Comparison of urinary and recombinant follicle-stimulating hormone in in vitro growth, maturation, and fertilization of mouse preantral follicles

Calongos, Giannina; Hasegawa, Akiko; Komori, Shinji; Koyama, Koji

doi:10.1016/j.fertnstert.2007.04.058

Cited by 13 publications

(13 citation statements)

References 37 publications

(54 reference statements)

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“…In recent studies, α-minimal essential medium and Dulbecco's modified Eagle's medium were used as basal media for the in vitro growth of preantral follicles [35,42,48,[53][54][55]. Both media were supplemented with various culture additives, including serum, growth factors, gonadotropins, antibiotics and substrates.…”

Section: Protocol For In Vitro Growth Of Preantral Folliclesmentioning

confidence: 99%

Derivation of Histocompatible Stem Cells from Ovarian Tissue

2010

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Abstract. Somatic cell nuclear transfer, the first established technique for producing patient-specific autologous stem cells, inevitably requires the sacrifice of viable embryos. To circumvent the serious ethical issues associated with this use of embryos, researchers have developed several alternative methods for the production of histocompatible stem cells. In our research, we have used two methods to derive histocompatible stem cells from murine ovarian tissue. First, we have established autologous stem cells by culturing degeneration-fated preantral follicles to produce developmentally competent, mature oocytes and then parthenogenetically activating these mature oocytes to acquire genetic homogeneity. Second, we have used cell-to-cell interactions to derive stem cells from ovarian stromal cells without undertaking genetic modification. We have successfully derived autologous murine stem cells by manipulating primary and early secondary follicles in vitro, and this method has proved successful even for follicles retrieved from aged ovaries. Furthermore, we believe that it will be possible to isolate stem cells directly from nongermline ovarian tissue or to derive stem cells by culturing the ovarian cells with other somatic cells. If achieved, these aims will greatly advance the development of induced pluripotent stem cell technology, as well as tissue-specific stem cell research. In this review, we introduce the relevant technologies for establishing histocompatible stem cells from ovarian tissue cells without undertaking genetic manipulation and review the current limitations of, and future research directions in, stem cell biology. Key words: Autologous, Ovarian cells, Parthenogenesis, Preantral follicle, Stem cell (J. Reprod. Dev. 56: [481][482][483][484][485][486][487][488][489][490][491][492][493][494] 2010) luripotent stem cells are widely recognized as critical to the success of certain cutting-edge medical technologies, such as cell replacement, organ regeneration, tissue engineering, and rejuvenation therapy [1][2][3]. However, the potential clinical practicability of stem-cell-based techniques is limited by the absolute requirement for patient-stem cell histocompatibility or patient acquisition of immune tolerance [4]. Another obstacle to the use of stem cells is the current lack of effective techniques for regulating cell-to-cell interactions and for inducing target differentiation of stem cells. The future success of tissue regeneration therapies will also depend on the optimization of stem-cell transplantation techniques and the development of strategies for inducing transplanted stem cells to resume their normal functions. In addition, all stem cell-based procedures must be carried out in an ethically acceptable manner.Efforts to develop stem-cell-based techniques such as those described above have been extensive. In our research, we have consistently attempted to develop novel methodologies for the establishment of histocompatible stem cells. At a very early stage in stem cell research, a ...

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Section: Protocol For In Vitro Growth Of Preantral Folliclesmentioning

confidence: 99%

Derivation of Histocompatible Stem Cells from Ovarian Tissue

2010

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“…Only few studies have reported hormone assays in medium collected during follicle culture. Subsequently, secretion of hormones, essentially estradiol, testosterone and progesterone, produced in vitro by mice follicles have been used as a test of functionality (Demeestere et al, 2002;Liu et al, 2002;Wycherley et al, 2004;Calongos et al, 2008). The same report demonstrated the secretion of estradiol and progesterone during follicle culture.…”

