Comparison of Two Protocols of Carbon Tetrachloride-Induced Cirrhosis in Rats – Improving Yield and Reproducibility

Fortea, José Ignacio; Fernández-Mena, Carolina; Puerto, Marta; Ripoll, Cristina; Almagro, Jorge; Bañares, Juan; Bellón, José M.; Bañares, Rafael

doi:10.1038/s41598-018-27427-9

Cited by 55 publications

(49 citation statements)

References 19 publications

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“…Furthermore, hepatotoxicity of CCL4 model in the present work was accompanied with inflammation and fibrosis. Fibrogenesis is known to be associated with the necrosis and inflammation of the liver (Fortea et al, 2018;Wahid et al, 2018). CCL4 is considered one of the most used hepatotoxins in the experimental study of liver diseases.…”

Section: Discussionmentioning

confidence: 99%

Dandelion prevents liver fibrosis, inflammatory response, and oxidative stress in rats

Hamza

Mohamed

Lashin

et al. 2020

JoBAZ

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Background: Liver fibrosis is the main contributor to the chronic liver-associated morbidity and mortality. Purpose: The study was conducted to evaluate the effects of whole plant powder of dandelion (Taraxacum officinale) on liver fibrosis. Methods: Liver fibrosis was induced by the oral administration of 20% carbon tetrachloride (CCL4), twice a week for 8 weeks. Simultaneously, dandelion root extract (500 mg/kg) was daily administered via the same route. Results: Dandelion remarkably improved the liver histology as evidenced by histopathological scoring with hematoxylin-eosin staining. Masson staining and hydroxyproline content similarly showed that dandelion decreased collagen deposition. Both mRNA and protein levels of α-smooth muscle actin and collagens 1 and 3 have been decreased after dandelion treatment compared to CCL4 group. Dandelion also downregulated the mRNA expressions of inflammatory factors interleukin-IL-1β, tumor necrosis factor-α, remodeling growth factor-β1, cyclooxygenase-2, and nuclear factor kappa-B and decreased the myeloperoxidase activity. Additionally, the effects of dandelion were associated with the decreased levels of the hepatic oxidative stress markers (malondialdehyde and P. carbonyl) and elevation of the activity of superoxide dismutase activity. Dandelion's effect to alleviate the fibrosis and inflammation induced by CCL4 treatment in the livers and was more pronounced than with silymarin. The total antioxidant study of dandelion extract revealed that dandelion has notable ferric reducing antioxidant power and high total phenolic content. Conclusion: Finally, these results suggest that dandelion prevents the progression of hepatic fibrosis induced by CCL4. The dandelion's antifibrotic effects could be attributed to its ability to scavenge free radicals and to attenuate inflammatory cells activations.

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Section: Discussionmentioning

confidence: 99%

Dandelion prevents liver fibrosis, inflammatory response, and oxidative stress in rats

Hamza

Mohamed

Lashin

et al. 2020

JoBAZ

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“…IDWE Ixeris denticulate water extract immune, common bile duct obstruction and multi-factor combination method are the commonly used methods for preparing animal models of liver cirrhosis [24,25]. Among them, CCl 4 as a classical hepatotoxic substance is widely used in the preparation of liver cirrhosis model, which commonly used 40-60% CCl 4 -mixed oil solution twice a week for about 10 weeks [26]. In our study, 40% CCl 4 oil solution was used for establishment of the liver cirrhosis model through repeated intraperitoneal injection in small dosage.…”

Section: Discussionmentioning

confidence: 99%

Hepatoprotective effects and mechanisms of Ixeris denticulate water extract on liver cirrhosis in experimental rat

