Comparison of various transport media on human periodontal ligament cell viability

Olson, B.D.; Mailhot, Jason M.; Anderson, Ronald W.; Schuster, George S.; Weller, R. Norman

doi:10.1016/s0099-2399(97)80399-8

Cited by 46 publications

(78 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Milk is highly regulated for content sterility and production specification. It also has antibacterial, antifungal, antiviral and antiparasite properties (Olson et al, 1997;Fagade, 2004).…”

Section: Methodsmentioning

confidence: 99%

The Effect of two Different Culture Media on the Proliferation of Dental Pulp Stem Cells: Bovine Fetal Serum and Platelet Lysate

Cicero

Nanda

Martini³

2015

Current Research in Dentistry

View full text Add to dashboard Cite

Mesenchymal Stem Cells (MSCs) have been isolated from a variety of fetal and adult tissues and are considered an ideal source for cell-based therapy due to their unique properties such as multipotency and immunomodulatory functions. The aim of this work is to observe, analyze and investigate the morphology and proliferation of human dental pulp stem cells cultured in two different culture media: Fetal Bovine Serum (FBS) or platelet lysate. These cells would be cultured for a minimum period of 14 days to 3 months. The desired outcome is a pure culture of mesenchymal stem cells. With this present study we will determine which culture mediumpromotesthe most rapid formation of a pure culture of mesenchymal stem cells derived from Dental Pulp Stem Cells (DPSC).

show abstract

“…Milk is highly regulated for content sterility and production specification. It also has antibacterial, antifungal, antiviral and antiparasite properties (Olson et al, 1997;Fagade, 2004).…”

Section: Methodsmentioning

confidence: 99%

The Effect of two Different Culture Media on the Proliferation of Dental Pulp Stem Cells: Bovine Fetal Serum and Platelet Lysate

Cicero

Nanda

Martini³

2015

Current Research in Dentistry

View full text Add to dashboard Cite

show abstract

“…O meio que apresentar uma osmolalidade acima de 230 mOsm kg -1 é considerado uma [15][16][17] . Save-A-Tooth é comercializado em um kit que contém um pequeno cesto em que o dente avulsionado é suspenso e submerso em HBSS.…”

Section: Solução Salina Balanceada De Hank (Hbss)unclassified

“…Seu pH fica entre 7,4 a 7,8. O armazenamento em água de torneira pode ser tão destrutivo para o ligamento periodontal, quanto deixar o dente seco, levando a anquilose e a reabsorção por substituição 26,28,29 .…”

Section: áGua De Torneiraunclassified

Meios De Armazenamento Para Dentes Avulsionados - Uma Revisão De Literatura

Flôres¹,

Flores

Diesel

et al. 2016

Saúde (Sta. Maria)

View full text Add to dashboard Cite

RESUMOO objetivo deste estudo foi encontrar, através de uma revisão de literatura, o meio mais adequado para o armazenamento de dentes avulsionados. Esse meio deve preservar a vitalidade, aderência e a capacidade clonogênica das células do ligamento periodontal. Os meios selecionados para essa revisão de literatura foram: solução salina balanceada de Hank (HBSS), própolis, água de coco, leite, chá verde, água de torneira, clara de ovo, saliva, Gatorade, amora, ViaSpan, chá verde, água de coco, extrato de amora, própolis e clara de ovo apresentaram bons resultados nos estudos selecionados, porém necessitam mais estudos que comprovem sua eficácia. HBSS e ViaSpan tiveram os melhores resultados, apresentando grande potencial de manter as células do ligamento periodontal viáveis após a avulsão. O leite demonstrou resultados satisfatórios, boas propriedades, baixo custo e fácil disponibilidade à população, sendo este o meio ideal para armazenamento de dentes avulsionados, quando HBSS e ViaSpan forem indisponíveis. Descritores ABSTRACTThe aim of this study was to find, through a literature review, the most appropriate means for storage of avulsed teeth. Such means shall preserve the vitality, adherence and clonogenic capacity of cells of the periodontal ligament. The selected means for this literature review were: Hank's balanced salt solution (HBSS), propolis, coconut water, milk, green tea, tap water, egg white, saliva, Gatorade, blackberry, ViaSpan. green tea, coconut water, mulberry extract, propolis and egg white showed good results in selected studies, but need more studies to prove its effectiveness. HBSS and ViaSpan had the best results, presenting great potential to maintain the cells in viable periodontal ligament after avulsion. The milk was satisfactory, good properties, low cost and easy availability to the population, which is the ideal medium for storage of avulsed teeth when HBSS and ViaSpan are unavailable.

