Comparison of turbidimetric and light-scattering measurements of immunoglobulins by use of a centrifugal analyzer with absorbance and fluorescence/light-scattering optics.

Hills, Lorin P.; Tiffany, T. O.

doi:10.1093/clinchem/26.10.1459

Cited by 37 publications

(10 citation statements)

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“…Growth hormone, insulin, prolactin and progesterone concentrations of plasma were determined as described by Vernon, Clegg & Flint (1981), plasma cortisol according to Flint, Clegg & Knight (1984) and plasma oestradiol-17/7 using a kit supplied by CIS (U.K.) Ltd, London. The concentrations of plasma glucose and of colostral lactose and lipid were determined as described by Mellor & Murray (1985b) and colostral immunoglobin G according to Hills & Tiffany (1980). Placental lactogen was measured by a double-antibody radioimmunoassay (Thordarson, 1984).…”

Section: Hormone and Metabolite Analysesmentioning

confidence: 99%

Relationships Between Plasma Hormone Concentrations, Udder Development and the Production of Early Mammary Secretions in Twin‐bearing Ewes on Different Planes of Nutrition

et al. 1987

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SUMMARYEwes carrying twin fetuses were maintained during late pregnancy on a uniformly high plane (well fed), a uniformly low plane (underfed) or a low plane rising to a high plane (refed) of nutrition. The maternal plasma concentrations of hormones concerned with udder development, colostrum production and milk secretion were measured in samples obtained during the last 32 d before and the first 18 h after birth. Udder weights were derived from their linear dimensions, the yields of mammary secretions by hand milking during the first 18 h after birth and the transition from colostrum to milk was indicated by changes in the concentrations of lactose, lipid and immunoglobulin G. Underfeeding reduced prenatal udder development and colostrum accumulation and delayed the post-natal transition to milk secretion. Refeeding previously underfed ewes to a high plane during the last 5 d before birth had no effect on udder growth, but it did return the secretory functions of udder tissue to the levels observed in well-fed ewes. Nutritional effects on the plasma concentrations of insulin, placental lactogen, prolactin, cortisol and oestradiol-17/7 were small or absent. The relative changes in the plasma concentrations of growth hormone and insulin were such that the growth hormone: insulin ratio increased markedly during the last 11 d before birth in the underfed and refed ewes but not in the well-fed ewes. These differences in the growth hormone: insulin ratio were associated with greater increases in udder growth rate in the underfed and refed ewes than in the well-fed ewes. The plasma concentrations of progesterone decreased before birth in all ewes, but the decrease was delayed by underfeeding and refeeding rapidly overcame this effect. The slower progesterone withdrawal in the underfed ewes was associated with a delay in lactogenesis. It is concluded that the nutritionally induced changes in the growth hormone: insulin ratio and in the progesterone concentrations were the most likely endocrine determinants of the observed differences in the rates of mammogenesis and lactogenesis, respectively, but possible independent effects of substrate supply to the udder remain to be'clarified.

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Section: Hormone and Metabolite Analysesmentioning

confidence: 99%

Relationships Between Plasma Hormone Concentrations, Udder Development and the Production of Early Mammary Secretions in Twin‐bearing Ewes on Different Planes of Nutrition

et al. 1987

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“…The reagents and conditions for the turbidimetric measurement of equine IgG were adapted from Finlay, Williams and Byers (1976), Devenll (1980) and Hills and Tiffany (1980) using antiserum produced as above. Six microlitres of dilute serum was added to 125 pl of diluted sheep anti-IgG serum at 37°C and the change in optical density measured, at 60 sec intervals for 240 secs at 340 nm, on a centrifugal analyser (Multistat 111 ; Instrumentation Laboratories, Warrington, Cheshire).…”

Section: Turbidimetric Analysismentioning

confidence: 99%

Measurement of IgG in equine blood by immunoturbidimetry and latex agglutination

Kent

Blackmore

1985

Equine Veterinary Journal

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Summary This paper describes the quantitative measurement of IgG concentrations in equine blood/serum by turbidimetric analysis and the qualitative assessment using latex agglutination. The concentration of IgG in foal serum measured by turbidimetry correlated well with the results obtained by radial immunodiffusion (r = 0.91) and the γ‐globulins estimated from the electrophoresis of serum on cellulose acetate (r = 0.92). The method, using antibody‐coated latex beads, to detect foals with serum IgG concentrations of less than 4 g/litre (whole blood less than 2 g/litre) proved to be accurate in 96 per cent of cases and provided a quick and simple qualitative test, suitable for use outside the laboratory.

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“…The use of the Multistat system for measuring proteolytic enzymes with fluorogenic peptide sub strates is reported by Pearson et al 102 in which they describe the accuracy, reproducibility, and speed of this system. Another report by Hills and co workers 62 compares the measurement of immuno globulins through light scatter techniques on centri fugal analyzers. They report that both nephelometry and turbidimetry can be satisfactorily adapted to centrifugal analyzers.…”

Section: Lal Proenzyme Enzymementioning

confidence: 99%

Automated Instrumentation and the Laboratory Diagnosis of Bleeding and Thrombotic Disorders

Walenga¹,

Fareed²,

Bermes³

1983

Semin Thromb Hemost

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Comparison of turbidimetric and light-scattering measurements of immunoglobulins by use of a centrifugal analyzer with absorbance and fluorescence/light-scattering optics.

Cited by 37 publications

References 0 publications

Relationships Between Plasma Hormone Concentrations, Udder Development and the Production of Early Mammary Secretions in Twin‐bearing Ewes on Different Planes of Nutrition

Relationships Between Plasma Hormone Concentrations, Udder Development and the Production of Early Mammary Secretions in Twin‐bearing Ewes on Different Planes of Nutrition

Measurement of IgG in equine blood by immunoturbidimetry and latex agglutination

Automated Instrumentation and the Laboratory Diagnosis of Bleeding and Thrombotic Disorders

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