Comparison of ultrasonography, bovine pregnancy-specific protein B, and bovine pregnancy-associated glycoprotein 1 tests for pregnancy detection in dairy cows

Szenci, O.; Beckers, Jean‐François; Humblot, P.; Sulon, Joseph; Sasser, G.; Taverne, M.A.M.; Várga, J.; Baltusen, R.; Schekk, Gy.

doi:10.1016/s0093-691x(98)00115-0

Cited by 119 publications

(100 citation statements)

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“…The presence of PAGs can be measured by radioimmunologic (RIA) and immunoenzymatic (ELISA) tests. These PAG tests allow for early pregnancy diagnoses in domestic cattle [69,80,91,[95][96][97][98], sheep [70,[99][100][101][102][103][104] and goats [105,106]. Moreover, heterologous PAG tests were also useful for pregnancy diagnoses in wild ruminants, i.e.…”

Section: Pregnancy Diagnostic Pag Testsmentioning

confidence: 99%

“…wood bison [107], moose [108], rocky mountain elk [109], white-tailed deer [110,111], mule deer [111], red deer [112], sika deer [113], fallow deer [114,115], mountain goat [116] and Alpine goats [117][118][119]. The measurement of PAG protein concentrations in maternal blood or milk of ruminants allow for 76.6-100% accuracy rates for early pregnancy diagnoses [120,121] and provide an alternative method to ultrasonography for determining late embryonic mortality [96,122].…”

Section: Pregnancy Diagnostic Pag Testsmentioning

confidence: 99%

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Biodiversity of multiple Pregnancy-Associated Glycoprotein (PAG) family: gene cloning and chorionic protein purification in domestic and wild eutherians (Placentalia) – a review

2006

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-This review presents a broad overview of chorionic glycoproteins encoded by the Pregnancy-Associated Glycoprotein (PAG) gene family and also serves to illustrate how the recent discovery of the PAG family has contributed to our general knowledge of genome evolution, placental transcription and placental protein expression. The complex and large PAG family is restricted to the Artiodactyla order, although single PAG-like genes have also been identified in species outside the Artiodactyla. The PAGs are members of the aspartic proteinase (AP) superfamily. Unexpectedly, however, some members of the PAG family possess amino acid substitutions within and around the active site that likely render them unable to act as proteinases. This paper summarises the available information regarding biodiversity of PAG gene expression based on cDNA cloning, mRNA localisation studies and the structural organisation of the PAG genes with a particular emphasis on PAG promoters. It also compares available data regarding PAG protein purifications, sequencing and their N-glycodiversity. Finally, it discusses the scientific relevance, possible functional roles of the PAGs and describes possible profitable applications related to the detection of PAG proteins in the blood of pregnant domestic and wild species. chorionic glycoproteins / eutherian PAG family / diagnostic PAG markers

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Section: Pregnancy Diagnostic Pag Testsmentioning

confidence: 99%

Section: Pregnancy Diagnostic Pag Testsmentioning

confidence: 99%

Biodiversity of multiple Pregnancy-Associated Glycoprotein (PAG) family: gene cloning and chorionic protein purification in domestic and wild eutherians (Placentalia) – a review

2006

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“…Several studies on lactating dairy cows have indicated that LEM between 27 and 45 d after AI range from 8 to 21% [33][34][35]. In some cases, LEM have been reported to be higher than 21% [21,36].…”

Section: Ma Alnimermentioning

confidence: 99%

Comparison of an oestrus synchronisation protocol with oestradiol benzoate and PGF2α and insemination at detected oestrus to a timed insemination protocol (Ovsynch) on reproductive performance of lactating dairy cows

Alnimer

2005

Reprod. Nutr. Dev.

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-A total of 226 out of 245 postpartum lactating dairy cows in a commercial dairy farm were allocated to two groups of oestrous synchronisation protocols in order to evaluate reproductive performance. One group was treated with oestradiol benzoate (ODB) and PGF 2α on day 10 of the oestrous cycle with insemination at the detected oestrus, the second group underwent the Ovsynch (OVS) protocol (GnRH + PGF 2α + GnRH) with timed AI. Pregnancy was diagnosed by ultrasonography on day 28 after AI and confirmed by rectal palpation on day 45. A higher (P < 0.001) proportion of cows in OVS (100%) were inseminated within (19.2 ± 3.8 h) following the second GnRH injection than those of cows in EPE (ODB + PGF 2α + ODB) (70.6%) inseminated at the detected oestrus within (35.6 ± 5.2 h) following the second ODB injection. Pregnancy rates for the first AI at day 28 (64.0 ± 4.6, 62.4 ± 5.5%) and at day 45 post-insemination (40.4 ± 4.7, 40.0 ± 5.6%) for OVS and EPE cows respectively, did not differ between the two treatments, whereas, the overall pregnancy rates tended to be higher (P < 0.08) for the OVS (85.1 ± 3.8%) cows than the EPE cows (74.1 ± 4.5%). No differences were observed in pregnancy rates for first AI and overall up to fourth AI between primiparous (34.7 ± 5.8 and 85.3 ± 4.7%) and multiparous cows (43.5 ± 4.5 and 77.4 ± 3.6%). Days open for pregnant cows tended to be lower (P < 0.08) for OVS (76.2 ± 3) than for EPE cows (84.7 ± 4), while days open were higher (P < 0.05) in primiparous cows (85.3 ± 4) than in multiparous cows (75.6 ± 3). The results indicate that pregnancy rates for first AI were similar, but overall pregnancy rates up to the fourth AI tended to be higher for OVS than EPE cows, while days open was tended to be lower for OVS than EPE cows. dairy cows / oestrus-synchronisation / GnRH / oestradiol / PG / reproductive performance