show abstract

“…3,7,11,14,18,19,24,26 • Solución balanceada de HanK's (HBSS): Es un medio de cultivo estándar usado en la investigación biomédica para la conservación celular. Tiene un pH de 7,2 y su osmolalidad es de 270 -320 mOsm/Kg 8,19 , evita la reabsorción radicular en un 91 %. En algunos países está comercializado en farmacias (Save-a-Tooth® Sat), como un pequeño contenedor con una canastilla interna que evita al mínimo el daño al ligamento periodontal durante el transporte, contiene solución de Hank's, para que el diente pueda ser introducido mientras se acude a la consulta dental para el reimplante, la desventaja es que es costoso y tiene una caducidad de dos años.…”

Section: -28unclassified

“…8 • En su composición deben tener elementos que nutran las células del ligamento periodontal que aún permanecen viables.…”

unclassified

Medios de almacenamiento y transporte para dientes avulsionados

Solar¹

2014

Odontol. sanmarquina

View full text Add to dashboard Cite

Artículo De revisiónResumen Se realizó una revisión de la literatura con respecto a los medios de almacenamiento para dientes avulsionados. Se utilizó el buscador Medline, incluyendo aquellas publicaciones entre 1980 -2007 en las que apareciesen como palabra clave: "viability", "storage medium", "storage media", "tooth avulsion" y "avulsion". Los mejores medios son aquellos que tienen las siguientes características: pH y osmolalidad fisiológica, contengan nutrientes, sean líquidos estériles y estén disponibles en el lugar del accidente. Los resultados de estos estudios muestran que la leche fresca descremada pasteurizada fue mejor que la saliva, el agua, las soluciones para lentes de contacto y el Gatorade®. El HBSS y el Viaspan fueron mejores que la leche, el propóleo y el agua de coco. El Viaspan fue igual a, o mejor que, el HBSS para preservar las células del ligamento periodontal. A pesar de su efectividad en mantener la viabilidad celular, el Viaspan y el HBSS son costosos y no están disponibles rápidamente en el lugar del accidente a diferencia de la leche. AbstractA review of the literature was made regarding the way avulsed teeth were stored. An online search in Medline was used, including those publications between 1980 and 2007 in which the key word: "viability", "storage medium", "storage media", "tooth avulsion" and "avulsion" appeared. The best storages are those that have the following characteristics: pH and physiological osmolality, contain nutrients, are liquid sterile and are available at the moment of the accident. The results of those studies showed that low-fat fresh milk pasteurized was better than saliva, water, contact lens solutions and the Gatorade®. HBSS and Viaspan were better than milk, propolis and tender Coconut water. Viaspan was equal to, or better than, HBSS in preserving periodontal ligament cells. Unfortunately, despite of its effectiveness in maintaining periodontal ligament cells viability, Viaspan and HBSS are expensive and are not available like milk. Odontología Sanmarquina IntroduccionLos traumatismos dentarios en niños son el segundo motivo de consulta después de la Caries Dental y son un problema de salud pública, no porque su prevalencia sea alta, sino por las secuelas que ocasiona. 1 El tratamiento de la avulsión es el reimplante, pero el porcentaje de éxito a largo plazo varía entre el 4-70%, ya que va a depender de las condiciones clínicas específicas de cada caso en particular.2, 3 Para el pronóstico los factores críticos son:• El período extraoral (Tiempo que el diente está fuera de boca = Tiempo en medio seco + tiempo en medio húmedo).• El medio de almacenamiento (porque los fibroblastos al no e s t a r i r r i g a d o s s e d e s e c a n rápidamente).El tiempo en medio seco es el factor clínico más asociado con el desarrollo de la anquilosis post-reimplante o reabsorción de reemplazo.2,3,4,5 ObjetivosRealizar una revisión de la literatura publicada usando el enfoque basado en evidencia para actualizar la información con respecto a los medios de almacenamiento ...

show abstract

Comparison of various transport media on human periodontal ligament cell viability

Cited by 46 publications

References 14 publications

The Effect of two Different Culture Media on the Proliferation of Dental Pulp Stem Cells: Bovine Fetal Serum and Platelet Lysate

The Effect of two Different Culture Media on the Proliferation of Dental Pulp Stem Cells: Bovine Fetal Serum and Platelet Lysate

Meios De Armazenamento Para Dentes Avulsionados - Uma Revisão De Literatura

Medios de almacenamiento y transporte para dientes avulsionados

Contact Info

Product

Resources

About