Comparison of Two Rapid Diagnostic Assays for Detection of Immunoglobulin M Antibodies to Dengue Virus

Wu, Shuenn-Jue; Paxton, Helene; Hanson, Barbara A.; Kung, Cheryl G.; Chen, Timothy B.; Rossi, Cindy A.; Vaughn, David W.; Murphy, Gerald S.; Hayes, Curtis G.

doi:10.1128/cdli.7.1.106-110.2000

Cited by 35 publications

(18 citation statements)

References 18 publications

(16 reference statements)

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“…37,[40][41][42][43] Serum, blood on filter paper, 7,44,45 and more recently saliva are useful for IgM detection if samples are taken within the appropriate time frame (after five days of onset of fever). Different commercial kits [46][47][48][49][50][51][52][53] for anti-dengue IgM and IgG detection are available, with variable figures of sensitivity and specificity (see Table 1). …”

Section: Serological Diagnosismentioning

confidence: 99%

Dengue diagnosis, advances and challenges

Guzmán

Kourı́

2004

International Journal of Infectious Diseases

276

225

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Dengue diagnosis was one of the topics discussed at the symposium 'The Global Threat of Dengue - Desperately Seeking Solutions' organized during the 10th International Congress of Infectious Diseases held in Singapore in 2002. In this paper, a review is presented focusing on the main advances, problems and challenges of dengue diagnosis.IgM capture ELISA, virus isolation in mosquito cell lines and live mosquitoes, dengue specific monoclonal antibodies and PCR have all represented major advances in dengue diagnosis. However, an appropriate rapid, early and accessible diagnostic method useful both for epidemiological surveillance and clinical diagnosis is still needed. Also, tools that suggest a prognosis allowing for better management are also needed. Finally, laboratory infrastructure, technical expertise and research capacity must be improved in endemic countries in order to positively influence dengue surveillance, clinical case management and the development of new approaches to dengue control.

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Section: Serological Diagnosismentioning

confidence: 99%

Dengue diagnosis, advances and challenges

Guzmán

Kourı́

2004

International Journal of Infectious Diseases

276

225

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“…This result was considered a false-positive reaction by the Panbio Dengue IgM assay, resulting in a false positivity figure of 0.5% (1/219). This is considered excellent, since higher false positive reaction rates (3 and 6%) have been reported using different ELISA formats (Lam et al, 2000;Wu et al, 2000).…”

Section: Discussionmentioning

confidence: 93%

Evaluation of immunoglobulin M and G capture enzyme-linked immunosorbent assay Panbio kits for diagnostic dengue infections

Vázquez

Hafner²,

Ruiz

et al. 2007

Journal of Clinical Virology

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“…These rapid tests, which exhibit colored lines typically due to light scattering off surfacefunctionalized metallic nano-particles, have demonstrated potential for tackling public health problems, especially in low-resource settings [36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53][54][55] .…”

Section: Introductionmentioning

confidence: 99%

Google glass based immunochromatographic diagnostic test analysis

et al. 2015

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Integration of optical imagers and sensors into recently emerging wearable computational devices allows for simpler and more intuitive methods of integrating biomedical imaging and medical diagnostics tasks into existing infrastructures. Here we demonstrate the ability of one such device, the Google Glass, to perform qualitative and quantitative analysis of immunochromatographic rapid diagnostic tests (RDTs) using a voice-commandable hands-free software-only interface, as an alternative to larger and more bulky desktop or handheld units. Using the built-in camera of Glass to image one or more RDTs (labeled with Quick Response (QR) codes), our Glass software application uploads the captured image and related information (e.g., user name, GPS, etc.) to our servers for remote analysis and storage. After digital analysis of the RDT images, the results are transmitted back to the originating Glass device, and made available through a website in geospatial and tabular representations. We tested this system on qualitative human immunodeficiency virus (HIV) and quantitative prostate-specific antigen (PSA) RDTs. For qualitative HIV tests, we demonstrate successful detection and labeling (i.e., yes/no decisions) for up to 6-fold dilution of HIV samples. For quantitative measurements, we activated and imaged PSA concentrations ranging from 0 to 200 ng/mL and generated calibration curves relating the RDT line intensity values to PSA concentration. By providing automated digitization of both qualitative and quantitative test results, this wearable colorimetric diagnostic test reader platform on Google Glass can reduce operator errors caused by poor training, provide real-time spatiotemporal mapping of test results, and assist with remote monitoring of various biomedical conditions.

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Comparison of Two Rapid Diagnostic Assays for Detection of Immunoglobulin M Antibodies to Dengue Virus

Cited by 35 publications

References 18 publications

Dengue diagnosis, advances and challenges

Dengue diagnosis, advances and challenges

Evaluation of immunoglobulin M and G capture enzyme-linked immunosorbent assay Panbio kits for diagnostic dengue infections

Google glass based immunochromatographic diagnostic test analysis

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