Comparison of two methods for glucocorticoid evaluation in maned wolves

Vasconcellos, Angélica da Silva; Chelini, Marie-Odile Monier; Palme, Rupert; Guimarães, Marco Aurélio; Oliveira, Cláudio Alvarenga de; Ades, César

doi:10.1590/s0100-736x2011001300013

Cited by 15 publications

(11 citation statements)

References 26 publications

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“…This criterion is referred to as the test for parallelism (Beehner and McCann, 2008; Crino et al, 2010; Harper and Austad, 2000; Heilmann et al, 2011; Mateo and Cavigelli, 2005; Sheriff et al, 2011; Vasconcellos et al, 2011). Using serial dilutions of a pooled fecal sample, we checked that assay values varied linearly with hormone concentration within the standard curve boundaries (Buchanan and Goldsmith, 2004; Heilmann et al, 2011) and identified appropriate sample dilutions.…”

Section: Methodsmentioning

confidence: 99%

Effects of population density on corticosterone levels of prairie voles in the field

Blondel

Wallace

Calderone

et al. 2016

General and Comparative Endocrinology

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High population density is often associated with increased levels of stress-related hormones, such as corticosterone (CORT). Prairie voles (Microtus ochrogaster) are a socially monogamous species known for their large population density fluctuations in the wild. Although CORT influences the social behavior of prairie voles in the lab, the effect of population density on CORT has not previously been quantified in this species in the field. We validated a non-invasive hormone assay for measuring CORT metabolites in prairie vole feces. We then used semi-natural enclosures to experimentally manipulate population density, and measured density effects on male space use and fecal CORT levels. Our enclosures generated patterns of space use and social interaction that were consistent with previous prairie vole field studies. Contrary to the positive relationship between CORT and density typical of other taxa, we found that lower population densities (80 animals/ha) produced higher fecal CORT than high densities (240/ha). Combined with prior work in the lab and field, the data suggest that high prairie vole population densities indicate favorable environments, perhaps through reduced predation risk. Lastly, we found that field animals had lower fecal CORT levels than laboratory-living animals. The data emphasize the usefulness of prairie voles as models for integrating ecological, evolutionary and mechanistic questions in social behavior.

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Section: Methodsmentioning

confidence: 99%

Effects of population density on corticosterone levels of prairie voles in the field

Blondel

Wallace

Calderone

et al. 2016

General and Comparative Endocrinology

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“…Fecal glucocorticoid and thyroid hormone metabolites were assayed with commercial [ 125 I] assay kits (MP Biomedicals, Costa Mesa, California). The fecal glucocorticoid metabolite assay was validated by Vasconcellos et al (2011) and the thyroid hormone metabolite assay was validated for recovery by our laboratory (Wasser et al 2010). Fecal progesterone metabolites were assayed with an in-house 3H radioimmunoassay using antibody CL# 425 (Rolland et al 2005), which was previously validated for captive maned wolves (Wasser et al 1995;Velloso et al 1998).…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, adrenocorticotropic hormone (ACTH) challenge studies of fecal glucocorticoid metabolites produced biologically meaningful results in maned wolves (Vasconcellos et al 2011). To understand the influence of landscape use on maned wolf physiology, we correlated hormone levels with environmental and landscape variables including distance to the park or other available natural habitat patches, amount of natural vegetation within 3 km 2 of sample, distance to roads, amount of cropland within 3 km 2 of sample, and crop height at the location of the sample.…”

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confidence: 99%

Physiological implications of landscape use by free-ranging maned wolves (Chrysocyon brachyurus) in Brazil

2014

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“…The maned wolf (Chrysocyon brachyurus) is the largest wild canid of South America and although it is native to the Brazilian cerrado region, it can also be found in Peru, Argentina and Paraguay (Dietz 1985, Estrada et al 2009, Vasconcellos et al 2011). Due to difϐiculties in examining wild animals, there is little information on cardiovascular features in maned wolves.…”

Section: Introductionmentioning

confidence: 99%

Strain and strain rate by two-dimensional speckle tracking echocardiography in a maned wolf

et al. 2012

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Pesq. Vet. Bras. 32(12):1336-1340, dezembro 2012 1336 RESUMO.-[Strain e strain rate por meio de ecocardiograϐia speckle traking bidimensional em um lobo-guará.] A obtenção de parâmetros cardiovasculares em animais selvagens são importantes de serem avaliados, assim como em animais de companhia, para a obtenção da função miocárdica e determinação precoce de alterações cardíacas que poderiam evoluir para insuϐiciência cardíaca . A ecocardiograϐia speckle tracking (2D STE) é uma ferramenta nova que tem sido utilizada em medicina veterinária, a qual tem demonstrado várias vantagens quanto ao seu uso, como a The measurement of cardiovascular features of wild animals is important, as is the measurement in pets, for the assessment of myocardial function and the early detection of cardiac abnormalities, which could progress to heart failure. Speckle tracking echocardiography (2D STE) is a new tool that has been used in veterinary medicine, which demonstrates several advantages, such as angle independence and the possibility to provide the early diagnosis of myocardial alterations. The aim of this study was to evaluate the left myocardial function in a maned wolf by 2D STE. Thus, the longitudinal, circumferential and radial strain and strain rate were obtained, as well as, the radial and longitudinal velocity and displacement values, from the right parasternal long axis four-chamber view, the left parasternal apical four chamber view and the parasternal short axis at the level of the papillary muscles. The results of the longitudinal variables were -13.52±7.88, -1.60±1.05, 4.34±2.52 and 3.86±3.04 for strain (%), strain rate (1/s), displacement (mm) and velocity (cm/s), respectively. In addition, the radial and circumferential Strain and Strain rate were 24.39±14.23, 1.86±0.95 and -13.69±6.53, -1.01±0.48, respectively. Thus, the present study provides the ϐirst data regarding the use of this tool in maned wolves, allowing a more complete quantification of myocardial function in this species.INDEX TERMS: Cardiac function, Chrysocyon brachyurus, maned wolf, systolic function, wild canid. independência do ângulo de insonação e a possibilidade de se obter o diagnóstico precoce de alterações miocárdi-cas. Objetivou-se avaliar a função miocárdica esquerda de um lobo guará por meio do uso da 2D STE. Desta forma, foram obtidas as variáveis Strain e Strain Rate longitudinais, circunferenciais e radiais, assim como a velocidade e o deslocamento longitudinais e radiais, utilizando os cortes eixo longo paraesternal direito quatro câmaras, paraesternal esquerdo apical quatro câmaras e eixo curto paraesternal direito no plano dos músculos papilares. Os resultados das variáveis longitudinais fora -13,52±7,88, -1,60±1,05, 4,34±2,52 e 3,86±3,04 para Strain (%), Strain rate (1/s), deslocamento (mm) e velocidade (cm/s), respectivamente. Quanto ao Strain e Strain rate radiais e circunferenciais foram obtidos 24,39±14,23, 1,86±0,95 e -13,69±6,53, -1,01±0,48. Portanto, o presente estudo fornece os primeiPesq. Vet. Bras. 32(12):1336-1340, de...

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Comparison of two methods for glucocorticoid evaluation in maned wolves

Cited by 15 publications

References 26 publications

Effects of population density on corticosterone levels of prairie voles in the field

Effects of population density on corticosterone levels of prairie voles in the field

Physiological implications of landscape use by free-ranging maned wolves (Chrysocyon brachyurus) in Brazil

Strain and strain rate by two-dimensional speckle tracking echocardiography in a maned wolf

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