Section: Introductionmentioning

confidence: 98%

Secretion profiles from in vitro cultured follicles, isolated from fresh prepubertal and adult mouse ovaries or frozen–thawed prepubertal mouse ovaries

Dorphin

Prades-Borio

Anastácio

et al. 2011

Zygote

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In vitro folliculogenesis could be a new technology to produce mature oocytes from immature follicles that have been isolated from cryopreserved or fresh ovarian tissue. This technique could also be a tool for evaluation of oocyte quality and/or for determination of follicular parameters during follicular growth. Our objective was to characterize in mice the secretion profiles of follicles that had been isolated mechanically during in vitro follicular growth and in relation to the growth curve. Early preantral follicles from fresh prepubertal and adult mouse ovaries or frozen-thawed prepubertal mouse ovaries were cultured individually in microdrops under oil for 12 days. Each day, two perpendicular diameters of the follicles were measured. From day-3 to day-12 of culture, culture medium was collected and preserved for determination of inhibin B, anti-Müllerian hormone (AMH) and estradiol levels. At the end of the culture, after maturation, the status of the oocyte was evaluated. Follicular growth and their individual hormone production did not always correlate. Inhibin B was never secreted from follicles of less than 200 μm diameter, whether the follicles were examined when fresh or after freezing-thawing. Estradiol secretion was never observed in frozen-thawed follicles. AMH was mainly secreted between day-3 and day-9. Despite similar morphological aspects at the start of culture, follicles selected for in vitro folliculogenesis were found to be heterogeneous and differed in their ability to grow and to produce hormones, even if they had similar growth curves. Follicles from frozen-thawed ovaries developed slowly and produced fewer hormones than freshly collected follicles.

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“…As these follicles account for the vast majority of follicles (95%) in the ovary [1], they can provide a great number of potentially viable oocytes for in vitro reproductive techniques, such as in vitro fertilization (IVF) and cloning. Technologies being developed for isolation [2, 3], cryopreservation [4], and culture [5] of primordial follicles strive to prevent follicular atresia by optimizing the culture of these follicles in vitro.…”

Section: Introductionmentioning

confidence: 99%

Ultrastructure of Sheep Primordial Follicles Cultured in the Presence of Indol Acetic Acid, EGF, and FSH

Andrade

Maddox-Hyttel

Landim-Alvarenga

et al. 2011

Veterinary Medicine International

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The aim of this study was to investigate the ultrastructural characteristics of primordial follicles after culturing of sheep ovarian cortical slices in the presence of indol acetic acid (IAA), Epidermal Growth Factor (EGF), and FSH. To evaluate ultrastructure of primordial follicles cultured in MEM (control) or in MEM containing IAA, EGF, and FSH, fragments of cultured tissue were processes for transmission electron microscopy. Except in the control, primordial follicles cultured in supplemented media for 6 d were ultrastructurally normal. They had oocyte with intact nucleus and the cytoplasm contained heterogeneous-sized lipid droplets and numerous round or elongated mitochondria with intact parallel cristae were observed. Rough endoplasmic reticulum (RER) was rarely found. The granulosa cells cytoplasm contained a great number of mitochondria and abundant RER. In conclusion, the presence of IAA, EGF, and FSH helped to maintain ultrastructural integrity of sheep primordial follicles cultured in vitro.

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Comparison of urinary and recombinant follicle-stimulating hormone in in vitro growth, maturation, and fertilization of mouse preantral follicles

Cited by 13 publications

References 37 publications

Derivation of Histocompatible Stem Cells from Ovarian Tissue

Derivation of Histocompatible Stem Cells from Ovarian Tissue

Secretion profiles from in vitro cultured follicles, isolated from fresh prepubertal and adult mouse ovaries or frozen–thawed prepubertal mouse ovaries

Ultrastructure of Sheep Primordial Follicles Cultured in the Presence of Indol Acetic Acid, EGF, and FSH

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