Comparison of Viral and Nonviral Vectors for Gene Transfer to Human Endothelial Progenitor Cells

Abstract

We have found adenovirus to be the most efficient of the vector systems tested for gene delivery to EPCs, an effect that is mediated almost entirely by one of two identified subpopulations.

Highly efficient lentiviral transduction of phenotypically and genotypically characterized endothelial progenitor cells from adult peripheral blood

“…Based thereon, we were able to obtain much higher transduction rates for lentiviral vectors (up to 99%) than those described by Kealy et al [17] at lower MOIs. Even with retroviral vectors, transduction rates were above 70%, that is, comparable with those seen with adenoviral vectors.…”

“…As shown by Kealy et al [17], genetic modification with adenoviral vectors did not change the functional capabilities of EPCs. Different groups have introduced Equal amounts of amplified RNA from endothelial progenitor cells (EPCs) and freshly isolated mononuclear cells (MNCs) were labeled with Cy5 and Cy3 and hybridized to a PIQOR Stem Cell microarray.…”

“…In a recently published comprehensive study, Kealy et al [17] compared different vector types with regard to their capability to transduce EPCs. In that work, adenoviral vectors turned out to be most efficient, facilitating transduction of up to 80% of a subpopulation of EPCs, though at MOIs of 500 or even 1000.…”

Novel Gene Transfer Systems: Intelligent Gene Transfer Vectors for Gene Medicines

“…In addition, adenoviral vectors have the capability of efficiently transducing endothelial progenitor cells. The efficiency of delivering the green fluorescent protein (GFP) reporter gene using an adenoviral vector was 80%, but only up to 35% using liposomes [4].…”

Comparison of DNA Delivery and Expression Using Frequently Used Delivery Methods

“…The optimal gene delivery method for a given therapy will be dependant on tissue location, and type, as well as therapeutic strategy. While certain studies have been reported comparing the efficiencies from different vectors (Wang, A. Y. et al 2004;Kealy et al 2009), given the paucity of information regards direct comparisons between various delivery techniques, especially in the cancer setting, this study assesses the level and duration of reporter gene expression within target murine tissues when delivered by a range of commonly used gene delivery techniques; electroporation, sonoporation, lipofection, Adenovirus and Adeno-Associated Virus.…”