Conducting Polymer-Based Multilayer Films for Instructive Biomaterial Coatings

Hardy, John G.; Li, Hetian; Chow, Jacqueline K.; Geissler, Sydney A.; McElroy, Austin; Nguy, Lindsey; Hernandez, Derek S.; Schmidt, Christine E.

doi:10.4155/fso.15.79

Cited by 15 publications

(5 citation statements)

References 39 publications

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“…Schmidt and co-workers, showed the alignment of human dermal fibroblast is enhanced by a DC current (Hardy et al, 2015). We also observed cell alignment results are quite similar to the distribution of actin filament orientation, in the same condition.…”

Section: Resultssupporting

confidence: 72%

Electrical Stimulation Changes Human Mesenchymal Stem Cells Orientation and Cytoskeleton Organization

Mobini

Talts

Xue³

et al. 2017

j biomater tissue eng

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Electricity, as a physical stimulus, is recently becoming an attractive tool for tissue engineering. In this study we simulated the electrical field delivered by a simplified electro-bioreactor, using finite element analysis. In addition, human mesenchymal stem cells (hMSCs) were cultured in an electro-bioreactor and an electrical field of 100 mV/mm for 1 hour per day was applied. The cell profile, orientation and cytoskeleton changes by CellProfiler was analysed at 1, 2, 3 and 7 days. The cytoskeleton texture of cells exposed to electrical stimulation was also compared with cells exposed to chemical stimulation during an early phase of osteogenic differentiation. Results showed that hMSCs orientation and cytoskeleton actin filaments reorganize perpendicular to the electrical field in the vicinity of the cathode area at day 7. This finding and analysis method has the potential to provide a framework for future studies of mechanism underlying the changes in cell profile in electrical fields.

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Section: Resultssupporting

confidence: 72%

Electrical Stimulation Changes Human Mesenchymal Stem Cells Orientation and Cytoskeleton Organization

Mobini

Talts

Xue³

et al. 2017

j biomater tissue eng

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“…It is known that the charge density on the polymer and the ionic strength in aqueous media influence the hydrogel properties such as the swelling ratio and the elastic modulus [ 10 , 11 , 12 ]. For hydrogels and nanoparticles, the negative charge of polyanions such as hyaluronan can be utilized by (a) ionic cross-linking with polycations such as polyallylamine hydrochloride or modified chitosan [ 13 , 14 , 15 ]; (b) ionic cross-linking with a low molecular weight cation [ 16 ]; (c) covalent cross-linking with a polycation or a cationic dendrimer [ 17 ]; or (d) photochemical cross-linking of methacrylate-functionalized HA with an unsaturated polycation [ 18 ]. These materials provide various applications such as gene transfection [ 17 ], biosensors for enzymatic reactions [ 16 ], and films for uni-directional drug delivery and controlled release [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Charged Triazole Cross-Linkers for Hyaluronan-Based Hybrid Hydrogels

et al. 2016

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Polyelectrolyte hydrogels play an important role in tissue engineering and can be produced from natural polymers, such as the glycosaminoglycan hyaluronan. In order to control charge density and mechanical properties of hyaluronan-based hydrogels, we developed cross-linkers with a neutral or positively charged triazole core with different lengths of spacer arms and two terminal maleimide groups. These cross-linkers react with thiolated hyaluronan in a fast, stoichiometric thio-Michael addition. Introducing a positive charge on the core of the cross-linker enabled us to compare hydrogels with the same interconnectivity, but a different charge density. Positively charged cross-linkers form stiffer hydrogels relatively independent of the size of the cross-linker, whereas neutral cross-linkers only form stable hydrogels at small spacer lengths. These novel cross-linkers provide a platform to tune the hydrogel network charge and thus the mechanical properties of the network. In addition, they might offer a wide range of applications especially in bioprinting for precise design of hydrogels.

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“…The material is also well characterised with biocompatible properties in vitro/vivo, which opens up a range of opportunities for soft tissue augmentation and regeneration [ 20 , 21 , 22 ]. Hence, there is increased interest in several research programmes mainly in regulating cell behaviour through electrical stimulation and in helping to trigger cell desorption [ 44 , 49 ].…”

Section: Introductionmentioning

confidence: 99%

Conductive Bioimprint Using Soft Lithography Technique Based on PEDOT:PSS for Biosensing

et al. 2021

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Culture platform surface topography plays an important role in the regulation of biological cell behaviour. Understanding the mechanisms behind the roles of surface topography in cell response are central to many developments in a Lab on a Chip, medical implants and biosensors. In this work, we report on a novel development of a biocompatible conductive hydrogel (CH) made of poly (3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) and gelatin with bioimprinted surface features. The bioimprinted CH offers high conductivity, biocompatibility and high replication fidelity suitable for cell culture applications. The bioimprinted conductive hydrogel is developed to investigate biological cells’ response to their morphological footprint and study their growth, adhesion, cell–cell interactions and proliferation as a function of conductivity. Moreover, optimization of the conductive hydrogel mixture plays an important role in achieving high imprinting resolution and conductivity. The reason behind choosing a conducive hydrogel with high resolution surface bioimprints is to improve cell monitoring while mimicking cells’ natural physical environment. Bioimprints which are a 3D replication of cellular morphology have previously been shown to promote cell attachment, proliferation, differentiation and even cell response to drugs. The conductive substrate, on the other hand, enables cell impedance to be measured and monitored, which is indicative of cell viability and spread. Two dimensional profiles of the cross section of a single cell taken via Atomic Force Microscopy (AFM) from the fixed cell on glass, and its replicas on polydimethylsiloxane (PDMS) and conductive hydrogel (CH) show unprecedented replication of cellular features with an average replication fidelity of more than 90%. Furthermore, crosslinking CH films demonstrated a significant increase in electrical conductivity from 10−6 S/cm to 1 S/cm. Conductive bioimprints can provide a suitable platform for biosensing applications and potentially for monitoring implant-tissue reactions in medical devices.

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Conducting Polymer-Based Multilayer Films for Instructive Biomaterial Coatings

Cited by 15 publications

References 39 publications

Electrical Stimulation Changes Human Mesenchymal Stem Cells Orientation and Cytoskeleton Organization

Electrical Stimulation Changes Human Mesenchymal Stem Cells Orientation and Cytoskeleton Organization

Charged Triazole Cross-Linkers for Hyaluronan-Based Hybrid Hydrogels

Conductive Bioimprint Using Soft Lithography Technique Based on PEDOT:PSS for Biosensing

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