Zhu

Liu

Chen

et al. 2020

BMC Complement Med Ther

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Background: To explore the protective effect and mechanisms of Ixeris denticulate water extract (IDWE) in the development of liver cirrhosis in experimental rat. Methods: Sixty rats were randomly divided into five groups: control group, model group and IDWE (2, 4 and 8 g/ kg) treatment groups. Alanine transferase (ALT), aspartate transaminase (AST), albumin (ALB), tumor necrosis factoralpha (TNF-α), Interleukin (IL)-6 and IL-8 in serum and superoxide dismutase (SOD), malondialdehyde (MDA) in liver tissue were evaluated, respectively. The liver index, liver morphology and liver histopathological analysis were detected as a supportive data. The liver protein expression of Bcl-2 and Bax were assessed by western blot, and NF-κB p65 protein expression was determined by immunohistochemistry analysis. Results: The result showed that a significantly decrease in the levels of serum AST, ALT and serum inflammatory factors TNF-α, IL-6 and IL-8 in IDWE-treated rats. The levels of serum ALB and SOD in liver tissue were markedly increased after IDWE treated, compared with model rats. Furthermore, IDWE-treated group also exhibited a downregulated protein expression of NF-κB p65 and Bax, up-regulated Bcl-2 protein expression. Conclusions: IDWE could effectively alleviate the course of liver cirrhosis in rat model, which may be a potent hepatoprotective agent in clinical therapy in the future.

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“…However, liver index showed no difference among three CCl 4 -treated groups. Although many studies demonstrated that administration of CCl 4 markedly impacted on the body weight and liver-to-body weight ratio, the different dosage of CCl 4 , age and body weight of the rats, the duration of treatment made the diversity of consequences (Fortea et al, 2018;Umezu, S. Yuasa, & Sudoh, 1985). In addition, previous studies have shown that CCl 4 administration leads to hepatic lipid accumulation and hepatomegaly (Lu, Weng, Chen, & Sheen, 2017).…”

Section: Discussionmentioning

confidence: 99%

Camellia Oleifera Seed Extract Mildly Ameliorates Carbon Tetrachloride‐Induced Hepatotoxicity in Rats by Suppressing Inflammation

Yeh

Huang

et al. 2019

Journal of Food Science

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The objective of this study was to evaluate the effects of a hot‐water extract of defatted Camellia oleifera seeds (CSE) in carbon tetrachloride (CCl4)‐induced liver damage in rats. Wistar rats were separated into four groups including the normal (N) and CCl4 control (C) groups, which are fed a control diet, and the CCL (low‐dose CSE) and CCH (high‐dose CSE) groups, which are fed with a control diet plus different amount of CSE for an 8‐week experimental period. Liver injury in the C, CCL, and CCH groups was induced by injecting CCl4 (i.p.) twice a week from the 5th week to the end of the study. In CCl4‐treated rats, the alanine transaminase (ALT) and aspartate transaminase (AST) activities and malondialdehyde (MDA) concentration significantly increased compared to the normal group. Lower antioxidative enzyme activities and higher proinflammatory cytokines, transforming growth factor‐β (TGF‐β) and hydroxyproline concentrations in the liver were also found in the CCl4‐treated group compared to the normal group. In contrast, the administration of CSE alleviated the biochemical and histopathological changes including inflammation, liver cell damage, and fibrosis caused by CCl4 in rats. Our results indicated that CSE exhibited hepatoprotective effects in CCl4‐induced liver hepatotoxicity through alleviating hepatic inflammation and fibrosis in rats. Practical Application Camellia oleifera are widely used for edible oil production while the defatted seeds pomace is often discarded. We found that extract of C. oleifera pomace containing phenolic compounds, saponins, and polysaccharides showed protective effects chemical‐driven liver damage and, therefore, may be used in further studies and developing functional foods.

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Comparison of Two Protocols of Carbon Tetrachloride-Induced Cirrhosis in Rats – Improving Yield and Reproducibility

Cited by 55 publications

References 19 publications

Dandelion prevents liver fibrosis, inflammatory response, and oxidative stress in rats

Dandelion prevents liver fibrosis, inflammatory response, and oxidative stress in rats

Hepatoprotective effects and mechanisms of Ixeris denticulate water extract on liver cirrhosis in experimental rat

Camellia Oleifera Seed Extract Mildly Ameliorates Carbon Tetrachloride‐Induced Hepatotoxicity in Rats by Suppressing Inflammation

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