r=0.770). The present study indicates that ProCyte Dx is acceptably accurate and can be a powerful tool for canine clinical medicine.

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supporting

confidence: 81%

supporting

confidence: 72%

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Development and Evaluation of a Novel In-Clinic Automated Hematology Analyzer, ProCyte Dx, for Canine Erythrocyte Indices, Leukogram, Platelet Counts and Reticulocyte Counts

et al. 2013

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“…The differences may also relate to striking interspecies differences in neutrophil content of blood, since neutrophils represent only a relatively very small proportion, 10–25%, of leucocytes in mice, whereas neutrophils are far more prevalent in dog and human blood, where they represent about 70% and 50–70% (respectively) of leucocytes [102], [103]. This is an important point since the much higher neutrophil levels in dogs (and humans), and the 35 fold increase of neutrophils in GRMD muscles, implies a much greater role for the adverse effects of neutrophils in the severity of dystropathology in dogs and humans.…”

Section: Discussionmentioning

confidence: 99%

Levels of inflammation and oxidative stress, and a role for taurine in dystropathology of the Golden Retriever Muscular Dystrophy dog model for Duchenne Muscular Dystrophy

et al. 2016

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Duchenne Muscular Dystrophy (DMD) is a fatal skeletal muscle wasting disease presenting with excessive myofibre necrosis and increased inflammation and oxidative stress. In the mdx mouse model of DMD, homeostasis of the amino acid taurine is altered, and taurine administration drastically decreases muscle necrosis, dystropathology, inflammation and protein thiol oxidation. Since the severe pathology of the Golden Retriever Muscular Dystrophy (GRMD) dog model more closely resembles the human DMD condition, we aimed to assess the generation of oxidants by inflammatory cells and taurine metabolism in this species. In muscles of 8 month GRMD dogs there was an increase in the content of neutrophils and macrophages, and an associated increase in elevated myeloperoxidase, a protein secreted by neutrophils that catalyses production of the highly reactive hypochlorous acid (HOCl). There was also increased chlorination of tyrosines, a marker of HOCl generation, increased thiol oxidation of many proteins and irreversible oxidative protein damage. Taurine, which functions as an antioxidant by trapping HOCl, was reduced in GRMD plasma; however taurine was increased in GRMD muscle tissue, potentially due to increased muscle taurine transport and synthesis. These data indicate a role for HOCl generated by neutrophils in the severe dystropathology of GRMD dogs, which may be exacerbated by decreased availability of taurine in the blood. These novel data support continued research into the precise roles of oxidative stress and taurine in DMD and emphasise the value of the GRMD dogs as a suitable pre-clinical model for testing taurine as a therapeutic intervention for DMD boys.

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“…It is available with multiple preprogrammed settings for the commonest domestic species. Although comparative studies have been conducted between automated hematology systems and manual techniques using samples of most of the domestic species Science Publications AJAVS (Hennessy et al, 1998;Bienzle et al, 2000;Dawson et al, 2000;Papasouliotis et al, 2006;Roleff et al, 2007), such a comparison has not been made for ovine blood except for a comparison of blood samples from lambs collected into two types of vacutainer tubes, one containing EDTA and the other heparin (Vatn et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study of the Ovine Haemogram: Cell-Dyn 3500 Versus Manual Methods

Athanasiou¹

2013

American Journal of Animal and Veterinary Sciences

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Manual hematology techniques traditionally applied in farm animal medicine are time consuming and laborintensive, especially when large numbers of samples have to be processed. As a result, several automated hematology instruments have been developed for use in these species. An automated hematology analyzer (Abbott Cell-Dyne 3500 system) was used in performing a complete blood cell count and differential counts of white blood cells in sheep blood samples. The system was compared with basic manual hematologic techniques. A linear regression was used to assess correlation between the two methods. Correlation coefficients (R2) were good for the hematocrit, the total white blood cell count, the neutrophils, the lymphocytes and the platelet count, while a poor correlation existed in monocytes, eosinophils and basophils. The automated and the manual technique were also compared in terms of sensitivity using the Sensitivity Ratio (SR). The automated analyzer was slightly more sensitive than the manual technique for all parameters tested except for monocytes, eosinophils and basophils were the difference was greater and the automated analyzer was 1.5, 2.5 and 2 times more reliable, respectively. The method bias was also calculated. It seems that the overall performance of the automated analyzer justifies its utilization in sheep blood analysis, although as for any analyzer used in any species, a stained blood film evaluation remains an indispensable technique to confirm the results being reported by the automated analyzer and provide additional information for the ovine haemogram.

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Comparison of white blood cell differential percentages determined by the in‐house LaserCyte hematology analyzer and a manual method

Abstract: Although use of a 100-cell (vs 200-cell) M-diff% may be a limitation of our study, good correlation between WBC differentials obtained using the LaserCyte and the manual method was achieved only for feline eosinophils.

Cited by 43 publications

References 11 publications

Development and Evaluation of a Novel In-Clinic Automated Hematology Analyzer, ProCyte Dx, for Canine Erythrocyte Indices, Leukogram, Platelet Counts and Reticulocyte Counts

Development and Evaluation of a Novel In-Clinic Automated Hematology Analyzer, ProCyte Dx, for Canine Erythrocyte Indices, Leukogram, Platelet Counts and Reticulocyte Counts

Levels of inflammation and oxidative stress, and a role for taurine in dystropathology of the Golden Retriever Muscular Dystrophy dog model for Duchenne Muscular Dystrophy

A Comparative Study of the Ovine Haemogram: Cell-Dyn 3500 Versus Manual Methods

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