Effect of Lidocaine Hydrochloride on the Function of Bovine Peripheral Leukocytes

Abstract

ABSTRACT. To clarify the effect of lidocaine hydrochloride (Lid) on bovine peripheral granulocyte phagocytosis, adhesion molecule expression of leukocytes and peripheral blood mononuclear cell mRNA expression of cytokines were investigated. Lid was added to blood samples at a final concentration of 0 (only PBS; Cont), 0.2 mg/ml or 2.0 mg/ml. Phagocytosis of granulocytes was significantly decreased by addition of 2.0 mg/ml of Lid. CD18 expression of granulocytes and mononuclear cells were significantly reduced by addition of 2.0 mg/ml of Lid. IL-1 and IL-8 mRNA expressions of mononuclear cells were also significantly reduced by addition of 2.0 mg/ml of Lid other hand. These results suggest that Lid might reduce the protective immunity of cows. On the other hand, reduction of CD18, IL-1 and IL-8 mRNA expression also indicates that Lid has an anti-inflammatory effect in cows. KEY WORDS: adhesion molecules, anti-inflammatory effect, cattle, lidocaine hydrochloride, phagocytosis.

The Effect of Intravenous Lidocaine Infusion on Bronchoalveolar Lavage Cytology in Equine Recurrent Airway Obstruction

“…However, at the latter dosage, lidocaine does not exert its beneficial effect as a consequence of impaired neutrophil influx into intestinal mucosa, although other anti‐inflammatory mechanisms may be operative . Because in vitro studies of bovine and canine peripheral blood demonstrate decreased adhesion molecule expression in a dose‐dependent manner and as the threshold for serum lidocaine toxicity is double that of 980 ng/mL, we elected to deliver a higher dosage to potentially maximize our ability to detect a significant lidocaine effect on neutrophilic inflammation. However, in rabbit studies of endotoxin‐induced acute lung injury, delivery of IV lidocaine at 0.03 mg/kg/min (plasma lidocaine concentrations not provided) significantly decreases neutrophilic infiltration into pulmonary parenchyma.…”

“…Lidocaine also decreases expression of adhesion molecules, which impairs neutrophil extravasation. [1][2][3] Prophylactic lidocaine therapy has shown promise in animal models of acute lung injury where it decreases pulmonary neutrophilic inflammation. 4 Asthmatic patients treated with nebulized lidocaine show improved clinical signs and lung function and decreased reliance on bronchodilator therapy.…”

“…In particular, lidocaine impairs neutrophil function by decreasing priming, respiratory burst, superoxide production, and production of cytokines. Lidocaine also decreases expression of adhesion molecules, which impairs neutrophil extravasation . Prophylactic lidocaine therapy has shown promise in animal models of acute lung injury where it decreases pulmonary neutrophilic inflammation .…”

A Review of the Effects of Pain and Analgesia on Immune System Function and Inflammation: Relevance for Preclinical Studies

“…82 PMN and macrophage functions (including chemotaxis, adherence, production of toxic oxygen species, phagocytosis, and cytokine release) are inhibited by LAs. 10,12,13,70,84,191 Lidocaine has been shown to inhibit cell proliferation, cytokine production, and mitogen-activated protein kinase activation in T cells and upregulate regulatory T-cells that promote an antiinflammatory t-cell phenotype. 84,94,101,118 One study in mouse showed that release of antiinflammatory cytokine IL10 may be enhanced by lidocaine.…”