Comparison of voltammetric detection assisted by multivariate curve resolution with amperometric detection in liquid chromatographic analysis of cysteine-containing compounds

González-García, Olga; Ariño, Cristina; Dı́az-Cruz, José Manuel; Esteban, Miquel

doi:10.1016/j.chroma.2004.11.018

Cited by 22 publications

(9 citation statements)

References 28 publications

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“…These results suggested that the developed method for the determination of the six analytes was accurate and sensitive. Compared with other reported methods [27][28][29][30][31], similar response ranges but higher detection limits were achieved with the HPLC-ELSD method compared with the HPLC-fluorescence (Flu) method. However, the HPLC-Flu method has a complicated derivatization process, whereas low detection limits can be obtained with the HPLC-ELSD method relative to the HPLC-ED or ion-pair chromatography (IPC)-MS methods (table 2).…”

Section: Calibration Curves Linear Ranges and Detection Limitsmentioning

confidence: 68%

Development of the HPLC–ELSD method for the determination of phytochelatins and glutathione inPerilla frutescensunder cadmium stress conditions

et al. 2018

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A rapid, accurate and simple method was developed for the simultaneous determination of glutathione (GSH) and phytochelatins (PCs) by high-performance liquid chromatography (HPLC) with an evaporative light-scattering detector. GSH, phytochelatin 2 (PC2), PC3, PC4, PC5 and PC6 can be separated with baseline separation within 9 min using a Venusil AA column (250 mm × 4.6 mm i.d., 5 µm particle sizes). Acetonitrile and water containing 0.1% trifluoroacetic acid (0.1%) were employed as the mobile phase for the gradient elution. The drift tube temperature and flow rate of the carrier gas (N2) were 50°C and 1.5 l min−1, respectively. Under optimum conditions, good linear regression equations of six analytes were obtained with the detection limits ranging from 0.2 to 0.5 µg ml−1. The proposed method has been applied successfully for the quantification of GSH and PCs in Perilla frutescens (a cadmium hyperaccumulator) under cadmium stress. The recoveries were between 82.9% and 115.3%.

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Section: Calibration Curves Linear Ranges and Detection Limitsmentioning

confidence: 68%

Development of the HPLC–ELSD method for the determination of phytochelatins and glutathione inPerilla frutescensunder cadmium stress conditions

et al. 2018

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“…For more details about working electrode preparation see ref. [11]. A stainless steel was used as an auxiliary electrode.…”

Section: Instrumentationmentioning

confidence: 99%

“…In those cases the use of a more selective detection plays a crucial role. In previous works [10][11][12], VD-MCR-ALS detection mode has been applied as a first approach to quantify overlapping peaks. Despite its benefits, VD-MCR-ALS owned some shortcomings: i) when compounds with very similar electrochemical responses are analyzed, the potential range where the signals can be resolved is very narrow, and this introduces an important error in the mathematical solution, and ii) the concentrations required for a satisfactory quantization were higher than in AD.…”

Section: Introductionmentioning

confidence: 99%

“…The basis of MCR-ALS is to decompose mathematically the experimental data matrix (I) into a product of two orthogonal matrices, represented as C (containing the concentration elution profiles of resolved electroactive species at the detector) and V T (corresponding to pure voltammograms), plus an error matrix X (including the variance not explained by C and V T ) [10][11][12]:…”

Section: Introductionmentioning

confidence: 99%

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Development and Possibilities of Multichannel Voltammetric Detection in Liquid Chromatography

et al. 2010

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Voltammetric detection in HPLC by using linear potential sweeps applied to dual electrode cells is evaluated. The application of an offset between both potential sweeps, applied to either serial or parallel setup of both working electrodes, and the further subtraction of the corresponding datasets are discussed. The use of multivariate curve resolution for the treatment of overlapping signals is also investigated. Despite some noise problems, mostly derived from the differences between the working electrodes, the proposed multichannel method appears to be promising. The different approaches are tested by analyzing some thiols mixtures.

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“…[1][2][3] So the study of the L-cysteine oxidation and its detection is an important work. To date, many analytical approaches have been developed for L-cysteine detection such as fluorometry based on fluorescent dyes, [4][5][6][7] electrochemical analysis [8][9][10][11] and chromatography separation 12,13 and so on. Compared with other options, electrochemical techniques have the inherent advantages of simplicity, ease of miniaturization, high sensitivity, and relatively low cost.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Electrochemical Behaviour of Silver Pentacyanonitrosylferrate Film Modified Glassy Carbon Electrode and Its Electrocatalytic Oxidation to L‐cysteine

Liu

Dai

2011

J Chinese Chemical Soc

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A glassy carbon (GC) electrode modified with silver pentacyanonitrosylferrate (AgPCNF) film as a redox mediator was fabricated. Cyclic voltammetry was used to study the redox property of AgPCNF modified electrode. The modified electrode showed a well-defined redox couple due to [system. The effects of scan rates, supporting electrolytes and solution pHs were studied on the electrochemical behavior of the modified electrode. The feasibility of using the AgPCNF modified electrode to measure L-cysteine was investigated. It showed an excellent electrocatalytic activity towards the oxidation of L-cysteine and the anodic currents were proportional to the L-cysteine concentration in the range of 0.1 mM to 20 mM, the linear regression equation is I pa (mA) = -68.58 -5.78C L-cysteine (mM), with a correlation coefficient 0.998 for N = 23. The detection limit was down to 3.5 × 10 -8 M (three times the ratio of signal to noise).

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Comparison of voltammetric detection assisted by multivariate curve resolution with amperometric detection in liquid chromatographic analysis of cysteine-containing compounds

Cited by 22 publications

References 28 publications

Development of the HPLC–ELSD method for the determination of phytochelatins and glutathione inPerilla frutescensunder cadmium stress conditions

Development of the HPLC–ELSD method for the determination of phytochelatins and glutathione inPerilla frutescensunder cadmium stress conditions

Development and Possibilities of Multichannel Voltammetric Detection in Liquid Chromatography

Preparation and Electrochemical Behaviour of Silver Pentacyanonitrosylferrate Film Modified Glassy Carbon Electrode and Its Electrocatalytic Oxidation to L‐cysteine

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