Comparison of vitrified outcomes between human early blastocysts and expanded blastocysts

Song, Wenyan; Wang, Xue-Gai; Jin, Haixia; Yao, Guidong; Zhang, Xiangyang; Sheng, Shi; Yang, Hongyi; Peng, Zhaoliang; Sun, Yingpu

doi:10.1007/s11626-016-0009-1

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…Blastocoele collapse can be achieved mechanically by aspiring the blastocoele liquid with a micropipette or by inducing a releasing of this liquid using a laser shot 22,34 . Several studies have shown that high-quality early blastocyst embryos (G1 and G2) do not systematically develop to viable fully expanded blastocyst able to hatch and implant 29,32,35 . The advantage of using fully expanded blastocyst is associated with the fact that these embryos may have been selected further through culture and thus have a higher implantation and live birth rates than less expanded ones (G1, G2).…”

Section: Discussionmentioning

confidence: 99%

Vitrification of blastocysts at various degrees of blastocoele expansion using different exposure times to the equilibration solution

Lamin¹,

Santana²,

Senn³

et al. 2018

Hum. Reprod. Arch.

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objectives: To evaluate the efficiency of a vitrification protocol of murine blastocysts, varying the exposure time to the equilibrium solution, at different degrees of blastocoel expansion. Methods: Sixty female mice were superovulated with 10 IU of equine chorionic gonadotropin (eCG, Novormon  , Syntex, Argentina) and after 48 hours with 10 IU of human chorionic gonadotropin (hCG, Vetecor  , Calier, Barcelona, Spain). Females were then coupled with males overnight, and the presence of vaginal plug indicated that mating had occurred. Three days later, females were euthanized and the embryos at the morula stage (n = 925) were recovered by flushing the uterine tubes with GV-Hepes medium (Ingámed  , Maringá, Brazil). Embryos were then cultivated until the blastocyst stage and classified, at the time the vitrification process was initiated, into 3 groups (G1, G2, G4) according to the degree of blastocoele expansion. A control group remained in culture until hatching. All embryos, after warming, were put back into culture for measurement of the rehydration (RR) and hatching (HR) rates. Results: For G1 group, the mean RR and HR obtained over a range of exposure times to the equilibration solution of 94.0 and 84.4%, respectively. For G2, these rates were 95.2 and 87.1%, respectively. For G1 and G2, RR and HR obtained after an equilibration time of 9 min were statistically higher than those obtained after 10, 11, 12, 13 and 14 min. For G4, RR and HR were 76.4 and 69.9%, respectively, but in this case, an equilibration time of 15 min presented statistically higher survival rates compared to shorter exposition times. conclusions: The results of this study indicate that the variation in the exposure time of the embryos to the equilibration solution influences significantly the rehydration and hatching of the blastocysts, and the degree of blastocoel expansion is inversely correlated with the survival and development potential after warming.

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Section: Discussionmentioning

confidence: 99%

Vitrification of blastocysts at various degrees of blastocoele expansion using different exposure times to the equilibration solution

Lamin¹,

Santana²,

Senn³

et al. 2018

Hum. Reprod. Arch.

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“…All these AS methods have been shown to improve blastocyst survival rate, implantation rate and pregnancy rate [7, 9, 11, 12, 17, 18]. However, only a few studies focused on the comparison of these AS methods when applied prior to vitrification.…”

Section: Discussionmentioning

confidence: 99%

Effect of different artificial shrinkage methods, when applied before blastocyst vitrification, on perinatal outcomes

Wang

Feng

Zhang

et al. 2017

Reprod Biol Endocrinol

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BackgroundIn recent years, single blastocyst transfer combined with vitrification has been applied widely, which can maximize the cumulative pregnancy rate in per oocyte retrieval cycles and minimize the multiple pregnancy rate. Thus, the guarantee for these is the effectiveness of vitrified blastocyst. Studies has shown that AS of the blastocoel cavity prior to vitrification can reduce injuries, increase the thawed blastocyst survival rate and implantation rate. Several AS methods have been established. However, only a few studies have compared the effectiveness and safety of these AS methods. In this study, we aimed to compare the clinical outcomes and neonatal outcomes in FET cycles with single blastocyst that were artificially shrunk before vitrification by either LAS or MNAS method.MethodsA retrospective comparative study of FET cycles in infertile patients which were at our clinic between January 2013 and December 2014. These FET cycles were divided into two groups by the shrinking methods used before vitrification and the clinical and neonatal outcomes were assessed.ResultsThere were no statistically differences in blastocyst survival rates (95.40% vs 94.05%, P > 0.05) between the LAS and MNAS groups. However, compared with MNAS, LAS improved the warmed blastocyst implantation/clinical pregnancy rate (60.82% vs 54.37%, P < 0.05), live birth rate (50.43% vs 45.22%, P < 0.05) and also increased the monozygotic twin rate (4.07% vs 1.73%, P < 0.05). There were no differences in the average gestational weeks (38.83 ± 1.57 vs 38.74 ± 1.75), premature birth rate (0.30% vs 0.49%), average birth weight (3217.89 ± 489.98 g vs 3150.88 ± 524.03 g), low birth weight rate (5.60% vs 8.63%) and malformation rate (0.59% vs 0.48%) (P > 0.05).ConclusionsNo significant differences in neonatal outcomes were observed, while in clinical outcomes, LAS improved the warmed blastocyst implantation/clinical pregnancy rate and live birth rate markedly, there was also an increased risk of monozygotic twin pregnancies.

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Low-level laser therapy modulates demyelination in mice

Duarte

Soares

Magri

et al. 2018

Journal of Photochemistry and Photobiology B: Biology

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Comparison of vitrified outcomes between human early blastocysts and expanded blastocysts

Cited by 3 publications

References 22 publications

Vitrification of blastocysts at various degrees of blastocoele expansion using different exposure times to the equilibration solution

Vitrification of blastocysts at various degrees of blastocoele expansion using different exposure times to the equilibration solution

Effect of different artificial shrinkage methods, when applied before blastocyst vitrification, on perinatal outcomes

Low-level laser therapy modulates demyelination in mice

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