Comparison of two techniques for diagnosis of giardiasis in dogs

Decock, Christian; Cadiergues, Marie‐Christine; Larcher, Marie; Vermot, Solange; Franc, Michel

doi:10.1051/parasite/2003101p69

Cited by 26 publications

(28 citation statements)

References 4 publications

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“…In this study, DNA was amplified by PCR for one of the parasites even if the preceding parasitological tests were negative for that parasite. Other researchers have concluded that discordance among testing modalities is common (Decock, Cadiergues, Larcher, Vermot, & Franc, ; Dryden, Payne, & Smith, ; Geurden, Berkvens, Casaert, Vercruysse, & Claerebout, ; Leonhard, Pfister, Beelitz, Wielinga, & Thompson, ; McDowall et al, ; Olson et al, ), and that identifying and genotyping G. intestinalis can be challenging (Lebbad et al, ; Leonhard et al, ; McDowall et al, ), all of which may indicate that our prevalence estimates are likely underestimates. Our study highlights some of the realistic challenges that exist with collecting faecal samples in a remote Arctic locale, maintaining correct sample storage conditions and facilitating rapid analysis at a centralized southern laboratory.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and genetic characterization of Giardia spp. and Cryptosporidium spp. in dogs in Iqaluit, Nunavut, Canada

Julien

Sargeant

Guy

et al. 2019

Zoonoses and Public Health

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There are few epidemiologic studies on the role of dogs in zoonotic parasitic transmission in the Circumpolar North. The objectives of this study were to: (a) estimate the faecal prevalence of Giardia spp. and Cryptosporidium spp. in dogs; (b) investigate potential associations between the type of dog population and the faecal presence of Giardia spp. and Cryptosporidium spp.; and (c) describe the molecular characteristics of Giardia spp. and Cryptosporidium spp. in dogs in Iqaluit, Nunavut. We conducted two cross‐sectional studies in July and September 2016. In July, the team collected daily faecal samples for 3 days from each of 20 sled dogs. In September, the team collected three faecal samples from each of 59 sled dogs, 111 samples from shelter dogs and 104 from community dogs. We analysed faecal samples for the presence of Giardia spp. and Cryptosporidium spp. using rapid immunoassay and flotation techniques. Polymerase chain reaction (PCR) and sequencing of target genes were performed on positive faecal samples. Overall, the faecal prevalence of at least one of the target parasites, when one faecal sample was chosen at random for all dogs, was 8.16% (CI: 5.52–11.92), and for Giardia spp. and Cryptosporidium spp., prevalence was 4.42% (CI: 2.58–7.49) and 6.12% (CI: 3.88–9.53), respectively. The odds of faecal Giardia spp. in sled dogs were significantly higher than those in shelter and community dogs (OR 10.19 [CI: 1.16–89.35]). Sequence analysis revealed that 6 faecal samples were Giardia intestinalis, zoonotic assemblage B (n = 2) and species‐specific assemblages D (n = 3) and E (n = 1), and five faecal samples were Cryptosporidium canis. Giardia intestinalis is zoonotic; however, Cryptosporidium canis is rare in humans and, when present, usually occurs in immunosuppressed individuals. Dogs may be a potential source of zoonotic Giardia intestinalis assemblage B infections in residents in Iqaluit, Nunavut, Canada; however, the direction of transmission is unclear.

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Section: Discussionmentioning

confidence: 99%

Prevalence and genetic characterization of Giardia spp. and Cryptosporidium spp. in dogs in Iqaluit, Nunavut, Canada

Julien

Sargeant

Guy

et al. 2019

Zoonoses and Public Health

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“…It demonstrates the need for the use, when possible, of a larger sample size, more precise and available molecular diagnostic tools (Gasser, 2006) and repeated sampling of the same individuals. Indeed, the probability of detecting Giardia by coprological analysis in infected individuals is greatly increased by sampling over several consecutive days (Leib et al., 1992; Decock et al., 2003). Bailenger’s method was used with the aim of improving sensitivity but it had the opposite effect.…”

Section: Discussionmentioning

confidence: 99%

Epidemiological Study of Non‐systemic Parasitism in Dogs in Southeast Mediterranean Spain Assessed by Coprological and Post‐mortem Examination

Martínez‐Carrasco

Berriatua

Garijo-Toledo

et al. 2007

Zoonoses and Public Health

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The prevalence and risk factors of non-systemic canine ecto- and endoparasitism and anthelminthic use in Murcia located at the centre of the Spanish Mediterranean coastal arch, was investigated by coprology and necropsy in up to 275 pet, city shelter and stray dogs in 2001-2004. Faecal parasite stages were detected in 25% of dogs. Species frequency was 6-10% for Toxocara canis, Ancylostomatidae spp., Toxascaris leonina and Isospora canis, and 0.4-1% for Trichuris vulpis, Giardia lamblia, and Dipylidium caninum. Logistic regression indicated that the risk of intestinal parasitism was highest for dogs

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“…Copro-antigens of G. intestinalis and C. parvum were quantified on 100 mg of feces using respectively the ProSpecT-Giardia and the ProSpecT-Cryptosporidium Microplate Assay kit (Remel, France) (Decock et al, 2003;Mekaru et al, 2007;Rimhanen-Finne et al, 2007). An optical density value > 0.05 was considered positive according to the manufacturer's instructions.…”

Section: Intestinal Parasitesmentioning

confidence: 99%

Risk factors of weaning diarrhea in puppies housed in breeding kennels

Grellet

Chastant-Maillard

Robin

et al. 2014

Preventive Veterinary Medicine

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Diarrhea represents one of the most frequent disorders in dogs. In puppies, degradation of feces quality is associated with a reduced daily weight gain and an increased risk of death. Prevention of diarrhea in puppies requires a global approach encompassing enteropathogens, environment and management practices especially when housed in groups. The purpose of this study was to determine prevalence of enteropathogens in puppies in breeding kennels and to identify risk factors of diarrhea. Two hundred and sixty six puppies (between 5 and 14 weeks of age) from 29 French breeding kennels were included. For each kennel, data about environment, management of the kennel and puppies' characteristics (age, sex and breed) were collected. For each puppy, fecal consistency and fecal excretion of enteropathogens (viruses and parasites) was evaluated. At least one enteropathogen was identified in 77.1% of puppies and 24.8% of puppies presented abnormal feces. The main risk factor of weaning diarrhea was fecal excretion of canine parvovirus type 2 (odds ratio=5; confidence interval 95%: 1.7-14.7). A targeted sanitary and medical prophylaxis against canine parvovirus type 2 should be implemented to decrease risk of weaning diarrhea.

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Comparison of two techniques for diagnosis of giardiasis in dogs

Cited by 26 publications

References 4 publications

Prevalence and genetic characterization of Giardia spp. and Cryptosporidium spp. in dogs in Iqaluit, Nunavut, Canada

Prevalence and genetic characterization of Giardia spp. and Cryptosporidium spp. in dogs in Iqaluit, Nunavut, Canada

Epidemiological Study of Non‐systemic Parasitism in Dogs in Southeast Mediterranean Spain Assessed by Coprological and Post‐mortem Examination

Risk factors of weaning diarrhea in puppies housed in breeding kennels

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