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“…The amounts of BC products in maternal circulation appear to be associated with placental mass, foetal number and neonatal birth weight, and have been postulated to be an index for conceptus viability and pregnancy normalcy in cattle (Byatt et al 1992a, Patel et al 1995, Vasquez et al 1995, Szenci et al 1998, Tefera et al 2001, Ravelich et al 2004. Associations between abnormal placental and foetal development after in vitro embryo manipulations have been suggested to play a key role in the occurrence of high birth weights.…”

Section: Introductionmentioning

confidence: 99%

“…Despite the unknown function and control of PSPB secretion, many potential biological functions have been suggested based on in vivo and in vitro effects, including prostaglandin release by the endometrium, implantation, immunotolerance of the conceptus as a tissue alograft and uterine remodelling after parturition, among others (Del Vecchio et al 1990, Kiracofe et al 1993, Austin et al 1999, Tefera et al 2001. Both PAG-1 and PSPB diagnostic assays have been developed for early plasma diagnosis of pregnancy in cattle and other ruminants (Sasser et al 1986, Zoli et al 1991, Green et al 2000.The amounts of BC products in maternal circulation appear to be associated with placental mass, foetal number and neonatal birth weight, and have been postulated to be an index for conceptus viability and pregnancy normalcy in cattle (Byatt et al 1992a, Patel et al 1995, Vasquez et al 1995, Szenci et al 1998, Tefera et al 2001, Ravelich et al 2004. Associations between abnormal placental and foetal development after in vitro embryo manipulations have been suggested to play a key role in the occurrence of high birth weights.…”

mentioning

confidence: 99%

Expression profile and protein levels of placental products as indirect measures of placental function in in vitro-derived bovine pregnancies

Bertolini¹,

Wallace²,

Anderson³

2006

Reproduction

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Bovine conceptus development and its association with placental proteins present in maternal, foetal and neonatal plasma and foetal (amniotic and allantoic) fluids were investigated in in vivo-and in vitro-produced (IVP) concepti and newborn calves. Females were superovulated to obtain control embryos, whereas IVP embryos were derived from established in vitro procedures. Pregnant animals were slaughtered on days 90 or 180 of gestation or allowed to develop to term for the assessment of physical traits. Foetal, maternal and neonatal blood and foetal fluids were collected for the determination of bovine placental lactogen (bPL) and bovine pregnancy-specific protein B (bPSPB) concentrations. Placental transcripts for bPL and bPSPB, determined by quantitative RT-PCR, were elevated in IVP placentomes. No major physical differences were observed between groups on day 90, but concentrations of bPL and bPSPB were higher in foetal plasma and allantoic fluid of IVP concepti in day 180 pregnancies, which were correlated with larger uterine and conceptus traits. Maternal concentrations of bPL in IVP pregnancies were lower than controls during the last 8 weeks of gestation, to become similar as parturition approached. Newborn IVP calves and foetal membranes were larger and displayed higher concentrations of plasma bPL than controls (10 and 60 min after birth). Our results indicated that differential patterns of secretion of bPL and bPSPB into the maternal and foetal systems occurred at distinct stages of gestation, and these were associated with altered conceptus development after in vitro embryo manipulations, indirectly demonstrating deviations in placental function in IVP pregnancies. Reproduction (2006) 131 163-173 IntroductionWith the onset of the trophoblast apposition to the endometrium during the process of bovine placenta formation and development, large granulated cells, the trophoblast binucleate cells (BCs), migrate from the trophectoderm to fuse with uterine epithelial cells (Wooding 1992), forming short-lived trinucleate cells. This phenomenon occurs throughout pregnancy in cattle, but the number and period of existence of trinucleate cells decline towards the end of gestation (Wooding 1992, Green et al. 2000. Binucleate cells comprise about 20% of the trophoblast cells and are responsible for hormones and bioactive products important for the regulation of foetal growth and development (Anthony et al. 1995, Green et al. 2000, Schlafer et al. 2000. The contents of the membranebounded secretory granules of BCs are released by exocytosis towards the maternal capillary bed, reaching the maternal blood system (Wooding 1992). Many proteins are produced by the BCs, including placental lactogen (PL) and a range of glycoproteins (Wooding 1992), such as pregnancy-specific protein B (PSPB) (Butler et al. 1982).PL lactogen is a member of the growth hormone (GH)/prolactin (PRL) gene family synthesized and stored in secretory granules of the trophoblast BCs (Byatt et al. 1992a, Wooding 1992, Gootwine 2004. Although ...

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Comparison of ultrasonography, bovine pregnancy-specific protein B, and bovine pregnancy-associated glycoprotein 1 tests for pregnancy detection in dairy cows

Cited by 119 publications

References 14 publications

Biodiversity of multiple Pregnancy-Associated Glycoprotein (PAG) family: gene cloning and chorionic protein purification in domestic and wild eutherians (Placentalia) – a review

Biodiversity of multiple Pregnancy-Associated Glycoprotein (PAG) family: gene cloning and chorionic protein purification in domestic and wild eutherians (Placentalia) – a review

Comparison of an oestrus synchronisation protocol with oestradiol benzoate and PGF2α and insemination at detected oestrus to a timed insemination protocol (Ovsynch) on reproductive performance of lactating dairy cows

Expression profile and protein levels of placental products as indirect measures of placental function in in vitro-derived bovine pregnancies

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