Comparison of two different deposition methods of 3-aminopropyltriethoxysilane on glass slides and their application in the ThinPrep cytologic test

Abstract

In this work, two different deposition methods of 3-aminopropyltriethoxysilane (APTES) on glass slides were compared in order to study the adhesion effect of cervical exfoliated cells on smear slides. Glass slides were modified by vapor-phase deposition (V-D) and liquid-phase deposition (L-D), respectively. The topographic images and amine density of the modified slides were investigated by using atomic force microscopy, UV-vis spectroscopy and X-ray photoelectron spectroscopy. The numbers of cells adhered on the slides functionalized by V-D and L-D were counted and compared under the microscope. The data showed significant differences between the two methods (t-test: P < 0.05). The results presented here have made it theoretically possible to produce amine slides by V-D method for the ThinPrep cytologic test.

Fluorinated Boron‐Dipyrromethene (BODIPY) Dyes: Bright and Versatile Probes for Surface Analysis

“…Considering that based on the van der Waals radii of common organic dye molecules such as rhodamines or BODI-PYs the theoretically achievable surface coverage amounts to 2.5 10 13 per cm 2 , [25] the present labelling ratio of only approximately 3 % suggests that the high-loading deposition procedure adapted from Ref. [76] indeed produces APTES films, which are thicker than a monolayer. Such a behaviour has been reported before for APTES and other deposition techniques.…”

“…For this purpose, amino slides were prepared from cleaned and activated commercial glass slides using a vapour deposition procedure adapted from Ref. [76]. The amount of 3-aminopropyltriethoxysilane (APTES) used in the functionalization procedures was chosen in such a way that a quantitative surface functionalization is reached.…”

“…If we assume that the amino group density achieved under the high-loading deposition conditions as employed here amounts to ! 3 10 14 molecules per cm 2 , [76,83] the concentration of labelled amino groups derived from slide D 60 data of [NH 2 ] C~3 % according to Equations (1) and (2) indicates that about 9 10 12 NH 2 groups per cm 2 have been successfully labelled with 5. Considering that based on the van der Waals radii of common organic dye molecules such as rhodamines or BODI-PYs the theoretically achievable surface coverage amounts to 2.5 10 13 per cm 2 , [25] the present labelling ratio of only approximately 3 % suggests that the high-loading deposition procedure adapted from Ref.…”

Conducting polymer-based multilayer films for instructive biomaterial coatings

“…Aminopropyltrimethoxysilane-functionalized glass microscope slides were used as positively charged substrates for the deposition of conducting polymer-based films [21]. Negatively charged polymers were either poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) 1.1% in H 2 O, neutral pH, high-conductivity grade (PEDOT-PSS), or PEDOT-S; whereas the positively charged polymer was chitosan (70 kDa) that was dissolved in aqueous acetic acid (1% v/v).…”

ZnO Nanowire Arrays Exhibit Cytotoxic Distinction to Cancer Cells with Different Surface Charge Density: Cytotoxicity is Charge‐Dependent

“…Cellular toxicity of 1‐D nanostructures were mainly focused on dose addition, aspect ratio, and modulus of the nanomaterials . Charge property of nanomaterials should also present significant impact on cells, because cells are always negatively charged on surface . Therefore, electrical interaction between nanomaterials and cells should be reasonably important to cytotoxicity.…”