Comparison of Two Methods for the Detection of Urinary Iodine Used in Epidemiological Studies

Wuethrich, Christian; Groisman, Sônia; Gerber, H

doi:10.1515/cclm.2000.152

Cited by 16 publications

(8 citation statements)

References 18 publications

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“…UIC was determined by the Sandell±Kolthoff method adapted to modern laboratory equipment (21) by a well trained technical staff that had also performed the analyses of previous Swiss studies (1, 2, 13±16, 21,22). The coefficient of variation of the iodine assays was 7.5% (within-run precision).…”

Section: Laboratory Analysesmentioning

confidence: 99%

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Age- and gender-dependent urinary iodine concentrations in an area-covering population sample from the Bernese region in Switzerland

Als

Keller

Minder

et al. 2000

European Journal of Endocrinology

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Objective: Before a scheduled increase of salt iodide content up to 20 p.p.m. in Switzerland, urinary iodine concentration (UIC, mg/l) of a randomly selected, area-covering, age-stratified population subgroup was sampled in spring 1997. In contrast to former published studies on UIC, we minimized biases by age, gender, rural/urban, socio-economic and cultural influences. Methods: We contacted 750 households in the Bernese region using random telephone numbers. Per household, one proband selected by play dice collected a spot urine sample. We defined seven age categories: infants, children, adolescents, adults I±III and seniors, aged 0±5, 6±12, 13±20, 21±35, 36±50, 51±65 and .65 years respectively. Randomized UIC data were compared with WHO criteria of iodine deficiency (ID) and previous non-randomized, age-dependent Swiss UIC data. To recruit a perfect random group of volunteers proved difficult, as study participation of adolescent and male populations was somewhat lower than that of elder and female P , 0X005 populations respectively. Study participation of inhabitants of rural and suburban regions was comparable P 0X139X Out of 413 obtained urine spots, 412 (55%) were analyzed statistically (58% women, 42% men, P , 0X005). Results: Ninety per cent of all UIC values ranged between 25 and 200 mg/l. Median UIC showed mild ID (94 mg/l). UIC varied significantly between age categories P 0X0006X Women had lower UIC than men P 0X014X Infants had no ID. Women in child-bearing age (13±35 years), adults II, adults III and seniors had mild ID. Compared with recommended supplies of 150 and 200 mg I/day in children and male adolescents respectively, we consider their UIC (110 and 144 mg/l) as at least borderline deficient. Absolute UIC was lowest in seniors. Probands from rural/suburban households had similar UICs. Our UIC data were comparable to published ones. Conclusions: Despite long-term national efforts with iodized salt (15 p.p.m. in 1997), mild ID still prevailed in Bern in spring 1997, with rare extreme UIC values. Mild ID with women in child-bearing age (and probably also with children and male adolescents) was a serious concern, as goiter growth with puberty or pregnancy might be the consequence. Mild ID in adults and seniors might be due to restricted NaCl intake. Results of our randomly selected subgroup from a 650 000 population show that age-and gender-dependency are important when comparing our collected data with published UIC data. This fact must be considered with non-randomly selected population subgroups.

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Section: Laboratory Analysesmentioning

confidence: 99%

“…Absorption was measured after a reaction time of 30 min by an automatic microwell-plate reader (SLT-Labinstruments spectrophotometer) at 405 nm. Three replicates of each sample were analyzed consecutively (21,22).…”

Section: Laboratory Analysesmentioning

confidence: 99%

Age- and gender-dependent urinary iodine concentrations in an area-covering population sample from the Bernese region in Switzerland

Als

Keller

Minder

et al. 2000

European Journal of Endocrinology

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show abstract

“…Automated modes of the Sandell-Kolthoff reaction for measurement of urinary iodine using Technicon AutoAnalyzer II (AAII) systems, with either dialysis or acid digestion, were compared with a method employing manual alkaline ashing. Another comparison of an autoanalyzer method (AAII), where the mineralization takes place in a continuous flow manner with a manual mineralization and discoloration in a 96-well microtiter plate read by a PC-controlled photometer, was performed by Wuethrich et al (2000). However, a comparison of five methods, with both manual and improved automatic digestion, as well as with both manual and automated reading of the Sandell-Kolthoff reaction, yielded agreement among the methods tested, and also with an inductively coupled plasma mass spectrometry method (May et al, 1997).…”

Section: Catalytic Spectrophotometric Methodsmentioning

confidence: 99%

Assay of Iodine in Foodstuffs

Kučera¹

2009

Comprehensive Handbook of Iodine

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“…These can provide high sample throughput and reduce the amount of waste per sample [9][10][11], although the Technicon Autoanalyzer and equivalent segmented flow analyzers are still in use [7].…”

Section: The Sandell-kolthoff Reactionmentioning

confidence: 99%