Comparison of Two Different Nutritive Conditions against the Changes in Peripheral Blood Mononuclear Cells of Periparturient Dairy Cows

Abstract

ABSTRACT. To clarify the relationship between cellular immune status and nutritive condition in periparturient dairy cows, feeding content, blood profiles, and immune condition were observed in cows from two dairy herds with different types of feed content. Immunological analyses such as leukocyte population and peripheral blood mononuclear cell (PBMC) mRNA of IFN-γ, TNF-α, IL-4, and IL-10, quantified by real-time RT-PCR were performed. With regard to feed content during dry periods, there were six cows in the herd with insufficient non-structural carbohydrate (NFC) intake (group I) and six cows in the herd with sufficient NFC intake (group II). Significantly lower levels of blood glucose were observed in group I between weeks -12 and 16 compared with group II. Serum cholesterol level was significantly lower in group I between weeks 2 and 10 than in group II. The numbers of CD3 + and CD4 + T cells in group I were significantly lower than those in group II in weeks 6 and 14. The numbers of CD21 + B cells were significantly lower in group I than in group II in weeks -16, -12, 2, and 10. On the other hand, the CD4 + /CD8 + ratio in group II was significantly higher than group I between weeks 2 and 14. The IFNγ/IL-4 mRNA rate in group I was significantly lower than group II in week 6. We concluded that cellular immune depression occurrs after calving in dairy cows with low nutritional status in the periparturient period. KEY WORDS: dairy cow, immune function, nutritive conditions, periparturient.

Effect of Lidocaine Hydrochloride on the Function of Bovine Peripheral Leukocytes

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Changes in Peripheral Blood Mononuclear Cells after Calving in Lactating Cows with Serious Decrease of Body Weight before and after Calving

“…The real-time PCR method has been previously described in detail [16,21]. We placed 10 6 PBMC in 1 ml of 10%FCS-RPMI medium into a 24-well plate, and stimulated the cells with phytohemagglutinin (PHA, Sigma, 5 µg/ml) for 12 hr at 37°C.…”

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Relationship between Immune Function and Serum Vitamin A in Japanese Black Beef Cattle

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“…Real-time PCR was used to measure the mRNA levels of cytokines as previously described [17,19]. Two micrograms of total RNA from each sample were used for synthesis of first-strand cDNA with oligo-dT primers (Invitrogen, Carlsbad, CA, U.S.A.) and Superscript II Reverse Transcripts (Invitrogen, Carlsbad, CA, U.S.A.) according to the manufacturer's protocols.…”